Study of signal transduction mechanisms of Xray induced multidrug resistance in nasopharyngeal squamous cell carcinoma CNE1 cells

doi:10.3760/cma.j.issn.1673-422X.2017.06.001

Abstract

Abstract: ObjectiveTo investigate the relationship between multidrug resistance of Xray irradiated human nasopharyngeal squamous carcinoma cell line CNE1 and platelet endothelial cell adhesion molecule1 (PECMA1)/phosphatidylinositol 3kinase (PI3K)/protein kinase B (AKT)/Bcl2 signaling pathway. Methods①The CNE1 cells were cultured in incubator. Exponentially growing CNE1 cells were exposed to 50 Gy ionizing radiation administered in 25 fractions, 2 Gy per fraction, once every two days, and the CNE1/R cells were collected. ②CNE1/R cells were cultured for 24 h, and divided into three groups: the control group, experimental group one (CNE1/R cells were processed through 1∶600 PECAM1 antibody for 24 h) and experimental group two (CNE1/R cells were processed through 10 μmol/L PI3K inhibitor LY294002 for 24 h). ③The half maximal inhibitory concentration (IC50) values of the control group and two experimental groups of CNE1/R cells were detected by methyl thiazolyl tetrazolium (MTT). ④The mRNA expressions of PI3K and Bcl2 in the control group and experimental group one were measured by semiquantitative reverse transcriptionpolymerase chain reaction (RTPCR). ⑤The mRNA expressions of AKT and Bcl2 in the control group and experimental group two were measured by semiquantitative RTPCR. Results①The IC50 of the control group, experimental group one and two were (2.99±0.02)μg/ml, (1.50±0.03)μg/ml and (1.60±0.05)μg/ml, and the difference among the three groups was statiatically signficant （F=4 381.263， P=0.000）. The IC50 of the two experimental groups were obviously lower than that of the control group (t=116.885, P=0.000; t=73.006, P=0.000). ②The semiquantitative values of PI3K in the control group and experimental group one were 1.66±0.01 and 0.90±0.05 (t=50.394, P=0.000), and the semiquantitative values of Bcl2 were 1.66±0.02 and 0.71±0.05 (t=183.165, P=0.000), and the differences were statistically significant. ③The semiquantitative values of AKT of control group and experimental group two were 1.53±0.01 and 0.72±0.01 (t=135.281, P=0.000), and the semiquantitative values of Bcl2 were 1.66±0.02 and 0.63±0.02 (t=128.814, P=0.000), and the differences were statistically significant. ConclusionPECAM1 induced multidrug resistance of human nasopharyngeal squamous cell carcinoma CNE1 cells may be related to the activity of Bcl2 through the signal pathway of PI3K/AKT.

Key words: Nasopharyngeal neoplasms, Drug resistance, neoplasm, Antigens, CD31, Xrays

Huang Ting, Liao Hehe, Liu Xiaodong, Wang Ruoyu. Study of signal transduction mechanisms of Xray induced multidrug resistance in nasopharyngeal squamous cell carcinoma CNE1 cells[J]. Journal of International Oncology, 2017, 44(6): 401-.

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