国际肿瘤学杂志

国际肿瘤学杂志 ›› 2015, Vol. 42 ›› Issue (11): 817-820.doi: 10.3760/cma.j.issn.1673422X.2015.11.005

• 论著 • 上一篇    下一篇

Oct-4的表达与结直肠癌的发生及患者预后的相关性研究

周桓,胡瑜,任峰,茆勇,齐晓薇,张学光   

  1. 214062 江南大学附属医院 江苏省无锡市第四人民医院检验科（周桓、胡瑜、任峰、茆勇、齐晓薇）；苏州大学生物技术研究所（张学光）
  • 出版日期:2015-11-08 发布日期:2015-09-30
  • 通讯作者: 周桓，Email：hanson016@163.com E-mail:hanson016@163.com
  • 基金资助:

    无锡市科技发展资金项目（CSE31N1313）；无锡市医院管理中心科研项目（YGZXQ1305）

Correlation of expression of Oct4 and tumorigenesis and prognosis of colorectal cancer

Zhou Huan, Hu Yu, Ren Feng, Mao Yong, Qi Xiaowei, Zhang Xueguang.   

  1. Department of Clinical Laboratory, Forth People′s Hospital of Wuxi City， Affiliated Hospital of Jiangnan University, Jiangsu Province, Wuxi 214062, China
  • Online:2015-11-08 Published:2015-09-30
  • Contact: Zhou Huan E-mail:hanson016@163.com

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摘要： 目的研究结直肠癌演变过程中Oct4表达的变化，探讨Oct4的表达与结直肠癌患者预后的相关性。方法收集结直肠癌、配对的远端正常肠组织、结肠息肉组织，定量PCR（qRTPCR）、流式细胞术（FCM）、免疫组织化学（IHC）检测不同组织中Oct4表达水平。收集结直肠癌患者临床资料，统计学分析Oct4的表达与临床参数的相关性。KaplanMeier曲线法分析Oct4的表达与患者生存期的相关性。结果qRTPCR结果显示，Oct4 mRNA在正常组织、结肠息肉组织和恶性结直肠组织的相对表达量分别为(0.23±0.16)×10-5、(1.19±0.76)×10-5、(3.79±1.94)×10-5，组间差异具有统计学意义（F=8.633，P=0.001）。IHC检测正常组织、结肠息肉组织和恶性结直肠组织Oct4阳性率分别为4.43%、12.68%、40.51%，差异具有统计学意义（χ2=66.311，P<0.001）。Oct4的表达与结直肠癌的病理分期（χ2=7.248，P=0.007）、淋巴结转移（χ2=4.888，P=0.027）、远处转移（χ2=5.732，P=0.017）、TNM分期（χ2=4.174，P=0.041）相关。KaplanMeier生存曲线分析显示，Oct4表达阳性患者的中位生存期（37.0个月）显著短于阴性患者（76.0个月），差异具有统计学意义（χ2=14.050，P=0.001）。结论Oct4的表达在结直肠癌演变过程中逐步升高，Oct4的异常表达可能在结直肠癌演变过程中发挥一定作用，有望作为结直肠癌临床诊断、病情评估、预后判断的重要标志分子。

关键词: 结直肠肿瘤, 结肠息肉, Oct4

Abstract: ObjectiveTo study dynamic expression of Oct4 during development of colorectal cancer (CRC) and investigate the association between Oct4 expression and prognosis of patients with CRC. MethodsCRC tissues, matched nontumor tissues and colonic polyp tissues were collected and Oct4 expressions were analyzed by quantitative reverse transcriptionpolymerase chain reaction (qRTPCR), flow cytometry analysis (FCM) and immunohistochemistry (IHC). Medical records of patients with CRC were reviewed. Clinicopathological analysis was performed to assess the association between Oct4 expression and certain clinicopathological parameters. KaplaneMeier survival curve was conducted to evaluate association between Oct4 expression and survival time of patients with CRC. ResultsThe results of qRTPCR showed that the relative expressions of Oct4 mRNA in matched nontumor, colonic polyp and CRC tissues were (0.23±0.16)×10-5, (1.19±0.76)×10-5 and (3.79±1.94)×10-5 respectively, with a significant difference (F=8.633, P=0.001). IHC analysis displayed that the positive expression ratios of Oct4 in matched nontumor tissues, colonic polyp tissues and CRC tissues were 4.43%, 12.68% and 40.51% respectively, and a significant difference was discovered among them (χ2=66.311, P<0.001). Oct4 expression in CRC was significantly correlated with pathological stage (χ2=7.248, P=0.007), lymph node metastasis (χ2=4.888, P=0.027), distant metastasis (χ2=5.732, P=0.017) and TNM stage (χ2=4.174, P=0.041). KaplanMeier survival curve analysis demonstrated that Oct4 positive cases had a shorter median survival time (37.0 months) compared with Oct4 negative cases (76.0 months), with a significant difference (χ2=14.050, P=0.001). ConclusionThe expression of Oct4 is stepwise increased during development of CRC. The abnormal expression of Oct4 may play certain roles in the development of CRC. Oct4 may be an important biomarker for the clinical diagnosis, assessment of disease and prognosis in CRC.

Key words: Colorectal neoplasms, Colonic polyps, Oct4