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25 September 2014, Volume 41 Issue 9 Previous Issue    Next Issue

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Expression and regulatory mechanism of microRNA-133 in tumor Zhang Yue, Zhao Liang 2014, 41 (8):  641-644.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.001 Abstract ( 434 )   PDF (781KB) ( 1448 )   Save As small non-coding RNAs, microRNAs (miRNAs) have been recognized as important regulatory factors in the posttranscription regulation network. Studies have shown that miR-133a and miR-133b, as members of miR-133 family, can regulate the expression of target genes such as epidermal growth factor receptor and oncogene. They regulate the mitogen-activated protein kinases and protein kinase B signaling pathways, which affect tumor cell proliferation, invasion and migration. MiR-133 plays a key role in the process of tumor development and progression, suggesting that it could be served as a new target for cancer treatment. References | Related Articles | Metrics

Functions of platelets in tumor growth and metastasis Zhang Tianjia, Dang Suying 2014, 41 (8):  645-647.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.002 Abstract ( 589 )   PDF (689KB) ( 1821 )   Save Increases of platelet amount and activation are associated with tumor metastasis. Current studies reveal that platelets participate in the process of tumor metastasis via promoting immune escape, adhesion and angiogenesis. The direct interactions between platelets and tumor cells are also founded as an important determinant in metastasis. The functions of platelets in tumor progression and metastasis suggest that platelets could be served as new potential targets for cancer treatment. References | Related Articles | Metrics

Alternative splicing of tumor associated genes messenger RNA and application Zhang Xintong, Yue Wentao 2014, 41 (8):  648-651.  doi: 10.3760/cma.j.issn.1673422X.2014.09.003 Abstract ( 480 )   PDF (698KB) ( 1359 )   Save As a way of gene modification, alternative splicing is an important factor of eukaryotic gene expression and regulation. It makes various transcripts from one proteincoding gene, and greatly extends the genetic information. Alternative splicing of premessenger RNA plays an important role in tumor cells. By alternative splicing, some important genes can generate splicing variants different from those in normal cells. The existence of tumorspecific splicing variants leads to the occurrence and progression of tumor. Therefore, exploration on the alternative splicing of tumorassociated genes may be of great significance in tumor diagnosis and treatment. References | Related Articles | Metrics

Applications and mechanism of some clinical common medications used as anti-cancer therapy sensitizer Li Rutian, Qian Xiaoping, Liu Baorui 2014, 41 (8):  651-655.  doi: 10.3760/cma.j.issn.1673422X.2014.09.004 Abstract ( 437 )   PDF (708KB) ( 1073 )   Save A number of medications have been proved to be able to either improve the antitumor effect of chemotherapeutics and molecular targeted drugs or reverse the resistance of tumors to chemotherapeutics and molecular targeted drugs, which are not traditionally used as anticancer drugs. Especially for late-stage tumors after multiple treatments, these agents are good alternatives when used independently or in combination with chemotherapeutics and molecular targeted drugs. These drugs include proton modulators, hypoglycemic agents and cardiovascular agents, etc. References | Related Articles | Metrics

Successful patterns of maintenance therapy in human common cancer Zhang Baihong, Yue Hongyun 2014, 41 (8):  655-658.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.005 Abstract ( 606 )   PDF (700KB) ( 1977 )   Save Maintenance therapy is an important strategy for cancer treatment,  which mainly include continuation maintenance therapy and switch maintenance therapy, with a classical model of metronomic chemotherapy. Maintenance therapy can be an effective treatment option for patients with nonsmall cell lung cancer, breast cancer, colorectal cancer and ovarian cancer. References | Related Articles | Metrics

Basis and clinical research of 125I seed implantation for curing nasopharyngeal carcinoma Ma Xuan, Li Xiaojiang 2014, 41 (8):  659-661.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.006 Abstract ( 491 )   PDF (693KB) ( 1413 )   Save Radiotherapy is an important means for curing nasopharyngeal carcinoma at present. However, how to handle the focus residual, relapse and metastasis after the radiotherapy of nasopharyngeal carcinoma is still a hot potato. 125I seed implantation is regarded as a member of the comprehensive therapies. Its feasibility for curing such cancer has already been confirmed in the experimental research of the cytology and zoology, and it has been applied in the focus residual, relapse and metastasis after the radiotherapy. Furthermore, its clinical therapy effect has been acknowledged. References | Related Articles | Metrics

Molecular targeted therapy of triple-negative breast cancer Gong Liya, Chen Hongfeng 2014, 41 (8):  662-665.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.007 Abstract ( 559 )   PDF (702KB) ( 1332 )   Save Treatment options are limited for triple negative breast cancer (TNBC) since endocrinotherapy and targeted therapy that aims directly at human epidermal growth factor receptor-2 (HER-2) are ineffective. As such, in addition to surgical treatment, the mainstay of treatment of TNBC is systemic cytotoxic chemotherapy. The targeted therapy of TNBC is becoming a research hotspot because of traditional chemotherapy curative effect is not good enough. A large number of clinical trials have found that patients with TNBC can get benefits from targeted molecular strategies including polyadenosine diphosphate glucose pyrophospheralase-ribose polymerase-1 (PARP-1) inhibitor and epidermal growth factor receptor (EGFR) inhibitor. References | Related Articles | Metrics

ERCC1 gene polymorphism in gastric cancer Tian Yuan, Shan Zezhi, Peng Jiayuan, Wang Yu 2014, 41 (8):  666-668.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.008 Abstract ( 552 )   PDF (692KB) ( 1358 )   Save As a crucial part of the DNA damage repair process, the expression of excision repair crosscomplementing group 1 (ERCC1) is closely related to the genesis and development of gastric cancer. Studies find that ERCC1 gene polymorphism can alter the expression of the gene itself, which affects the sensitivity and efficacy of platinumbased chemotherapy. Therefore, the detection of ERCC1 polymorphism may guide the individualized chemotherapy of the patients with gastric cancer. References | Related Articles | Metrics

Markers of renal cell carcinoma stem cells Huang Liangliang, Wang Delin 2014, 41 (8):  668-671.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.009 Abstract ( 410 )   PDF (700KB) ( 1375 )   Save The renal cell cancer stem cells determine the growth and proliferation of renal cell carcinoma. So far some possible markers have been identified in renal cell carcinoma, including octamer binding factor 4 (Oct4), CD133, CD105, ATPbinding cassette subfamily B member 1 transporter gene (ABCB1), CXC chemokine receptor 4 (CXCR4), but these markers are still controversial. Therefore, for the more effective treatments of metastatic renal cell carcinoma, studying a generally applicable marker for renal cell carcinoma is necessary. References | Related Articles | Metrics

Targeted therapy of metastatic renal cell cancer Wang Hongbiao, Li Xuyuan, Lin Wenzhao 2014, 41 (8):  672-674.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.010 Abstract ( 417 )   PDF (690KB) ( 1187 )   Save Targeted therapy of antiangiogensis strategy is the standard treatment for metastatic renal cell cancer. In recent years, a number of new generation of antiangiogensis agents have been tested in clinical trials, some of which have achieved promising outcomes. Other pathway inhibitors such as inhibitors of mammalian target of rapamycin and fibroblast growth factor receptor pathway have made progresses in some extent. References | Related Articles | Metrics

Characteristics, diagnosis and treatment of interdigitating dendritic cell sarcoma Li Junping, Zhao Rusen, Wang Yongtao, Li Bing, Zhang Guizhi 2014, 41 (8):  675-678.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.011 Abstract ( 1016 )   PDF (698KB) ( 1646 )   Save Interdigitating dendritic cell sarcoma (IDCS) is a rare malignant tumor of the dendritic cell, derived from the hematopoietic tissue. The major clinical manifestation of IDCS is superficial lymphadenopathy, and the enlarged lymph nodes may appear in some atypical ereas, such as the lung, kidney, bladder and the pleura, etc. With the development of the pathological diagnosis and the application of immunohistochemical staining and electron microscopes, the case detection rate is apparently improved. With the high degree of malignant, rapid progress and poor prognosis of the disease, currently, surgical therapy is still the main approach to the treatment of IDCS. References | Related Articles | Metrics

Roles and mechanisms of Fas/FasL in the apoptosis of HL-60 cells induced by euphorbiasteroid Guo Fei, Li Xia, Zhang Chao, Ren Xia, Shi Meiyan, Jiang Guosheng 2014, 41 (8):  679-684.  doi: 10.3760/cma.j.issn.1673422X.2014.09.012 Abstract ( 364 )   PDF (1575KB) ( 1461 )   Save ObjectiveTo investigate the effect of euphorbiasteroid on inducing the apoptosis of HL60 cells and demonstrate whether the Fas/FasL signaling pathway is involved in the induction of apoptosis. MethodsHL60 cells were treated with dose of 2.5, 10, 40 μg/ml of euphorbiasteroid in vitro for 24 h respectively. After that, cell counting Kit8 was used to detect cell proliferation. The morphology of HL60 cells were observed under light and fluorescent microscopy. The early cell apoptosis was detected by using flow cytometry with Annexin ⅤFITC /PI double staining. The expressions of Fas, FasL, caspase8 and caspase3 mRNA were analyzed by the method of RTPCR. The activities of caspase8 and caspase3 were examined by chromatometry. ResultsCompared with 1640 control group, HL60 cell proliferation was inhibited significantly by euphorbiasteroid. The inhibition rates were (34.9±3.7)%, (54.6±5.2)% and (61.3±4.3)% respectively. Moreover, HL60 cells exhibited typical morphological features. Early cell apoptosis rates of HL60 cells were (23.4±3.1)%, (35.7±4.3)% and (53.2±3.9)% respectively. Furthermore, the expressions of Fas, FasL, caspase3 and caspase8 mRNA were upregulated significantly after euphorbiasteroid administration in a dosedependent manner (P＜0.01). After treated with euphorbiasteroid, the activities of caspase8 and caspase3 were significantly enhanced (P＜0.01). ConclusionThe upregulation effect of euphorbiasteroid on Fas/FasL signaling pathway might contribute to the apoptosis of HL60 cells in a dosedependent manner. References | Related Articles | Metrics

Inhibitory effect of knockingdown Yesassociated protein for the growth of SNB19 glioma cells Yu Fuhua, Jia Zhifan, Pu Peiyu, Wang Guangxiu, Zhang Anling, Yang Weidong 2014, 41 (8):  684-688.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.013 Abstract ( 439 )   PDF (1496KB) ( 1471 )   Save ObjectiveTo investigate the effect of knockingdown Yesassociated protein (YAP) on the biological characteristics of SNB19 glioblastoma cell. MethodsThe expression of YAB in SNB19 was knockdown by YAB small interfering RNA (YABsiRNA). The downregulation of YAP expression was identified by Western blot analysis. The proliferative ability of cell was determined by methyl thiazoyl terazolium (MTT). The invasive ability of cell was examined by Transwell assay. Flow cytometry and Annexin V staining were used to detect the cell cycle and apoptosis respectively. The results were analyzed by the statistical software SPSS18.0. ResultsThe expression of YAP in the cells transfected with YAPsiRNA was significantly reduced. The cell proliferation activity of SNB19 cells was inhibited, which decreased from (100.00±0.00)% to (52.32±3.10)% (F=33.00, P<0.01). The cell cycle was arrested in G0G1 phase (F=8.76, P<0.01). The cell invasive ability was attenuated apparently, which decreased from (163.20±10.10) to (37.71±2.52) (F=282.05, P<0.01). The apoptosis ratio of the tumor cell which transfected with YAPsiRNA was increased from (3.56±0.35)% to (18.99±0.66)%, (F=931.99, P<0.01). ConclusionKnockingdown YAP expression in glioma cells could inhibit the proliferative activity and invasive ability of SNB19 cell and could induce cell apoptosis. YAP could be served as a potential target for the gene therapy of glioma. References | Related Articles | Metrics

Expressions and clinical significances of DLC-1 and ROCKⅠ in nonsmall cell lung cancer Zhai Yujie, Fan Qingshuai, Ning Fangling, Liu Changmin, Zhao Dahua, Chen Shaoshui 2014, 41 (8):  688-692.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.014 Abstract ( 483 )   PDF (1083KB) ( 1418 )   Save ObjectiveTo explore the expressions and clinical significances of deleted in liver cancer1 (DLC1) and Rho associated coiledcoil forming protein kinase (ROCK)Ⅰ in nonsmall lung cancer (NSCLC). MethodsThe expressions of DLC1 and ROCKⅠ in NSCLC and adjacent tissue of 48 patients with pathologically confirmed as NSCLC and undergone surgical resection were detected by immunohistochemistry EnVision method. The correlations among DLC1 protein, ROCKⅠ protein and the clinical pathological characteristics were analyzed. The prognostic value of DLC1 in patients with NSCLC was studied. ResultsThe expression of DLC1 protein in NSCLC tissue was low or missing, and the positive rate was 33.3% (16/48), significantly lower than that in the tissue adjacent to carcinoma 70.8% (34/48), with statistical significance (χ2=13.523, P<0.01). The positive expression rate of ROCKⅠ protein in NSCLC was 58.3% (28/48), higher than that of tissue adjacent to carcinoma 0（0/48）, with statistical significance (χ2=39.529, P<0.01). The expression of DLC1 protein was correlated with tumor differentiation, lymph node metastasis and clinical stage, rather than with sex, smoking history and organization type. Through the correlation analysis, the expression of ROCKⅠ in DLC1 positive group was 37.5% (6/16), and the expression rate of ROCKⅠ in DLC1 negative group was 68.8% (22/32). There was negative correlation between DLC1 and ROCKⅠ in NSCLC tissues (r=-2.214, P=0.039). The 3 year survival rate in DLC1 protein high expression group was obviously higher than that in low expression group, with statistical significance (P=0.043). ConclusionLow or missing expression of DLC1 and high expression of ROCKⅠ protein may play an important role in the occurrence and development of NSCLC. Detecting the expression of DLC1 and ROCKⅠ protein may be useful for evaluating the biological behavior and prognosis of NSCLC. References | Related Articles | Metrics

Dosage studies on simplified inverse intensity modulated radiotherapy in patients with early-stage breast cancer after breastconserving surgery Hao Furong, Lyu Chunyan, Wang Jinpeng, Wang Peihe, Li Yong, Ma Ruizhong, Wang Mingchen 2014, 41 (8):  692-696.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.015 Abstract ( 500 )   PDF (1051KB) ( 1421 )   Save ObjectiveTo compare the dosage characteristics between threedimensional conformal radiotherapy (3DCRT) plan and simplified inverse dynamic intensity modulated radiotherapy (IMRT) in patients with earlystage breast cancer after breastconserving surgery. Methods3DCRT and IMRT treament plans were designed for 14 female patients with earlystage breast cancer after breastconserving surgery, 4 of whom were left breast cancer cases. A dose of 50 Gy in 25 fractions to the whole ipsilateral breast was delivered using 6 MV photons for 3DCRT or IMRT. For 3DCRT plans, tangential field irradiation was adopted. While for IMRT, reverse dynamic intensity modulated technology was done through two pairs of tangentiallikely fields, and 10 Gy was boosted to the tumor bed concomitantly in 25 fractions. The conformity index (CI), heterogeneity index (HI), dose and volume of organs at risk were evaluated by dose volume histograms (DVH). ResultsCompared with 3DCRT plans for ipsilateral lung, the high dose volumes were reduced and the low dose volumes were increased in IMRT plans. The same phenomenon was also observed for the heart of the patient with left breast cancer. The crosspoint doses of 3DCRT DVH and IMRT DVH for lung or heart were (25.16±9.11) Gy, (28.63±10.41) Gy respectively. There was no difference between the two plans in the V10 of contralateral breast ［IMRT(4.13±5.17)%∶3DCRT(1.99±2.43)%, t=2.11, P＞0.05］, but the D30 and mean of IMRT plan were higher than that of 3DCRT ［(2.23±1.77) Gy∶(1.20±0.46) Gy, t=2.58, P＜0.05; (2.35±1.59) Gy∶(1.54±0.88) Gy, t=3.15, P＜0.01］. The difference in HI between the two plans was not observed ［IMRT(1.25±0.10)∶3DCRT(1.23±0.11), t=1.25, P＞0.05］. While the CI of IMRT plans were improved compared with 3DCRT ［(0.75±0.07)∶(0.62±0.09), t=5.68, P＜0.000 1］. ConclusionCompared with 3DCRT plan in patients with earlystage breast cancer after breastconserving surgery, the main advantages of four fields simplified inverse dynamic IMRT are concomitant tumor boosting, decreasing the high dose volumes of ipsilateral lung, and improving the CI of planning target volume at the same time, but the HI is not improved. The IMRT plan is a simple, rational and feasible design scheme. References | Related Articles | Metrics

Expression and significance of β-catenin in gastric adenocarcinoma and  the metastasis of lymphnode Du Tianxing, Yang Jiyuan, Li Long, Zhao Huichuan, Chen Tingxuan, Li Junchuan 2014, 41 (8):  696-699.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.016 Abstract ( 531 )   PDF (1078KB) ( 1324 )   Save ObjectiveTo explore the expressions of βcatenin in gastric adenocarcinoma primary tumors and metastatic lymph nodes， and to determine if it is associated with infiltration degree and whether it can provide the basis for gastric cancer metastasis. MethodsThe expressions of βcatenin were detected in 156 patients with gastric adenocarcinoma specimens, 40 cases with the corresponding lymph nodes and 12 cases of normal gastric tissues by the method of immunohistochemistry.  The expressions of βcatenin in gastric adenocarcinoma and corresponding lymph node metastases were analysed. The relationship between the expression of βcatenin and the clinical pathological features of gastric adenocarcinoma was ascertained, and difference between them was observed. ResultsThe positive expression rate of βcatenin in normal gastric tissue membrane was 100.0%(12/12).  The expression rate of βcatenin was 29.5% (46/156) in adenocarcinoma cell membrane, and it was 10.0% (4/40) in metastatic lymph nodes cell membrane. The positive expression of βcatenin was not associated with patient′s age(χ2=2.160, P=0.142), gender (χ2=1.229, P=0.268), but it was associated with tumor infiltration degree (χ2=4.032, P=0.045), degree of tumor differentiation (χ2=6.093, P=0.048), tumor stage (χ2=4.591, P=0.032), and metastasis lymph nodes (χ2=4.485, P=0.034). The incidence of abnormal or loss expression of βcatenin in gastric adenocarcinoma metastatic lymph nodes was higer than that in gastric adenocarcinoma (χ2=6.362, P=0.012). ConclusionThe expression of βcatenin will be a great help to judge the biological behaviors such as the malignant degree of tumor and the capacity of tumor invasion and metastasis. References | Related Articles | Metrics

Expressions and clinical significances of Livin and vascular endothelial growth factor in human pancreatic carcinoma Xue Dong, Zhao Haixia, Chang Gang, Li Xinjun, Li Mengyu, Cheng Piguang, Zhang Chengde, Zhang Tongjun 2014, 41 (8):  700-703.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.017 Abstract ( 511 )   PDF (967KB) ( 1220 )   Save ObjectiveTo investigate the expressions of Livin and vascular endothelial growth factor (VEGF) in pancreatic carcinoma and their cilinical significances. MethodsThe expressions of Livin and VEGF proteins were tested by immunohistochemistry in 68 cases of pancreatic carcinomas and 44 cases of adjacent paracancerous tissues．ResultsThe positive rates of Livin in pancreatic carcinomas and adjacent paracancerous tissues were 73.5% and 4.5% respectively, and the difference was statistically significant (χ2=48.137, P＜0.001). The positive rates of VEGF in pancreatic carcinomas and adjacent paracancerous tissues were 69.1% and 13.6% respectively, and the difference was statistically significant (χ2=29.147, P＜0.001). The expressions of Livin and VEGF were related with tumor differentiation (χ2=6.061, P=0.014; χ2=6.592, P=0.010), TNM stage (χ2=4.175, P=0.041; χ2=9.992, P=0.002), lymph node metastasis (χ2=11.731, P=0.001; χ2=12.002, P=0.001) and neural invasion (χ2=9.950, P=0.002; χ2=7.433, P=0.006). Significantly positive correlation was found between the expressions of Livin and VEGF by using Spearman correlation analysis (r=0.320, P=0.008). Survival analysis showed that the expressions of Livin and VEGF were independent prognostic factors in pancreatic carcinoma. ConclusionLivin and VEGF involve in the development, migration and metastasis of pancreatic carcinoma. Livin may upregulate the expression of VEGF, which may lead to the angiogenesis and migration in pancreatic carcinoma. References | Related Articles | Metrics

Clinical significance of hepatocyte nuclear factor 4α in rectal cancer and its relationship with prognosis Wang Juan, Ji Weiping, Yao Houshan, Wang Liangzhe, Hu Zhiqian 2014, 41 (8):  704-708.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.018 Abstract ( 506 )   PDF (954KB) ( 1261 )   Save ObjectiveTo investigate the clinical significance of hepatocyte nuclear factor 4α (HNF4α) in rectal cancer and its relationship with prognosis. MethodsRealtime PCR was designed to detect the expression of HNF4α on mRNA level and the immunohistochemistry was used to determine the expression of HNF4α on protein level in rectal cancer tissue. The relationship between HNF4α expression and clinical characteristics was also analysed. The KaplanMeier method was used for univariate analysis and a Cox proportional hazards regression model was performed for multivariate analysis. ResultsHNF4α was low expressed both on mRNA (t=6.092, P＜0.001) and protein level (χ2=15.230, P＜0.001) in rectal cancer tissue. HNF4α expression on protein level was related with the clinical stage (χ2=48.311, P＜0.001), depth of invasion (χ2=23.911, P＜0.001), histological differentiation (χ2=20.787, P＜0.001), lymph node metastasis (χ2=39.064, P＜0.001) and distant metastasis (χ2=5.146, P=0.04), while age and gender were not relevant. The cumulative 3year overall survival of patients with low HNF4α expression (43.8%) was much worse than the patients with high HNF4α expression (95.5%), and the difference was statistically significant (P＜0.001). Univariate analysis revealed that HNF4α expression (χ2=28.778, P＜0.001), differentiation (χ2=26.680, P＜0.001), clinical stage (χ2=32.702, P＜0.001), depth of invasion (χ2=6.226, P=0.013), lymph node invasion (χ2=15.270, P＜0.001) and distant metastasis (χ2=21.817, P＜0.001) were statistically significant worse predictors for rectal cancer, whereas age and gender were not relevant. The multivariate Cox proportional hazard analysis revealed that HNF4α low expression (RR=6.084, P=0.028) was independent prognostic markers for 3year overall survival in the patients with rectal cancer. ConclusionHNF4α was closely related to the tumorigenesis and progression of rectal cancer, which is an independent prognostic marker for rectal cancer, and which may be an effective target for the therapy of rectal cancer. References | Related Articles | Metrics

Predictive effect of microRNA ratio in osteosarcoma Ren Huiwen, Yang Cheng, Su Hongwei, Li Hongwei 2014, 41 (8):  708-711.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.019 Abstract ( 490 )   PDF (1155KB) ( 1524 )   Save ObjectiveTo explore the roles of biomarkers of the ratios of microRNA (miRNA) in osteosarcoma. MethodsThe blood samples from 20 patients with osteosarcoma and 30 healthy people with similar age were choosed. The miRNA expression profiles were filtered by gene chips of high flux. The results were verified by qRTPCR. The miRNA expression ratio was analysed by receiver operating characteristic (ROC) curve aiming to find the most predictive potential combinationtype markers. ResultsTwo hundred and fiftyeight miRNAs were found to be significantly deregulated in blood samples from 30 patients with osteosarcoma as compared with their matching normal blood samples (F=5.564, P＜0.05). Four miRNAs were choosed from blood samples of 22 patients with osteosarcoma and normal blood samples. MiR181b, miR199b5p and miR451 were significantly higher than that in normal blood sample (F=6.283，P＜0.05), while miR124 showed low expression (F=7.201，P＜0.05). Furthermore, the ratio of miR199b5p/miR124 showed a high sensitivity (96%) and specificity (97%). ConclusionThe abnormal expression of miRNA in osteosarcoma makes it have a predictive effect. The ratio of miR199b5p/miR124 could be served as combinationtype biomarkers in the diagnosis of osteosarcoma. References | Related Articles | Metrics

Expression and significance of MYC/BCL2 protein coexpression in diffuse large B-cell lymphoma Fang Jianchen, Li Zheng, Xia Zhaoxia, Zhang Huizhi 2014, 41 (8):  712-714.  doi: 10.3760/cma.j.issn.1673-422X.2014.09.020 Abstract ( 669 )   PDF (949KB) ( 1944 )   Save ObjectiveTo explore the condition of MYC/BCL2 protein coexpression in 245 patients with diffuse large Bcell lymphoma (DLBCL) and to find the correlations among MYC/BCL2 protein coexpression, the germinal center Bcelllike (GCB) subtype and nonGCB subtype. MethodsParaffinembedded lymphoma samples from 245 patients with DLBCL were studied using immunohistochemistry for MYC, BCL2, CD10, BCL6, MUM1 and KI67. And the protein expressions of them were analyzed. ResultsIn the specimens of 245 patients with DLBCL, the patients with nonGCB were 163 (66.5%), and the patients with GCB were 82 (33.5%). The group of MYC/BCL2 protein coexpression comprised 35.9% (88/246) of the all patients. NonGCB subtype group had a significantly higher frequency of MYC/BCL2 protein coexpression than the GCB subtype group (41.7%∶24.4%; χ2=7.116, P=0.008). ConclusionThe data shows that MYC/BCL2 protein coexpression occurs significantly more commonly in the nonGCB subtype. We can predict prognosis and choose therapeutic regimen base on the assessment for MYC and BCL2 protein expression. References | Related Articles | Metrics