国际肿瘤学杂志

国际肿瘤学杂志 ›› 2014, Vol. 41 ›› Issue (10): 782-785.doi: １０．３７６０／ｃｍａ．ｊ．ｉｓｓｎ．１６７３４２２Ｘ．２０１４．１０．０１９

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DMBT1基因在乳腺癌中的表达及意义

李军,陈桂林   

  1. ２７２１１１山东省济宁市第一人民医院检验科（李军）；山东
    省嘉祥县人民医院心内科（陈桂林）
  • 出版日期:2014-10-29 发布日期:2014-10-11
  • 通讯作者: 李军，Ｅｍａｉｌ：ｇｙｌｉｊｕｎ２００７＠１６３．ｃｏｍ E-mail:ｇｙｌｉｊｕｎ２００７＠１６３．ｃｏｍ

Expression of the DMBT1 gene in breast cancer and its clinical significance

 LI  Jun, CHEN  Gui-Lin   

  1. Department of Clinical Laboratory, Jining First  People′s Hospital of Shandong Province, Jining 272111, China
  • Online:2014-10-29 Published:2014-10-11
  • Contact: LI Jun E-mail:ｇｙｌｉｊｕｎ２００７＠１６３．ｃｏｍ

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摘要： 目的　探讨乳腺癌组织中 ＤＭＢＴ１基因 ｍＲＮＡ表达水平与乳腺癌临床病理特征的关系。 方法　反转录聚合酶链反应（ＲＴＰＣＲ）检测６０例乳腺癌组织、３０例乳腺癌旁增生组织和３０例乳腺癌旁 正常组织中 ＤＭＢＴ１基因 ｍＲＮＡ的表达水平，并分析其与乳腺癌临床病理学参数的关系。结果 ６０例乳腺癌组织中４４例 ＤＭＢＴ１基因 ｍＲＮＡ表达低下或完全不表达（７３．３％），１６例表达正常（２６．７％）， 癌旁正常组织和乳腺癌组织相比、癌旁增生组织与乳腺癌组织相比，差异有统计学意义（χ ２＝１１．７２，Ｐ＝ ０．０００６２；χ ２＝１５．９９，Ｐ＝０．００００６）。乳腺癌中 ＤＭＢＴ１基因 ｍＲＮＡ的低表达与患者年龄（χ ２＝１．７３３，Ｐ＝ ０．１８８）、肿瘤大小（χ ２＝０．７７６，Ｐ＝０．３７８）、组织病理分级（χ ２＝１．０００，Ｐ＝０．３１６）无显著相关。有淋巴结转 移者和临床Ⅲ期患者癌组织中 ＤＭＢＴ１基因 ｍＲＮＡ表达缺失率显著高于无转移者（χ ２＝４．８８５，Ｐ＝０．０２６） 和临床Ⅰ、Ⅱ期患者（χ ２＝４．６００，Ｐ＝０．０３２）。结论　ＤＭＢＴ１基因 ｍＲＮＡ表达降低与乳腺癌转移和临床分期 密切相关。

关键词: 乳腺肿瘤, ＤＭＢＴ１基因

Abstract: ObjectiveTo investigate the relationship between mRNA   expression of DMBT1 in breast cancer tissues and clinicopathological characteristics of patients with breast cancer. Methods60 cases of breast cancer tissues, 30 cases of breast paracancerous hyperplasia tissues and 30 cases of breast paracancerous normal tissues were selected, then reverse transcriptionpolymerase chain reaction (RTPCR) were performed to detect the differential expression of DMBT1 in mRNA levels. Furthermore, its relationship with clinicopathological characteristics of patients with breast cancer was analyzed. Results44 out of 60 breast cancer tissues were detected no or low expression of DMBT1 (73.3%), and 16 out of 60 cases were found normal expression of DMBT1 (26.7%). There were statistically significant differences between paracancerous normal tissues and breast cancer tissues, also between paracancerous hyperplasia tissues and breast cancer tissues (χ2=11.72, P=0.000 62; χ2=15.99, P=0.000 06). No significant difference was found between the low expression of DMBT1 with the age of patients (χ2=1.733, P=0.188), the size of tumor (χ2=0.776, P=0.378) and the histological grade of tissues (χ2=1.000, P=0.316). However, the loss rates of DMBT1 mRNA expression in patients with lymph node metastasis and clinical stage Ⅲ were higher than that in patients without metastasis and clinicalⅠⅡstage (χ2=4.885, P=0.026; χ2=4.600, P=0.032). ConclusionLow expression of DMBT1 mRNA is closely associated with the metastasis and clinical stage of breast cancer.

Key words: Ｂｒｅａｓｔｎｅｏｐｌａｓｍｓ, ＤＭＢＴ１ｇｅｎｅ