国际肿瘤学杂志

国际肿瘤学杂志 ›› 2017, Vol. 44 ›› Issue (1): 6-10.doi: 10.3760/cma.j.issn.1673-422X.2017.01.002

• 论著 • 上一篇    下一篇

血清DKK1和EB病毒VCAIgA联合检测对鼻咽癌的诊断价值

许镒洧, 黄利生, 郭海鹏, 彭裕辉   

  1. 515041汕头大学医学院附属肿瘤医院检验科（许镒洧、彭裕辉），放疗科（黄利生），头颈外科（郭海鹏）
  • 出版日期:2017-01-08 发布日期:2016-12-07
  • 通讯作者: 彭裕辉，Email: pengyuhui666@163.com E-mail:pengyuhui666@163.com
  • 基金资助:

    广东省科技计划（2013B021800250）； 汕头大学医学院临床研究提升计划（201428）

Diagnostic value of combined detection of serum DKK1 and EB virus VCA-IgA for nasopharyngeal carcinoma

Xu Yiwei, Huang Lisheng, Guo Haipeng, Peng Yuhui   

  1. Department of Clinical Laboratory, Cancer Hospital of Shantou University Medical College, Shantou 515041, China
  • Online:2017-01-08 Published:2016-12-07
  • Contact: Peng Yuhui, Email: pengyuhui666@163.com E-mail:pengyuhui666@163.com
  • Supported by:

    Science and Technology Program of Guangdong Province (2013B021800250); Clinical Promotion Plan of Shantou University Medical College (201428)

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摘要： 目的 探讨血清Dickkopf-1（DKK1）和EB病毒壳抗体（VCA-IgA）联合检测在鼻咽癌中的诊断价值。方法 应用酶联免疫吸附试验检测80例鼻咽癌患者和65例正常对照者血清DKK1和VCA-IgA的水平，采用受试者工作特征（ROC）曲线评价诊断效能。结果 鼻咽癌患者血清DKK1的表达水平明显高于正常对照组［580.773（429.146）pg/ml∶316.174（252.965）pg/ml］，差异有统计学意义（Z=4.846，P＜0.000 1）。ROC曲线显示血清DKK1对鼻咽癌的最佳诊断临界值为611.981 pg/ml，其诊断曲线下面积（AUC）为0.734（95%CI为0.654～0.815），敏感性为50.0%，特异性为96.9%。VCA-IgA对鼻咽癌的诊断AUC为0.714（95%CI为0.631～0.798），敏感性为47.5%，特异性为95.4%。DKK1和VCA-IgA联合检测的诊断AUC为0.849（95%CI为0.783～0.914），敏感性为76.3%，特异性为95.4%。早期鼻咽癌患者DKK1和VCA-IgA联合检测效果优于正常对照组，差异有统计学意义（χ2=23.784，P＜0.001）。结论 血清DKK1对鼻咽癌具有潜在的诊断价值，联合检测VCA-IgA有助于鼻咽癌的早期诊断。

关键词: 鼻咽肿瘤, 诊断, Dickkopf-1, EB病毒壳抗体

Abstract: Objective  To explore the diagnostic value of the combined detection of serum Dickkopf-1（DKK1） and EB viral capsid antigen immunoglobulin A (VCA-IgA) in patients with nasopharyngeal carcinoma (NPC). Methods Serum levels of DKK1 and VCA-IgA were measured by enzymelinked immunosorbent assay (ELISA) for the 80 patients with NPC and 65 normal controls. Receiver operating characteristic (ROC) curve was used to calculate the diagnostic value. Results The serum levels ［M(QR)］ of DKK1 in patients with NPC were significantly higher than those in normal controls ［580.773(429.146)pg/ml vs. 316.174(252.965)pg/ml］, with a significant difference (Z=4.846, P＜0.000 1). ROC curves showed that the optimum diagnostic cutoff for serum DKK1 was 611.981 pg/ml, with an area under curve (AUC) of 0.734 (95%CI: 0.654-0.815, 50.0% sensitivity, 96.9% specificity). Measurement of VCA-IgA demonstrated an AUC of 0.714 (95%CI: 0.631-0.798, 47.5% sensitivity, 95.4% specificity). The combined detection of DKK1 and VCA-IgA demonstrated an AUC of 0.849 (95%CI: 0.783-0.914, 76.3% sensitivity, 95.4% specificity). For patients with early-stage NPC, the detection effect of combined detection of DKK1 and VCA-IgA was much better than that in normal controls, with a significant difference (χ2=23.784, P＜0.001). Conclusion Serum DKK1 has potential diagnostic value for NPC. Combined detection of DKK1 and VCA-IgA may aid the early diagnosis of NPC.

Key words: Nasopharyngeal neoplasms, Diagnosis, Dickkopf-1, EB viral capsid antigen immunoglo-bulin A