Abstract: ObjectiveTo investigate the effect of knockingdown Yesassociated protein (YAP) on the biological characteristics of SNB19 glioblastoma cell. MethodsThe expression of YAB in SNB19 was knockdown by YAB small interfering RNA (YABsiRNA). The downregulation of YAP expression was identified by Western blot analysis. The proliferative ability of cell was determined by methyl thiazoyl terazolium (MTT). The invasive ability of cell was examined by Transwell assay. Flow cytometry and Annexin V staining were used to detect the cell cycle and apoptosis respectively. The results were analyzed by the statistical software SPSS18.0. ResultsThe expression of YAP in the cells transfected with YAPsiRNA was significantly reduced. The cell proliferation activity of SNB19 cells was inhibited, which decreased from (100.00±0.00)% to (52.32±3.10)% (F=33.00, P<0.01). The cell cycle was arrested in G0G1 phase (F=8.76, P<0.01). The cell invasive ability was attenuated apparently, which decreased from (163.20±10.10) to (37.71±2.52) (F=282.05, P<0.01). The apoptosis ratio of the tumor cell which transfected with YAPsiRNA was increased from (3.56±0.35)% to (18.99±0.66)%, (F=931.99, P<0.01). ConclusionKnockingdown YAP expression in glioma cells could inhibit the proliferative activity and invasive ability of SNB19 cell and could induce cell apoptosis. YAP could be served as a potential target for the gene therapy of glioma.

Key words: Glioma, Cell proliferation, Apoptosis, Yes-associated protein