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08 February 2026, Volume 53 Issue 2 Previous Issue   

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Original Article

Effects of cordycepin on proliferation， apoptosis and epithelial mesenchymal transition in nasopharyngeal carcinoma cells by regulating Akt/GSK-3β/Snail signaling pathway Shi Rui, Dai Jian, Chen Ran, Hu Lili 2026, 53 (2):  65-72.  doi: 10.3760/cma.j.cn371439-20250415-00010 Abstract ( 7 )   HTML ( 0 )   PDF (2468KB) ( 3 )   Save Objective To explore the effects of cordycepin on proliferation， apoptosis and epithelial mesenchymal transition （EMT） in nasopharyngeal carcinoma cells by regulating Akt/GSK-3β/Snail signaling pathway. Methods The nasopharyngeal carcinoma CNE-1 cells were divided into low， medium， high dose cordycepin groups （5， 10， 20 μmol/L cordycepin）， high dose cordycepin+SC79 group （20 μmol/L cordycepin+10 μmol/L Akt/GSK-3β/Snail signaling pathway activator SC79） and control group， and cells were intervened for 24 h in each treatment group. Cell cloning situation was detected by plate cloning assay； cell migration was detected by cell scratch assay； Transwell chamber method was used to detect cell invasion； flow cytometry was used to detect cell apoptosis； Western blotting was used to detect the expression of Akt， p-Akt， GSK-3β， p-GSK-3β， Snail， N-cadherin， vimentin， E-cadherin， Bcl-2 and Bax proteins. Results The results of the plate cloning assay showed that the numbers of cell clones in the control group， the low， medium and high dose cordycepin groups， and the high dose cordycepin+SC79 group were 116.83±4.35， 93.17±4.01， 70.50±3.83， 52.33±3.41， and 69.17±3.29， respectively. The results of the cell scratch assay showed that the scratch healing rates of the 5 groups of cells were （38.16±2.37）%， （30.27±2.26）%， （24.19±2.01）%， （17.15±1.92）%， and （22.73±2.15）%. The results of the Transwell method showed that the numbers of cell invasions in the 5 groups were 96.50±3.49， 78.33±3.12， 62.17±2.93， 43.50±2.58， and 57.33±2.27， respectively. The results of flow cytometry showed that the apoptosis rates of the 5 groups of cells were （2.46±0.35）%， （15.93±1.59）%， （23.17±1.64）%， （29.39±1.86）%， and （24.84±1.72）%， respectively， all with statistically significant differences （F=260.21， P<0.001； F=83.58， P<0.001； F=295.35， P<0.001； F=282.13， P<0.001）. The numbers of cell clones and the numbers of cell invasions in the control group and the low， medium and high dose cordycepin groups decreased successively， and the scratch healing rates decreased successively， while the cell apoptosis rates increased successively （all P<0.05）. Compared to the high dose cordycepin group， the high dose cordycepin+SC79 group had more cell clones and cell invasions， a higher scratch healing rate， and a lower apoptosis rate （all P<0.05）. The results of Western blotting showed that the expression levels of N-cadherin protein in the control group， the low， medium and high dose cordycepin groups， and the high dose cordycepin+SC79 group were 0.82±0.08， 0.68±0.05， 0.55±0.04， 0.39±0.02 and 0.53±0.06， respectively. The expression levels of vimentin protein in the 5 groups were 0.94±0.09， 0.76±0.07， 0.59±0.06， 0.42±0.03 and 0.56±0.05， respectively. The expression levels of E-cadherin protein in the 5 groups were 0.42±0.04， 0.57±0.06， 0.73±0.07， 0.91±0.09 and 0.78±0.07， respectively. The expression levels of Bcl-2 protein in the 5 groups were 0.89±0.08， 0.73±0.06， 0.57±0.05， 0.43±0.03 and 0.55±0.07， respectively. The expression levels of Bax protein in the 5 groups were 0.31±0.03， 0.45±0.05， 0.61±0.06， 0.78±0.08 and 0.67±0.06， respectively. The expression levels of p-Akt/Akt in the 5 groups were 0.87±0.08， 0.75±0.06， 0.60±0.05， 0.46±0.03 and 0.58±0.04， respectively. The expression levels of p-GSK-3β/GSK-3β in the 5 groups were 0.98±0.09， 0.84±0.08， 0.69±0.06， 0.54±0.05 and 0.66±0.06， respectively. The expression levels of Snail protein in the 5 groups were 0.92±0.08， 0.74±0.05， 0.56±0.05， 0.37±0.03 and 0.52±0.06， respectively， all with statistically significant differences （F=54.89， P<0.001； F=60.27， P<0.001； F=47.10， P<0.001； F=52.26， P<0.001； F=60.67， P<0.001； F=50.94， P<0.001； F=36.10， P<0.001； F=85.13， P<0.001）. In the control group and the low， medium and high dose cordycepin groups， the expression levels of N-cadherin， vimentin， Bcl-2， p-Akt/Akt， p-GSK-3β/GSK-3β， and Snail protein decreased successively （all P<0.05）， while the expression levels of E-cadherin and Bax protein increased successively （all P<0.05）. Compared with the high dose cordycepin group， the expression levels of N-cadherin， vimentin， Bcl-2， p-Akt/Akt， p-GSK-3β/GSK-3β， and Snail protein in the high dose cordycepin+SC79 group were higher （all P<0.05）， while the expression levels of E-cadherin and Bax protein were lower （both P<0.05）. Conclusions Cordycepin can promote apoptosis of nasopharyngeal carcinoma cells and inhibit cell invasion， migration， proliferation and EMT， which may be related to the down-regulation of Akt/GSK-3β/Snail signaling pathway. Figures and Tables | References | Related Articles | Metrics

Effect of SPART on the proliferation and migration capabilities of gastric cancer cell through lipophagy Laibijiang Wusiman, Song Dingding, Zhang Wenbin 2026, 53 (2):  73-78.  doi: 10.3760/cma.j.cn371439-20250612-00011 Abstract ( 5 )   HTML ( 0 )   PDF (2280KB) ( 2 )   Save Objective To investigate the expression of SPART in gastric cancer tissues and its effects on lipophagy， as well as proliferation and migration in gastric cancer cells. Methods Bioinformatics analysis was performed on gastric cancer transcriptome data from the TCGA database to assess SPART expression level. Five patients diagnosed with gastric cancer and undergoing surgical treatment at the Affiliated Tumor Hospital of Xinjiang Medical University from April 2024 to April 2025 were selected， and Western blotting was used to measure SPART protein levels in gastric cancer and adjacent normal tissues. The experimental groups of human gastric cancer cell lines AGS and NUGC3 were all transfected with SPART overexpression plasmids， while the control groups were all transfected with blank vectors. Western blotting was used to detect the effects of overexpressing SPART on the expression of microtubule-associated protein light chain 3 （LC3）， lysosome-associated membrane protein 2 （LAMP2）， and perilipin 3 （PLIN3）. Cell proliferation and migration capabilities were assessed using CCK-8 and Transwell assays， respectively. Immunofluorescence was used to examine lipid droplet content and LC3 levels after SPART overexpression. Results Bioinformatics analysis revealed that the expression levels of SPART in 375 gastric cancer tissues and 32 normal gastric mucosa tissues were 3.06 （1.62） and 3.54 （2.68）， respectively， with a statistically significant difference （U=7 429.00， P=0.025）. Among the 27 pairs of paired samples， the relative expression level of SPART in gastric cancer tissues was 2.78±0.82， which was lower than that in normal gastric tissues （3.84±1.22）， with a statistically significant difference （t=-3.80， P<0.001）. Immunohistochemical staining data from the Human Protein Atlas database showed that among the 23 gastric cancer tissue samples， SPART was lowly expressed in 16 cases （69.57%）， moderately expressed in 7 cases （30.43%）， and had no high expression （0）. Western blotting analysis showed that the relative expression level of SPART protein in gastric cancer tissues in clinical samples was 0.56 （0.68）， which was lower than that in adjacent tissues 1.38 （1.05）， with a statistically significant difference （U=4.00， P=0.016）. CCK-8 assay results showed that at 48 h， the absorbance （A） values of the control and experimental groups of AGS cells were 0.52±0.01 and 0.41±0.01， respectively； those of the control and experimental groups of NUGC3 cells were 0.67±0.02 and 0.51±0.01， respectively， with statistically significant differences （t=13.47， P<0.001； t=12.39， P<0.001）. At 72 h， the A values of the control and experimental groups of AGS cells were 0.82±0.01 and 0.67±0.01， respectively； those of the control and experimental groups of NUGC3 cells were 1.05±0.01 and 0.78±0.02， respectively， with statistically significant differences （t=18.37， P<0.001； t=20.91， P<0.001）. At 96 h， the A values of the control and experimental groups of AGS cells were 1.04±0.03 and 0.74±0.01， respectively； those of the control and experimental groups of NUGC3 cells were 1.80±0.02 and 1.41±0.08， respectively， with statistically significant differences （t=16.43， P<0.001； t=8.19， P=0.004）. Transwell assay revealed that the numbers of migrating cells in the AGS and NUGC3 cell experimental groups were 500±17 and 276±21， respectively， which were less than those in the control groups （771±33 and 327±12）， with statistically significant differences （t=-12.65， P<0.001； t=-3.65， P=0.030）. Western blotting assay showed that， in the control and experimental groups of AGS cells， the expression levels of LC3 protein were 0.47±0.08 and 1.06±0.32， respectively， and the expression levels of LAMP2 protein were 0.65±0.03 and 0.98±0.11， respectively， the expression levels of PLIN3 protein were 0.82±0.15 and 0.43±0.10， respectively， all with statistically significant differences （t=3.11， P=0.040； t=5.02， P=0.007； t=3.75， P=0.020）. In the control and experimental groups of NUGC3 cells， the expression levels of LC3 protein were 0.89±0.04 and 1.45±0.16， respectively， and the expression levels of LAMP2 protein were 0.68±0.18 and 1.22±0.22， respectively， the expression levels of PLIN3 protein were 1.71±0.45 and 0.35±0.06， respectively， all with statistically significant differences （t=5.89， P=0.004； t=3.29， P=0.030； t=5.19， P=0.007）. Immunofluorescence assay showed that， the lipid droplet levels in the AGS cell control group and the experimental group were 1.08±0.09 and 0.32±0.03， respectively， and the LC3 levels were 0.40±0.01 and 0.88±0.15， respectively， with statistically significant differences （t=13.88， P<0.001； t=-5.53， P=0.033）. Conclusions SPART is lowly expressed in gastric cancer， and its overexpression can inhibit gastric cancer cell proliferation and migration while activating lipophagy. Figures and Tables | References | Related Articles | Metrics

Predictive value of multimodal ultrasound radiomics features for lymphovascular invasion in patients with papillary thyroid cancer Liu Juan, Xu Lei, Wan Jing, Lyu Juan, Zhang Li 2026, 53 (2):  79-86.  doi: 10.3760/cma.j.cn371439-20250627-00012 Abstract ( 3 )   HTML ( 0 )   PDF (2438KB) ( 0 )   Save Objective To explore the predictive value of multimodal ultrasound radiomics features for lymphovascular invasion （LVI） in papillary thyroid cancer （PTC）. Methods A total of 131 patients with PTC who underwent multimodal ultrasound examination and were confirmed by surgical pathology at People's Hospital of Xinjiang Uygur Autonomous Region from June 2024 to March 2025 were retrospectively collected. Patients were randomly divided into a training set （n=92） and a validation set （n=39） at a ratio of 7∶3. The training set was further divided into an LVI group （n=23） and a non-LVI group （n=69） according to whether the patients had LVI. Multivariate logistic regression analysis was used to identify factors influencing LVI and construct a clinical model. The least absolute shrinkage and selection operator （LASSO） algorithm was applied to screen radiomics features from different ultrasound modalities and develop a multimodal ultrasound radiomics score （Radscore） model. Four deep learning models were constructed using four machine learning algorithms with the selected radiomics features. A fusion model was developed by integrating logistic regression analysis. Model performance was evaluated using receiver operator characteristic （ROC） curves， calibration curves， and clinical decision curve. Results Statistically significant differences were observed between LVI and non-LVI groups in maximum tumor diameter （t=3.30， P=0.001）， tumor multifocality （χ2=17.97， P<0.001）， enhancement rate （χ2=5.69， P=0.017）， and wash-in perfusion index （WiPI） （t=2.69， P=0.009）. The results of the multivariate analysis showed that， maximum tumor diameter （OR=1.26， 95%CI：1.09-1.47， P=0.002）， WiPI （OR=0.87， 95%CI：0.78-0.97， P=0.009） and tumor multifocality （OR=6.05， 95%CI：1.97-18.60， P=0.002） were all independent influencing factors for the occurrence of LVI in patients with PTC. After dimensionality reduction via LASSO regression， 6 radiomic features for conventional ultrasound and 11 radiomic features for contrast-enhanced ultrasound were obtained. A multimodal ultrasound model was constructed based on the aforementioned features：logit（P）=-0.27+0.17×conventional ultrasound+0.30×contrast-enhanced ultrasound. The multimodal ultrasound Radscores of the LVI group patients in the training set and validation set （3.38±1.20， 4.02±1.45） were significantly higher than those of the non-LVI patients （1.76±0.83， 1.40±0.54）， with statistically significant differences （t=7.20， P<0.001； t=8.28， P<0.001）. Among the 4 constructed deep learning models， the support vector machine model exhibited the best performance and could be used for subsequent studies. ROC curve analysis showed that the fusion model， which was constructed by combining the clinical model， multimodal ultrasound radiomics model， and deep learning model， had an area under the curve of 0.90 （95%CI：0.81-0.95） for diagnosing LVI， representing the optimal diagnostic performance. Calibration curve analysis indicated that the predictive probabilities of the fusion model showed good agreement with the actual probabilities and closely approached the ideal curve. Decision curve analysis showed that the fusion model had the highest net benefit. Conclusions Multimodal ultrasound radiomics models exhibit good predictive performance for LVI in PTC patients. The fusion model combining multimodal ultrasound radiomics， clinical features， and deep learning can improve diagnostic efficiency， demonstrating high clinical application value. This approach shows certain calibration abilities and is expected to provide an objective basis for precise and personalized clinical treatment. Figures and Tables | References | Related Articles | Metrics

Analysis of the correlation between nutritional status and preoperative gastric morphology and functional characteristics in patients with gastric cancer after radical gastrectomy Arya Ehmet, Nuriman Samat, Wang Tingting 2026, 53 (2):  87-92.  doi: 10.3760/cma.j.cn371439-20250607-00013 Abstract ( 4 )   HTML ( 0 )   PDF (802KB) ( 0 )   Save Objective To explore the correlation between the nutritional status of patients and preoperative gastric morphology and functional characteristics in patients with gastric cancer after radical gastrectomy. Methods From September 2021 to September 2024， 134 patients with stage Ⅰ-Ⅱ gastric cancer who underwent radical gastrectomy （distal subtotal gastrectomy） at the First Affiliated Hospital of Xinjiang Medical University were selected as the research subjects. Clinical data of patients （including gastric morphology and functional characteristics） were collected and patients were divided into the outflow group （n=51） and the retention group （n=83） based on the preoperative gastric morphology. The related indicators of body composition analysis were collected one week before the surgery and one month after the surgery. Multiple linear regression analysis was used to analyze the related influencing factors of nutritional indicators. The occurrence of dumping syndrome， diarrhea and gastroesophageal reflux within 7 days after the surgery was statistically analyzed and compared between the two groups. The decline of nutritional indicators in patients with different complications was also compared. Results One week before surgery， the body mass index （BMI） values of patients in the outflow group and the retention group were （21.29±1.21） and （21.09±1.36） kg/m2， the fat-free BMI values were （15.29±1.07） and （15.31±1.08） kg/m2 ， the fat indexes were （5.98±0.92） and （5.84±0.83） kg/m2， the skeletal muscle indexes were （10.13±1.02） and （10.23±1.98） kg/m2， and the inorganic salt indexes were （5.01±0.98） and （4.94±0.81） kg/m2， with no statistically significant differences （all P>0.05）. One month after surgery， the BMI values of patients in the two groups were （20.75±1.82） and （20.25±1.24） kg/m2， the fat-free BMI values were （14.68±0.94） and （14.36±0.88） kg/m2 ，the fat indexes were （5.77±0.93） and （5.58±0.72） kg/m2， the skeletal muscle indexes were （9.51±1.12） and （9.46±1.57） kg/m2， and the inorganic salt indexes were （5.00±0.89） and （4.99±0.73） kg/m2， with no statistically significant differences （all P>0.05）. The fat-free BMI value and skeletal muscle index of the patients in the outflow group one week before the surgery were both higher than those one month after the surgery （both P<0.05）. The BMI value， fat-free BMI value， fat index and skeletal muscle index of the patients in the retention group one week before the surgery were all higher than those one month after the surgery （all P<0.05）. The BMI decrease rates of the two groups of patients one month after the surgery were （3.62±0.49）% and （3.94±0.48）%， respectively， with a statistically significant difference （t=3.67， P<0.001）. The decrease rates of fat-free BMI were （3.75±0.51）% and （4.06±0.47）%， respectively， with a statistically significant difference （t=3.52， P<0.001）. The rates of decrease in fat index were （3.54±0.43）% and （3.82±0.49）%， respectively， with a statistically significant difference （t=3.47， P<0.001）. The decrease rates of skeletal muscle index were （3.76±0.53）% and （4.01±0.48）%， respectively， with a statistically significant difference （t=2.75， P=0.007）. The decrease rates of inorganic salt index were （0.14±0.05）% and （0.13±0.04）%， respectively， with no statistically significant difference （t=1.21， P=0.229）. The analysis results of the related influencing factors of nutritional indicators showed that， gastric morphological classification was independently correlated with the decrease rate of fat index （t=-2.90， P=0.005） and the decrease rate of skeletal muscle index （t=-4.50， P<0.001）， and serum albumin （t=2.01， P=0.047） was independently correlated with the decrease rate of skeletal muscle index. Within 7 days after the surgery， the total incidence of complications in the retention group was 36.14% （30/83）， and that in the outflow group was 15.69% （8/51）， with a statistically significant difference （χ2=6.51， P=0.011）. The rates of decrease in fat index and skeletal muscle index in patients with dumping syndrome were （4.21±0.79）% and （4.48±0.94）%， respectively， while those in patients without dumping syndrome were （3.61±0.47）% and （3.84±0.43）%， respectively， with statistically significant differences （t=2.59， P=0.025； t=2.33， P=0.039）. The rates of decrease in fat index and skeletal muscle index in patients with diarrhea were （4.24±0.81）% and （4.41±0.85）%， respectively， while those in patients without diarrhea were （3.62±0.43）% and （3.85±0.41）%， respectively， with statistically significant differences （t=2.62， P=0.024； t=2.26， P=0.045）. The rates of decrease in fat index and skeletal muscle index in patients with gastroesophageal reflux were （4.19±0.72）% and （4.45±0.89）%， respectively， while those in patients without gastroesophageal reflux were （3.64±0.39）% and （3.82±0.43）% ， respectively， with statistically significant differences （t=2.61， P=0.024； t=2.42， P=0.037）. Conclusions The preoperative gastric morphology and functional characteristics have a significant influence on the nutritional status of patients with gastric cancer after distal subtotal gastrectomy， and are also closely related to the occurrence of complications such as dumping syndrome， diarrhea and gastroesophageal reflux within 7 days after surgery. Figures and Tables | References | Related Articles | Metrics

Analysis of the association between plasma D-dimer levels and thromboembolic risk in patients with malignant solid tumors Lin Xueqiong, Chen Ting, Huang Xuchun, Wu Wenzhi, Peng Yuhui 2026, 53 (2):  93-99.  doi: 10.3760/cma.j.cn371439-20250806-00014 Abstract ( 4 )   HTML ( 0 )   PDF (1048KB) ( 0 )   Save Objective To evaluate the risk stratification value of plasma D-dimer （D-D） levels for venous thromboembolism （VTE） within 6 months in patients with malignant solid tumors. Methods A total of 11 082 patients with pathologically confirmed malignant solid tumors who were first treated at Cancer Hospital of Shantou University Medical College from January 2021 to December 2023 were retrospectively included， 1 222 cases among which completed imaging examinations such as vascular ultrasound， lower-limb venous ultrasound， or CT pulmonary angiography （CTPA） within 6 months. After excluding 95 cases with missing data， 1 127 patients were divided into the VTE group （n=338） and the non-VTE group （n=789） based on the VET diagnosis results. Gender， age， tumor location， baseline D-D measured at first visit （D-D1）， and D-D obtained on or the day before imaging （D-D2） were compared between the two groups. Multivariate logistic regression was used to analyze the independent association between plasma D-D levels and VTE. Based on the results of the multivariate analysis， the “boot” package in R was employed to perform 1 000 Bootstrap resampling iterations. In each iteration， 70% of the samples were randomly selected as the training set for constructing the prediction model， while the remaining 30% served as the validation set. Logistic regression was adopted for model construction， and a nomogram was generated using the R package “nomogram”. The receiver operator characteristic （ROC） curve was constructed to evaluate its diagnostic efficacy. Results There was a statistically significant difference in D-D1 levels between patients of different genders （Z=-5.83， P<0.001）. There were statistically significant differences in the levels of D-D1 and D-D2 in patients of different ages （χ2=585.52， P<0.001； χ2=58.56， P<0.001） and different tumor locations （χ2=1 051.12， P<0.001；χ2=227.64， P<0.001）. There were statistically significant differences in age and tumor location between the VTE group and the non-VTE group （t=-3.70， P<0.001； χ2=3 431.24， P<0.001）. The levels of D-D1 and D-D2 were significantly higher in the VTE group than those in the non-VTE group （Z=9.80， P<0.001； Z=17.12， P<0.001）. Multivariate analysis demonstrated that gender （female：OR=1.87， 95%CI：1.20-2.90， P=0.006）， age （61-70 years old：OR=0.56， 95%CI：0.32-0.98， P=0.042）， tumor location （esophagus：OR=0.30， 95%CI：0.14-0.67， P=0.003； gastrointestinal tract：OR=0.31， 95%CI：0.15-0.68， P=0.003； breast：OR=0.15， 95%CI：0.07-0.33， P<0.001； urinary tract：OR=0.33， 95%CI：0.13-0.86， P=0.023）， and D-D2 levels [551-1 100 μg/L fibrinogen equivalent units （FEU） （OR=2.55， 95%CI：1.31-4.99， P=0.006）， 1 101-4 000 μg/L FEU （OR=9.17， 95%CI：5.06-16.61， P<0.001）， and ≥4 001 μg/L FEU （OR=21.09， 95%CI：11.38-39.08， P<0.001）] were independent influencing factors for VTE in patients with malignant solid tumors. The risk of VTE increased with rising D-D2 levels. A multivariate nomogram was constructed to predict the risk of VET occurrence in patients with malignant solid tumors based on gender， age， tumor location， and D-D2 level. A D-D2 four-tier nomogram was constructed to predict the risk of VET occurrence in patients with malignant solid tumors based on the D-D2 four-tier stratification. ROC curve analysis showed that in the training set， the area under the curve （AUC） of the multivariate nomogram model for predicting VET in patients with malignant solid tumors was 0.828 （95%CI：0.798-0.858）， while the AUC of the D-D2 four‑stratification model （using 1 101-4 000 μg/L FEU as the optimal cutoff interval） was 0.811 （95%CI：0.781-0.840）. The predictive performance of the multivariate model was superior to that of the D-D2 four‑stratification model （Z=3.74， P<0.001）. In the test set， the AUC of the multivariate nomogram model for predicting VET in patients with malignant solid tumors was 0.814 （95%CI：0.763-0.864）， and that of the D-D2 four-stratification model was 0.787 （95%CI：0.733-0.841）， with no statistically significant difference （Z= 1.90， P=0.057）. Conclusions Elevated D-D is an independent risk factor for VTE within 6 months in malignant solid tumor patients. A threshold of ≥4 001 µg/L FEU can trigger intensive thrombotic work-up， facilitating early identification of high-risk patients and improving prognosis. Figures and Tables | References | Related Articles | Metrics

Review

Mechanisms of invadopodia in tumor metastasis and frontiers in therapeutic translation Zhang Long, Li Jianzhen, Zhang Wei 2026, 53 (2):  100-104.  doi: 10.3760/cma.j.cn371439-20251009-00015 Abstract ( 7 )   HTML ( 1 )   PDF (789KB) ( 1 )   Save Invadopodia are core subcellular structures in which tumor cells degrade extracellular matrix， drive local infiltration and distant metastasis. Recent studies have not only analyzed the molecular composition and dynamic assembly mechanisms， but also gradually revealed their central role as hubs in integrating tumor microenvironment signals and mediating regulatory heterogeneity across cancer types. It is noteworthy that therapeutic interventions such as radiotherapy or chemotherapy may inadvertently induce the activation of invadopodia， thus contributing to the development of tumor adaptive resistance. In response， multi-dimensional intervention strategies targeting invadopodial scaffold proteins， protease delivery systems， and extracellular vesicle-mediated communication are gaining momentum. Coupled with drug repositioning and precision nanodelivery technologies， these approaches can provide important theoretical basis and translation direction for the development of next-generation anti-metastatic therapies. References | Related Articles | Metrics

Research progress of molecular targeted therapy of ROS1 gene mutation in non-small cell lung cancer Zhao Yue, Song Chenchen, Liang Tianci, Wang Hui, Wen Tingzhi, Rong Biaoxue 2026, 53 (2):  105-110.  doi: 10.3760/cma.j.cn371439-20250810-00016 Abstract ( 5 )   HTML ( 0 )   PDF (835KB) ( 2 )   Save The incidence of ROS1 gene rearrangement in non-small cell lung cancer （NSCLC） is 1%-2%. It is involved in the proliferation， metastasis and invasion of NSCLC cells. The advent of ROS1-tyrosine kinase inhibitors （TKIs） has significantly improved the prognosis of ROS1 gene mutant NSCLC patients. However， most patients have acquired resistance after continuous drug use， which seriously challenges the therapeutic effect of tumor targeted drugs. The molecular resistance mechanism of ROS1-TKIs is not completely understood. Further exploration of the background， mutation mode， ROS1-TKIs and resistance mechanism of ROS1 fusion gene can provide new treatment ideas for NSCLC patients resistant to ROS1-TKIs. References | Related Articles | Metrics

Research progress on the impact of fibrinogen/prealbumin ratio for the prediction of prognosis of digestive system tumors Yang Rui, Guo Xinwei, Liu Yangchen 2026, 53 (2):  111-114.  doi: 10.3760/cma.j.cn371439-20250612-00017 Abstract ( 4 )   HTML ( 0 )   PDF (761KB) ( 0 )   Save Digestive system tumors have become a major global health concern， and prognosis prediction remains a focus of clinical research. The fibrinogen to prealbumin ratio （FPR）， as a novel biomarker reflecting inflammatory and nutritional status， has demonstrated significant clinical value in the prognosis prediction of digestive system tumors in recent years. Future research can focus on the combined application of FPR with other inflammatory and nutritional indicators to further improve the accuracy of prognosis prediction for patients with digestive system tumors. References | Related Articles | Metrics

Research progress and clinical significance of MET gene and its expression in ovarian cancer Cui Feifei, Huang Wenting 2026, 53 (2):  115-119.  doi: 10.3760/cma.j.cn371439-20250727-00018 Abstract ( 6 )   HTML ( 2 )   PDF (788KB) ( 1 )   Save Ovarian cancer is a common malignant tumor of the female reproductive system， which is difficult to treat and has a poor prognosis. The mesenchymal-epithelial transition factor （MET） gene and its encoded receptor tyrosine kinase c-MET play a critical role in the occurrence， development， metastasis and drug resistance of ovarian cancer. Studies have shown that MET is abnormally overexpressed or amplified in ovarian cancer， and its expression level is closely related to tumor subtype， stage， prognosis and treatment response. Overactivation of c-MET and its downstream signaling pathways not only promote tumor cell proliferation， invasion and metastasis， but also participate in the mechanisms of chemoresistance. As molecular detection technologies and targeted therapies continue to advance， MET is emerging as a significant prognostic biomarker and therapeutic target in ovarian cancer. Systematically expounding the expression characteristics， classification differences， functional mechanisms and clinical significance of the MET gene in ovarian cancer has important guiding significance for its future research directions and clinical application prospects. References | Related Articles | Metrics