国际肿瘤学杂志

国际肿瘤学杂志 ›› 2018, Vol. 45 ›› Issue (5): 304-307.doi: 10.3760/cma.j.issn.1673422X.2018.05.012

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基于cell-SELEX的肿瘤核酸适配体筛选及其在肿瘤诊疗中的应用

王进,汪靖,廖世奇,曾家豫   

  1. 730070 兰州，西北师范大学生命科学学院（王进、汪靖、曾家豫）；甘肃省医学科学研究院医学分子中心（廖世奇）
  • 出版日期:2018-05-08 发布日期:2018-05-23
  • 通讯作者: 曾家豫,Email： zengjy@nwnu.edu.cn E-mail:zengjy@nwnu.edu.cn

Aptamers selection for tumor based on cellSELEX and its application in tumor diagnosis and treatment

Wang Jin, Wang Jing, Liao Shiqi, Zeng Jiayu   

  1. College of Life Sciences, Northwest Normal University, Lanzhou 730070, China
  • Online:2018-05-08 Published:2018-05-23
  • Contact: Zeng Jiayu E-mail:zengjy@nwnu.edu.cn

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摘要： 核酸适配体以其高特异性和高亲和力在肿瘤的早期诊断、治疗等领域显现出巨大潜能，运用cellSELEX筛选获得的核酸适配体能够通过靶分子变化识别肿瘤细胞，进而实现肿瘤的预防与治疗。目前研究者们利用cell-SELEX技术并结合流式细胞术成功筛选到多条核酸适配体。弥补适配子缺陷并发挥其优势，可使核酸适配体技术真正应用于临床诊断与治疗。

关键词: SELEX适体技术, 肿瘤

Abstract: Aptamers have shown great potential in early diagnosis, treatment of tumor because of its high specificity and affinity. The aptamers selected by cellSELEX can identify tumor cells through target molecular changes, and then realize the prevention and treatment of tumors. At present, researchers have successfully selected a number of aptamers by using cellSELEX technology combined with flow cytometry. To make up for the deficiencies and exert the advantages of aptamers can make aptamer technology really apply to clinical diagnosis and treatment.

Key words: SELEX aptamer technique, Neoplasms