国际肿瘤学杂志

国际肿瘤学杂志 ›› 2014, Vol. 41 ›› Issue (8): 628-632.doi: 10.3760/cma.j.issn.1673-422X.2014.08.021

• 论著 • 上一篇    下一篇

DCC基因对人结直肠癌细胞株SW1116生物学行为的影响

姜洪伟, 王举, 李海军, 彭际奎, 高小平, 陈峰   

  1. 010017  呼和浩特，内蒙古自治区人民医院胃肠外科
  • 收稿日期:2014-01-02 修回日期:2014-04-02 出版日期:2014-08-15 发布日期:2014-08-14
  • 通讯作者: 姜洪伟，E-mail: jhww2009@163.com E-mail:jhww2009@163.com
  • 基金资助:

    内蒙古自治区自然科学基金（2013MS1151）

Effects of DCC gene on biological behaviors of colorectal carcinoma cell line SW1116

Jiang Hongwei, Wang Ju, Li Haijun, Peng Jikui, Gao Xiaoping, Chen Feng   

  1. Department of Gastrointestinal Surgery, Inner Mongolia People′s Hospital,  Hohhot 010017, China
  • Received:2014-01-02 Revised:2014-04-02 Online:2014-08-15 Published:2014-08-14
  • Contact: Jiang Hongwei E-mail:jhww2009@163.com

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摘要： 目的研究外源性DCC基因稳定转染对人结直肠癌SW1116细胞株的作用。方法采用反转录聚合酶链反应从人正常结肠组织中扩增DCC基因功能区片段，构建pcDNA3.1(+)DCC重组质粒并鉴定。将重组质粒转染入DCC基因缺失的结直肠癌SW1116细胞中，用四甲基偶氮唑蓝（MTT）比色法观察其抑制结直肠癌细胞SW1116的作用；用免疫荧光方法研究pcDNA3.1(+)DCC质粒转染人结直肠癌细胞SW1116后对结直肠癌细胞的影响及癌胚抗原（CEA）表达。结果转染pcDNA3.1(+)DCC质粒的SW1116细胞在转染后第3～6天，其细胞数显著低于转染pcDNA3.1(+)质粒的SW1116细胞和正常细胞对照（t=3.645，P＜0.05；t=3.132，P＜0.05）;转染pcDNA3.1(+)DCC质粒的SW1116细胞生长增殖速度低于转染pcDNA3.1(+)质粒的SW1116细胞和正常细胞对照（t=2.134，P＜0.05；t=2.736，P＜0.05）。转染pcDNA3.1(+)DCC质粒的SW1116细胞在转染后第2～6天，其总细胞活力显著低于正常细胞对照（t=3.053，P＜0.05）；转染pcDNA3.1(+)DCC质粒的SW1116细胞在转染后第2、4、5、6天，其总细胞活力显著低于转染空质粒对照（t=2.816，P＜0.05）。转染pcDNA3.1(+)DCC质粒的SW1116细胞呈黄绿色荧光的细胞数量和荧光强度明显低于转染pcDNA3.1(+)质粒的SW1116细胞和对照组细胞。结论转染DCC基因能抑制结直肠癌细胞的生长增殖，降低CEA表达，从而降低结直肠癌细胞浸润转移能力。

关键词: 结直肠肿瘤, 转染, DCC基因

Abstract: ObjectiveTo investigate the effects of exogenous wild DCC gene stably transfection on growth of colorectal carcinoma cell line SW1116 in vitro. MethodsDCC gene domain was amplified from human normal colon tissue by reverse transcriptpolymerase chain reaction (RTPCR). At first, a recombinant expression plasmid pcDNA3.1(+)DCC was constructed. Human colorectal carcinoma cell line SW1116 without DCC gene was transfected with pcDNA3.1DCC. Cell viability was tested by methyl thiazolyl tetrazolium (MTT) assay. Immunofluorescence staining was used to determine the effects of pcDNA3.1DCC and expression of carcinoembryonic antigen (CEA) in human colorectal carcinoma cell line SW1116 which was transfected with pcDNA3.1DCC. ResultsThe population of cells transfected with pcDNA3.1(+)DCC plasmid was lower than those with pcDNA3.1(+)DCC plasmid and normal cells (t=3.645, P＜0.05; t=3.132, P＜0.05) at 3～6 days after transfection, and the proliferation rate of cells transfected with pcDNA3.1(+)DCC plasmid was lower than those with pcDNA3.1(+) plasmid and normal cells (t=2.134, P＜0.05; t=2.736, P＜0.05). Cell line SW1116 transfected with pcDNA3.1(+)DCC plasmid total viability was lower than normal cells (t=3.053 ,P＜0.05) at 2～6 days after transfection. Cell line SW1116 transfected with pcDNA3.1(+)DCC plasmid total viability was lower than those with pcDNA3.1(+) plasmid (t=2.816, P＜0.05) at 2, 4, 5, 6 days after transfection. The population of flavogreen colour cells transfected with pcDNA3.1(+)DCC plasmid and the fluorescent intensity of these cells were lower than those with pcDNA3.1(+) plasmid and normal control cells. ConclusionTransfected DCC gene can suppress the cell proliferation and make CEA expression of cell line SW1116 down regulation to weaken its infiltration and metastasis abilities.

Key words: Colorectal neoplasms, Transfection, DCC gene