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08 July 2016, Volume 43 Issue 7 Previous Issue    Next Issue

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Molecular mechanism of P75NTR geneinduced apoptosis in tongue squamous cell carcinoma cell Zhang-Zhao-Tao, ZHANG Feng-He, TONG Dong-Dong, LI Qing, WANG Jin-Bing, ZHANG Xin-Lian 2016, 43 (7):  481-485.  doi: 10.3760/cma.j.issn.1673422X.2016.07.001 Abstract ( 414 )   PDF (1341KB) ( 1067 )   Save Objective To investigate the molecular mechanism of P75NTR geneinduced apoptosis in tongue squamous cell carcinoma Tca8113 cell lineage. Methods P75NTR specific siRNA was transferred into P75NTR positive tongue squamous cell carcinoma Tca8113 cells.  P75NTR positive Tca8113 cells were divided into 4 groups: blank group (without transfection), negative control group (transfected with negative control siRNA), experiment group776 (transfected with siRNAP75NTR776) and experiment group1234 (transfected with siRNAP75NTR1234). Transfection efficiency and cell apoptosis were detected by flow cytometry. The interference effect of P75NTR mRNA expression was detected by fluorescence quantitative PCR. 3(4,5dimethyl2thiazoly)2,5diphenyl2Htetrazolium bromide assay was applied in measuring cell proliferation. The protein changes of P75NTR were detected by Western blotting. The distributions of nuclear factor-κB（NF-κB） of cells were observed by cell immunofluorescence labeling method. Results The transfection efficiency was 30%. The apoptosis rate of experiment group776, experiment group1234 and negative control group was (20.35±0.18) %, (12.32±1.51)% and (2.63±0.10)% respectively. Compared with the negative control group, the differences of the former two group had statistical significance (t=177.20, P<0.005; t=37.12, P<0.005). The P75NTR gene interference was successful. The inhibition rate of P75NTR protein reached 31% in experiment group776. The cell viability of Tca8113 cells after P75NTRsiRNA interference was 70.02%, 78.01% and 95.81% in experiment group776, experiment group1234 and negative control group. And there were significant differences between experiment group776 and negative control group(χ2= 235.3, P＜0.010), and  between experiment group1234 and negative control group (χ2= 117.5, P＜0.005). NF-κB distribution was increased in cell cytoplasm in the interference group than that in control group.Conclusion P75NTR may promote the proliferation or inhibit the apoptosis of tongue squamous cell carcinoma, and the molecular mechanism may be correlated with hindering the transportion of NFκB into cell nuclear. References | Related Articles | Metrics

Effect of dexmedetomidine on perioperative stress reaction and cellular immunity of patients with breast cancer radical surgery ZHOU Shao-Chun, ZHOU Wei, LI Dan, YANG Jie 2016, 43 (7):  486-489.  doi: 10.3760/cma.j.issn.1673422X.2016.07.002 Abstract ( 347 )   PDF (718KB) ( 850 )   Save ObjectiveTo study the effect of dexmedetomidine on perioperative stress reaction and postoperative cellular immunity of patients with breast cancer radical surgery. MethodsWe selected 108 cases of breast cancer patients undergoing elective radical surgery under general anesthesia in our hospital from January 2012 to June 2015. These patients were divided into dexmedetomidine group (group D) and the control group (group C) by random number table method, 54 cases in each group. Patients in group D were given dexmedetomidine 1.0 μg/kg by intravenous drip within 15 min before anesthesia and maintained 0.5 μg.kg-1.h-1 after anesthesia induction intubation until operation finish. Patients in group C were given the same capacity of normal saline instead of dexmedetomidine, and the rest of the anesthesia was same as group D. The plasma norepinephrine (NE) and epinephrine (E) and cortisol (COR), serum interleukin6 and T lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+) and NK cells levels were determined before anesthesia (T0), postoperative 4 h (T1), 1 d (T2), 3 d (T3), and 7 d (T4). ResultsThe level of plasma NE, E, COR and serum IL6 of the D group in different time had no statistical significance compared with T0 time (P﹥0.05). The plasma level of E and NE in T1T3, plasma COR in T2T3 and serum IL6 in T2T4 of the C group were increased significantly (P﹤0.05). The level of plasma E and NE in T1T3, plasma COR in T2T3 and serum IL6 in T2T4 of the D group were decreased significantly compared with C group (P﹤0.05). The level of CD3+ and NK cells in T1T3 of the two groups decreased significantly compared with T0 (P﹤0.05). The level of NK and CD3+ in T1T3 and  CD4+, CD4+/CD8+ in T2T3 of group D increased significantly compared with group C (P﹤0.05). ConclusionDexmedetomidine of intravenous drip during general anesthesia can effectively reduce stress reaction in perioperative period of breast cancer patients, and improve patients immune status. Related Articles | Metrics

Prognostic values of NLR and platelet count before and after chemotherapy in patients with advanced nonsmall cell lung cancer ZHANG Yan-Fang, XIA Jin, LI Xing-Ya 2016, 43 (7):  490-494.  doi: 10.3760/cma.j.issn.1673422X.2016.07.003 Abstract ( 512 )   PDF (722KB) ( 1331 )   Save bjectiveTo investigate the prognostic values of neutrophil to lymphocyte ratio (NLR) and platelet count before and after chemotherapy in patients with advanced nonsmall cell lung cancer (NSCLC). MethodsFrom October 2011 to December 2012, 70 patients with advanced NSCLC in Anyang Cancer Hospital of Henan Province were collected. NLR and platelet were calculated from complete blood counts in laboratory test before and after 2 cycles of chemotherapy. The patients were divided into two groups according to the NLR and platelet count: low NLR group (≤3.43) and high NLR group (>3.43); normal group (100×109/L＜platelet count＜300×109/L) and high platelet group (≥300×109/L). According to the changes of NLR before and after chemotherapy, we divided the patients into 3 groups: ①low NLR group before chemotherapy; ②high NLR group before but declined to low NLR group after chemotherapy; ③high NLR group before and after chemotherapy. According to the changes of platelet count before and after chemotherapy, we divided the patients into 3 groups: ①normal platelet count group before chemotherapy; ②high platelet count group before but declined to normal group after chemotherapy; ③high platelet count group before and after chemotherapy. The clinicopathological characteristics and longterm survivals between the subgroups were compared. ResultsThe median survival time of the low and high NLR group were 16.0 and 12.5 months, respectively, with a significant difference (χ2=3.654, P=0.041). The median survival time of the normal and high platelet group were 14.3 and 10.0 months, respectively, with a significant difference (χ2=5.358, P=0.021). The median survival times of patients in the 3 groups according to the changes of NLR before and after chemotherapy were 14.5, 12.1 and 9.0 months respectively, with statistically significant (χ2=7.701, P=0.021). The median survival times of patients in the 3 groups according to the changes of platelet count before and after chemotherapy were 14.3, 13.1 and 10.4 months, with statistically significant (χ2=12.775, P=0.002). COX multivariate analysis showed that NLR (RR=1.467, 95%CI: 1.0142.124, χ2=4.130, P=0.042)， platelet (RR=1.631, 95%CI: 1.1082.402, χ2=6.137, P=0.013) and TNM stage（RR=1.380，95%CI为1.0521.809， χ2=5.420，P=0.020） were independent prognostic factors of patients with advanced NSCLC. ConclusionNLR and platelet before chemotherapy are independent risk factors for patients with advanced NSCLC. Patients with elevated NLR and platelet have shorter survival time. References | Related Articles | Metrics

Relationship between CYP2E1 genetic polymorphism and susceptibility of gastric cancer in Hakkaness LI Tao, LAI Chun-Feng, QIU Bo, ZOU Hao-Yuan, YANG Yu-Hui 2016, 43 (7):  495-498.  doi: 10.3760/cma.j.issn.1673422X.2016.07.004 Abstract ( 558 )   PDF (896KB) ( 1189 )   Save ObjectiveThrough the research of the relationship between cytochrome P450 2E1 (CYP2E1) rs2031920 genetic polymorphism and susceptibility of gastric cancer in Hakkaness to explore the role of genetic and environmental factors in gastric cancer. MethodsA casecontrol study was performed with the molecular epidemiological methods. A total of 51 gastric cancer cases (as the gastric cancer group) and 52 healthy people (as the control group) confirmed by pathology were selected from Hakkaness in Meizhou area. The genotypes and alleles of CYP2E1 rs2031920 (C1053T) in both of the above groups were detected, and then the distribution characteristics between the two groups were analyzed. ResultsThree genotypes CC, TC and TT of rs2031920(C1053T) were found in Hakkaness in Meizhou area. The distribution frequencies of CC, TC and TT genotypes were 62.75% (32/51), 33.33% (17/51) and 3.92% (2/51) in the gastric cancer group respectively, meanwhile, 59.62% (31/52), 34.61% (18/52) and 5.77% (3/52) in control group respectively. The difference between the two groups has no statistical significance (χ2=0.235, P=0.889). The distribution frequencies of CYP2E1 rs2031920 alleles (including C and T) were 79.41% (81/102) and 20.59% (21/102) in the gastric cancer group respectively, meanwhile,76.92% (80/104) and 23.08% (24/104) in the control group respectively, which also showed no significant difference between the two groups (χ2=0.186, P=0.666). And stratified analysis such as sex, age showed no significant results (χ2=4.412, P=0.129; χ2=0.898, P=0.473). ConclusionThe genetic polymorphisms of CYP2E1 rs2031920 is possibly not related with the susceptibility of gastric cancer in Hakkaness in Meizhou area. References | Related Articles | Metrics

The expression and the diagnostic value of NOD2 in hepatocellular carcinoma ZHANG Jing-Xin, QIAO Li-Li, LIANG Ning, XIE Jian, LUO Hui, DENG Guo-Dong, ZHANG Jian-Dong 2016, 43 (7):  499-502.  doi: 10.3760/cma.j.issn.1673422X.2016.07.005 Abstract ( 604 )   PDF (1008KB) ( 1110 )   Save ObjectiveTo investigate the expression of nucleotidebinding oligomerization domain protein 2 (NOD2) in serum of patients with hepatocellular carcinoma (HCC), and to analyse the roles of NOD2 in HCC development and its clinical diagnostic value. MethodsThis study including 66 patients with HCC in the hospital from March 1, 2013 to December 31, 2014 and 61 healthy controls. Serum NOD2 levels were determined by enzymelinked immunosorbent assay (ELISA). Analysis of significance was performed with rank sum test using SPSS statistical 16.0 software. ResultsSerum levels of NOD2 in HCC patients were 171 pg/ml, significantly higher than that of healthy controls(95 pg/ml, Z=-5.00, P=0.00), and the serum NOD2 levels were correlated with clinical stage of HCC （H=56.26, P=0.00）. Compared with the serum NOD2 levels in stage Ⅰ, Ⅱ patients （106 pg/ml） and healthy controls (95 pg/ml), the serum NOD2 level in stage Ⅲ and Ⅳ (220 pg/ml) were significantly increased （χ2=31.24, P=0.00; χ2=47.23, P=0.00）, but the expression of NOD2 in stage Ⅰ and Ⅱ were nearly equal to that of the healthy controls （χ2=0.36, P=0.83）. The ROC analysis revealed that the best diagnostic cutoffpoint of serum NOD2 levels for predicting the Ⅲ and Ⅳ stages of HCC was 148.78 pg/ml, meanwhile corresponding sensitivity was 89.1% and specificity was 77.0%. Additionally, correlation analysis demonstrated that there was no significant correlation between NOD2 and alphafetal protein (r=0.44，P=0.14). Survival curves obtained that the survival time of HCC patients with NOD2 serum concentrations ≥ 200 pg/ml was significantly less than that ＜ 200 pg/ml (χ2=15.32, P<0.05). ConclusionNOD2 is highly expressed in the serum of HCC patients, especially in advanced patients, which is possibly involved in the development of HCC and has the potential to become an effective marker used for HCC diagnosis. References | Related Articles | Metrics

Correlation of peroxisome pathway reactive oxygen species oxidative stress gene and its correlation with the antitumor sensitivity of artesunate against pancreatic cancer DU Ji-Hui, ZHANG Hou-De, WEI Jing, WANG Lei, SUN Ting-Ji 2016, 43 (7):  503-507.  doi: 10.3760/cma.j.issn.1673422X.2016.07.006 Abstract ( 396 )   PDF (1061KB) ( 1506 )   Save ObjectiveTo explore the screening of peroxisome pathway reactive oxygen species (ROS) oxidative stress gene and its correlation with the antitumor sensitivity of artesunate against pancreatic cancer. MethodsBased on microarray mRNA expressions of 55 tumor cell lines in the National Cancer Institute common database, peroxisome pathwayrelated key genes which were significant correlation with halfinhibitory concentration (IC50) values of artesunate antitumor activity against human pancreatic cancer were selected by Kendall test. The candidate genes associated with artesunate sensitivity were identified and their mRNA expressions in pancreatic cancer cells were tested using fluorescent quantitative PCR. The contents of peroxidase in pancreatic cancer cells were detected through the DAB staining. ResultsThirteen key genes mRNA expressions in peroxidase pathways were significantly correlated with IC50 values for artesunate antitumor activity. Compared with normal liver cells HL7702 (1.00), CRAT (2.89±0.06), PEX11B (1.90±0.07) and PEX16 (1.35±0.07) mRNA expression levels were significantly increased in pancreatic cancer Panc1 cells which sensitive to artesunate (t=33.00, P<0.01; t=17.85, P<0.01; t=4.54, P<0.05). While CAT (1.43±0.03), SOD1 (2.07±0.04) and SOD2 (1.15±0.01) mRNA expression levels were also significantly increased in Panc1 cells which sensitive to artesunate (t=11.71, P<0.01; t=35.85, P<0.01; t=13.22, P<0.01). However, PEX12 (0.51±0.02), CAT (0.47±0.02), PRDX1 (0.43±0.01), and SOD1 (0.44±0.01) mRNA expression levels in pancreatic cancer BXPC3 cells which resistant to artesunate were significantly lower than that of HL7702 cells (t=37.53, P<0.01; t=16.52, P<0.01; t=84.20, P<0.01; t=48.24, P<0.01). DAB staining showed that the positive expression rate of peroxisomal content was apparently higher in Panc1 cells (61.5%) than that of HL7702 cells (43.8%), with a significant difference (χ2＝16.11, P<0.01). ConclusionPeroxisome and its related ROS antioxidant enzymes CAT, PRDX1, SOD gene expression may be the important factors that affect artesunate antitumor activity against human pancreatic cancer. References | Related Articles | Metrics

HERG suppresses the malignant phenotypes of osteosarcoma via modulating NF-κB pathway WU Jin, CHEN Zhi-Da, ZENG Wen-Rong, LIN Bin, WU Xin-Yu, LIU Qing-Jun 2016, 43 (7):  508-514.  doi: 10.3760/cma.j.issn.1673422X.2016.07.007 Abstract ( 457 )   PDF (2201KB) ( 1854 )   Save ObjectiveTo detect the expression of HERG (human etheràgogorelated gene)  potassium channel in human osteosarcoma, and explore the effects of silencing HERG by small interfering RNA (siRNA) on the proliferation and apoptosis of osteosarcoma cells and the mechanisms responsible for HERG regulation. MethodsThe expressions of HERG in osteosarcoma MG63 cells and tissues were detected by reverse transcription polymerase chain reaction (RTPCR), Western blotting and immunohistochemistry. Next, osteosarcoma cells were divided into three groups: HERGsiRNA group, controlsiRNA group and blank group. CCK8, colony formation, flow cytometry and Tunel assay were used to measure the proliferation and apoptosis of the osteosarcoma cells. Finally, Western blotting analysis was performed to detect the expression of nuclear factorκB (NFκB) pathway in osteosarcoma cells treated with HERG siRNA. ResultsOsteosarcoma cells and tissues were found to highly express HERG. Inhibition of HERG in the osteosarcoma cells significantly inhibited the cell proliferation and induced cell apoptosis. Compared to controlsiRNA group or blank group, HERGsiRNA could inhibit the proliferation of MG63 cells significantly ［HERGsiRNA group: (75.34±4.45)%; compared to controlsiRNA group: (100.60±5.31)%; t=3.64, P=0.007; compared to blank group: (100.00±5.66)%; t=3.43, P=0.009］. The similar results were obtained from colony formation assay (HERGsiRNA group: 134.30±11.82; compared to controlsiRNA group: 225.30±11.56; t=5.51, P=0.002; compared to blank group: 232.80±12.21; t=5.80, P=0.001). HERGsiRNA transfected MG63 cells demonstrated a significant increase of apoptotic rate compared to controlsiRNA transfected cells or untreated cells ［HERGsiRNA group: (28.10±2.21)%; compared to controlsiRNA group: (9.36±2.42)%; t=5.72, P=0.005; compared to blank group: (10.92±2.51)%; t=5.14, P=0.007］. This result was further confirmed by Tunel assay. The cells transfected with HERGsiRNA (31.57±2.08)% demonstrated extensive apoptosis, compared with the controlsiRNA group ［(10.35±1.82)%; t=7.69, P=0.002)］ or blank group ［(7.96±0.88)%; t=10.48, P=0.001］. Silencing HERG gene downregulated the cIAP1, XIAP, Bcl2, Survivin, PIκBα and NFκB p65 expression, compared to the control groups. ConclusionHERG is highly expressed in osteosarcoma. HERG silencing can suppress osteosarcoma progression through NFκB pathway and suggest that HERG may be a novel molecular target for osteosarcoma therapy and diagnosis. References | Related Articles | Metrics

Investigaion about the mRNA expression of ASB2 and Jak3 in patients with acute lymphoblastic leukemia DENG Ya-Yun, WU Wei, ZHANG Ping-An 2016, 43 (7):  515-518.  doi: 10.3760/cma.j.issn.1673422X.2016.07.008 Abstract ( 473 )   PDF (1139KB) ( 1119 )   Save ObjectiveTo explore the expression of ankyrinrepeat SOCS box containing protein 2 (ASB2) and Janus kinase 3 (Jak3) mRNA in bone marrow plasma mononuclear cells of patients with acute lymphoblastic leukemia (ALL) and discuss the correlation of them. MethodsBone marrow plasma was collected from 48 patients of newly diagnosed ALL (37 cases BALL and 11 cases TALL) and 34 non leukemia patients (control group), respectively. Expression levels of ASB2 and Jak3 mRNA were detected by realtime quantitative PCR. ResultsThe expression of ASB2 mRNA in BALL and TALL were significantly different compared to control group (t=14.2, P＜0.01; t=15.2, P＜0.01), which were 68.5, 32.7 folded more than control group, respectively. Moreover, the expression level of Jak3 mRNA in BALL group and TALL group were 2 336.1 and 7 131.5 folded more than control group (t=37.3, P＜0.01; t=37.6, P＜0.01). At last, the expression of ASB2 and Jak3 gene was positive correlation (r=0.523, P＜0.001). ConclusionASB2 and Jak3 are expressed abnormally in ALL patients and the correlation of them is positive. ASB2 and Jak3 may be related to the proliferation and differentiation in leukemia cells. References | Related Articles | Metrics

Nucleic acid aptamer for caner therapeutics and diagnostics LIANG Jian-Wei, YAN Ru-Ping, WANG Jian-Song 2016, 43 (7):  519-522.  doi: 10.3760/cma.j.issn.1673422X.2016.07.009 Abstract ( 431 )   PDF (714KB) ( 1866 )   Save ucleic acid aptamer is the systematic evolution of ligands by exponential enrichment synthesized in vitro screening technology resulting singlestranded oligonucleotides (DNA or RNA), by folding into unique spatial structure specific recognition binding target molecules. Aptamers function is similar to the antibodies but more specifically, as novel molecular probes for the field of cancer therapeutics and diagnostics. References | Related Articles | Metrics

Metronomic chemotherapy in human cancers ZHANG Bai-Hong, YUE Hong-Yun 2016, 43 (7):  523-525.  doi: 10.3760/cma.j.issn.1673422X.2016.07.010 Abstract ( 543 )   PDF (707KB) ( 1514 )   Save Metronomic chemotherapy involves antiangiogenic processes that help host to fight against cancer by using continuous low dose drugs. Metronomic chemotherapy can be an effective treatment option for patients with head and neck carcinoma, breast cancer, gastrointestinal cancer, ovarian and prostate cancer. Metronomic chemotherapy also involves multiple mechanisms that include antiangiogenesis, immune stimulation and direct tumor cell targeting effects. We need to carefully evaluate the clinical effects of combination chemotherapies that incorporate the other treatment schedules. References | Related Articles | Metrics

Progress of molecular biology and treatment for triple negative breast cancer LIU Jun-Jie, QIAN Jun 2016, 43 (7):  526-528.  doi: 10.3760/cma.j.issn.1673422X.2016.07.011 Abstract ( 405 )   PDF (706KB) ( 1359 )   Save Triple negative breast cancer (TNBC) is difficult to benefit from endocrine therapy or trastuzumab targeted drug therapy. Biological overexpression of breast cancer susceptibility gene 1, p53 gene, vascular endothelial growth factor and microRNA suggests that TNBC is easy to metastasis and recurrence and has a poor prognosis. Exploring the molecular subtypes of TNBC, setting out the treatment plan for subtypes and finding the corresponding monoclonal antibody targets are the research direction of TNBC in the future. References | Related Articles | Metrics

Endocrine therapy for postmenopausal women with hormone receptor positive in advanced breast cancer HUANG Xue-Xiang, SHI Lei 2016, 43 (7):  529-531.  doi: 10.3760/cma.j.issn.1673422X.2016.07.012 Abstract ( 451 )   PDF (709KB) ( 1395 )   Save With the emergence of new endocrine drugs and the development of research in resistance mechanisms, significant progress has been achieved in the endocrine treatment of breast cancer in recent years. Fulvestrant, the combination of       endocrine drugs and the combination of targeted therapy with endocrine therapy will be effective treatments for postmenopausal women with hormone receptor positive in advanced breast cancer. References | Related Articles | Metrics

Therapeutic effects of crizotinib in lung cancer and the treatment after drug resistance ZHU Li-Yang, YU Zhong-He 2016, 43 (7):  532-534.  doi: 10.3760/cma.j.issn.1673422X.2016.07.013 Abstract ( 439 )   PDF (707KB) ( 2113 )   Save Crizotinib is a tyrosine kinase inhibitor (TKI), which is a target for echinoderm microtubule associated protein like 4anaplastic lymphoma kinase (EML4ALK). It can prolong the progression free survival (PFS) of ALK positive patients with advanced nonsmall cell lung cancer (NSCLC). The median PFS in the firstline and secondline mPFS is 10.9 months and 7.7 months. However, despite an initial benefit, patients inevitably experience tumor progression, due to the ALK fusion gene amplification and secondary mutations of ALK kinase domain. Clinical trials show the promising efficacy like next generation ALK inhibitors and heat shock protein 90 (HSP90) can overcome acquired resistance. References | Related Articles | Metrics

Research progression of raltitrexed in the treatment of gastric cancer XUE Song, CHEN Ying-Xia 2016, 43 (7):  535-537.  doi: 10.3760/cma.j.issn.1673422X.2016.07.014 Abstract ( 665 )   PDF (709KB) ( 1419 )   Save Raltitrexed is a specific inhibitor of thymidylate synthase, and has been confirmed that raltitrexed has no cross tolerance with 5fluorouracil. Studies have shown that raltitrexedbased combination chemotherapy has a beneficial effect on the treatment of advanced gastric cancer, and well tolerance, which is worthy of clinical use. References | Related Articles | Metrics

Matrix metalloproteinase and colorectal cancer Cheng-Xin, HUANG Ying, LIU Ning 2016, 43 (7):  538-540.  doi: 10.3760/cma.j.issn.1673422X.2016.07.015 Abstract ( 334 )   PDF (704KB) ( 1126 )   Save Matrix metalloproteinase (MMP) plays an important role in the degradation of extracellular matrix components, which is crucial for tumor growth, invasion and metastasis. As one with the largest relative molecular mass in MMP family, MMP9 takes part in the generation and progression of colorectal cancer. Research shows that MMP9 can be used for the early diagnosis of colorectal cancer. The expression levels of MMP9 in tissue and plasma are negatively correlated with prognosis of the postoperative patients with colorectal cancer. In addition, targeted therapy drugs will be designed on the basis of MMP9 expression increased in colorectal cancer. References | Related Articles | Metrics

Metformin and colorectal cancer ZHAO Xiao-Tong, CHEN Ming-Wei 2016, 43 (7):  541-544.  doi: 10.3760/cma.j.issn.1673422X.2016.07.016 Abstract ( 784 )   PDF (714KB) ( 1696 )   Save Mefformin is the firstline oral medicine used to treat type 2 diabetes．Besides its glucoselowering effect, Mefformin can inhibit the proliferation of cancer cells, especially colorectal cancer cells. In recent years, there is mounting evidence that mefformin could reduce the risk and mortality of colorectal cancer, and its mechanism includes activating AMP activated protein kinase pathway, interfering with cell cycle, reducing insulin resistance, inhibiting the inflammatory response and killing colorectal cancer stem cells, etc. References | Related Articles | Metrics

Research progress of oxaliplatinbased regimen for advanced hepatocellular carcinoma SONG Di-Di, LIU Chuan-Yong 2016, 43 (7):  545-547.  doi: 10.3760/cma.j.issn.1673422X.2016.07.017 Abstract ( 433 )   PDF (708KB) ( 1266 )   Save Sorafenib is currently the only systematic molecular targeted drug approved by many countries for the treatment of advanced HCC. But sorafenib limites has survival improvement, patients appear inevitable disease progression soon. In recent years,Oxaliplatinbased regimens showed promising antitumor activity, safety and low toxicities, which provides a new way for treatment of advanced HCC. References | Related Articles | Metrics

Molecular targeted drug therapy of cervical cancer ZHOU Chun-Xia, ZHOU Xue 2016, 43 (7):  548-551.  doi: 10.3760/cma.j.issn.1673422X.2016.07.018 Abstract ( 433 )   PDF (717KB) ( 1691 )   Save Advanced and recurrent cervical cancer has a poor prognosis and few effective therapeutic option. With the development of molecular biology, the targeted drugs for patients with locally advanced cervical cancer continuously appear, such as inhibiting human papillomavirus, targeting angiogenesis, epidermal growth factor receptor, mammalian target of rapamycin, cyclooxygenase2, sarcoma gene, proteasome inhibitor, DNA methylation, etc. With the development of new molecular targeted therapy, more effective or even treatment measures of cervical cancer might be found, which can provid a new approach for the individualized treatment of cervical cancer. References | Related Articles | Metrics

Role and treatment strategy of hypoxia in the pathogenesis of multiple myeloma WANG Wei-Yuan, GUO Dong-Mei, HAN Tian-Jie, TENG Qing-Liang 2016, 43 (7):  552-554.  doi: 10.3760/cma.j.issn.1673422X.2016.07.019 Abstract ( 522 )   PDF (710KB) ( 1202 )   Save ypoxia environment plays an important role in the pathogenesis of multiple myeloma such as stimulating angiogenesis, increasing bone destructiong, epithelialmesenchymal transition and drug resistance. Thus, there are many therapeutic strategies targeting the hypoxic environment of multiple myeloma, such as hypoxiaactivated prodrug and molecular targeting inhibitors. Targeting the hypoxic environment is a promising therapy for multiple myeloma in the future. References | Related Articles | Metrics