Abstract: ObjectiveTo explore the enhancement effects and mechanisms of IL32β on human cervical carcinoma cells C33A to hypoxic/hypoglycemic condition. MethodsAfter cultured in hypoxia/hypoglycemic circumstance and normal circumstance for 20 hours respectively, the mRNA and protein expression of IL32β in C33A cells were detected by real timepolymerase chain reaction (RTPCR) and Western blotting respectively. Trypan blue stain was used to detect C33A cells viability in hypoxia/hypoglycemic circumstance and adding 10, 100, 500 ng/ml IL32β circumstance. The xenografted tumor of nude mice was established by intraperitoneal injection, and their volumes were tested for a given time after injecting 0, 1.0 mg/kg IL32β. siRNA was used to construct IL32β knockdown cells and detect the expression of VEGF. ResultsUnder the hypoxia/hypoglycemic circumstance, the expressions of IL32β mRNA were (6.12±0.03) times of the normal circumstance (F=43.16, P＜0.05), the expressions of IL32β protein were (2.23±0.04) times of the normal circumstance (F=22.32, P＜0.05). The C33A cells viability in hypoxia/hypoglycemic circumstance was (51.92±3.41)%, whereas, viability in 10 ng/ml IL32β group was （55.23±3.92）% (F=14.25, P＜0.05), viability in 100 ng/ml IL32β group was (62.52±4.14)% (F=35.53, P＜0.01), viability in 500 ng/ml IL32β group was (69.14±2.45)% (F=56.28, P＜0.01). After 28 days, the volume of xenografted tumor of 0 mg/kg IL32β group was (578±64)mm3, and 1.0 mg/kg IL32β group up to (1 402±142)mm3 (F=27.84, P＜0.01). In addition, compared with control group, the expression of VEGF in IL32β knockdown C33A cells was significantly decreased (F=36.85, P＜0.05). ConclusionIL32β can enhance the resistance to hypoxic/hypoglycemic condition of C33A cells, which is associated with the increase of VEGF.

Key words: Uterine cervical neoplasms, Interleukins, Vascular endothelial growth factors, Hypoxic/hypoglycemic