Y盒结合蛋白1与上皮-间质转化标志物在结直肠癌中表达的研究

doi:10.3760/cma.j.issn.1673-422X.2014.08.020

摘要/Abstract

摘要： 目的检测结直肠癌组织中Y盒结合蛋白1与上皮间质转化标志物（E钙黏蛋白和N钙黏蛋白）的表达，分析Y盒结合蛋白1与临床病理参数之间的关系并评价其与E钙黏蛋白、N钙黏蛋白之间的相关性。方法用蛋白免疫印迹和免疫组织化学染色法检测120例结直肠癌组织和相对应的癌旁正常组织中Y盒结合蛋白1、E钙黏蛋白和N钙黏蛋白表达情况，并对其结果进行分析。结果结直肠癌组织中Y盒结合蛋白1、E钙黏蛋白和N钙黏蛋白表达情况与癌旁正常组织相比差异有统计学意义（χ2=47.373，P＜0.05；χ2=83.145，P＜0.05；χ2=41.832，P＜0.05）。结直肠癌组织中Y盒结合蛋白1表达情况与肿瘤分化程度、肿瘤侵犯、淋巴结转移及远处转移有关（χ2=8.077，P=0.008；χ2=8.178，P=0.006；χ2=15.152，P＜0.001；χ2=7.368，P=0.011），与E钙黏蛋白表达呈负相关（r=-0.238，P=0.009），与N钙黏蛋白表达呈正相关（r=0.361，P＜0.001）。结论Y盒结合蛋白1可能通过参与上皮间质转化促进结直肠癌的发生发展。

关键词: Y盒结合蛋白质1, 结直肠肿瘤, 上皮-间质转化

Abstract: ObjectiveTo detect the expressions of Yboxbinding protein1 (YB1) and epithelialmesenchymal transition (EMT) markers (Ecadherin and Ncadherin) in colorectal cancer (CRC), to analyze the relationship between the expression of YB1 and clinicopathological parameters, to evaluate the correlations among YB1, Ecadherin and Ncadherin. MethodsThe expressions of YB1, Ecadherin and Ncadherin in 120 primary CRC tumors and corresponding normal tissues were detected by western blot and immunohistochemistry and the results were analyzed. ResultsThe expressions of YB1, Ecadherin and Ncadherin in tumors were significantly different from those in corresponding normal tissues (χ2=47.373, P＜0.05; χ2=83.145, P＜0.05;χ2=41.832, P＜0.05). The expression of YB1 in tumors was associated significantly with tumor differentiation, tumor invasion, lymph node metastasis and distance metastasis (χ2=8.077, P=0.008; χ2=8.178, P=0.006; χ2=15.152, P＜0.001; χ2=7.368, P=0.011). It was negatively correlated with Ecadherin expression (r=-0.238, P=0.009), but positively correlated with Ncadherin expression (r=0.361, P＜0.001). ConclusionYB1 may promote the occurrence and development of CRC by participating in EMT program.

Key words: Y-box-binding protein 1, Colorectal neoplasms, Epithelial-mesenchymal transition

严雪冰, 闫磊磊, 刘四红, 单泽志, 金志明. Y盒结合蛋白1与上皮-间质转化标志物在结直肠癌中表达的研究[J]. 国际肿瘤学杂志, 2014, 41(8): 624-628.

Yan Xuebing, Yan Leilei, Liu Sihong, Shan Zezhi, Jin Zhiming. Expression of Y-box-binding protein-1 and epithelialmesenchymal transition markers in colorectal cancer[J]. Journal of International Oncology, 2014, 41(8): 624-628.

参考文献

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