国际肿瘤学杂志

国际肿瘤学杂志 ›› 2014, Vol. 41 ›› Issue (10): 779-781.doi: １０．３７６０／ｃｍａ．ｊ．ｉｓｓｎ．１６７３４２２Ｘ．２０１４．１０．０１８

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人类白细胞抗原HLA-DQA1基因多态性在 尘肺肺癌患者中的表达

赵娜, 杨锡贵, 曹殿凤, 高萍, 张宝玲   

  1. ２５００１７济南，山东省医学科学院 山东省肿瘤医院内
    科；济南大学 山东省医学科学院医学与生命科学学院（赵娜、杨锡
    贵）；淄博市职业病防治院（曹殿凤、高萍、张宝玲）
  • 出版日期:2014-10-29 发布日期:2014-10-11
  • 通讯作者: 赵娜，Ｅｍａｉｌ：１０９５８０５６６＠ｑｑ．ｃｏｍ E-mail:１０９５８０５６６＠ｑｑ．ｃｏｍ

Expression of HLADQA1 gene polymorphism in the lung cancer patients with pneumoconiosis

ZHAO  Na, YANG  Xi-Gui, CAO  Dian-Feng, GAO  Ping, ZHANG  Bao-Ling   

  1. Department of Internal MedicineOncology, Shandong Cancer Hospital, Shandong Academy of Medical Sciences; School of Medicine and Life Sciences, University of Ji′nanShandong Academy of Medical Sciences, Ji′nan 250017, China
  • Online:2014-10-29 Published:2014-10-11
  • Contact: ZHAO Na E-mail:１０９５８０５６６＠ｑｑ．ｃｏｍ

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摘要： 目的　检测人类白细胞抗原（ＨＬＡ）ＤＱＡ１位点基因多态性在尘肺肺癌患者与非职业性肺 癌患者的表达差异。方法　用多重聚合酶链反应连接酶检测反应分型法（ＰＣＲＬＤＲ），分别检测 ２１例 尘肺并发肺癌患者及４０例非职业性肺癌患者的 ＨＬＡＤＱＡ１共６个位点的等位基因，并分析其和尘肺肺 癌发病的相关性。结果　尘肺肺癌组患者 ＨＬＡＤＱＡ１０３０１基因频率 １９％，非职业性肺癌组患者 ＨＬＡ ＤＱＡ１０３０１基因频率２．５％，差异有统计学意义（χ ２＝１０．０２２，Ｐ＝０．００２），其余等位基因两组差异无统计 学意义。结论　ＨＬＡＤＱＡ１的基因多态性可能与尘肺肺癌易感性相关。ＤＱＡ１０３０１是尘肺并发肺癌的 重要的调控基因之一，可能为尘肺肺癌易感的危险因素。

关键词: 尘肺, 肺肿瘤, 基因多态性

Abstract: ObjectiveTo detect the difference of the expression  of HLADQA1 genes polymorphism in the lung cancer patients with pneumoconiosis and the nonoccupational lung cancer patients. MethodsMultiple polymerase chain reactionligase detection reaction classification method (PCRLDR) was used to detect 6 alleles in HLADQA1 and the expression of HLADQA1 gene between the 21 cases of pneumoconiosis complicated with lung cancer and 40 cases of nonoccupational lung cancer respectively. The correlation between them with pneumoconiosis complicated with lung cancer was analysed. ResultsHLADQA1 0301 gene frequencies were 19% in pneumoconiosis complicated with lung cancer group and 2.5% in nonoccupational lung cancer group respectively,  with a statistically significant difference (χ2=10.022, P=0.002). There was no statistically significant difference in the other allele between the two groups. ConclusionHLADQA1 gene polymorphism may be related to the susceptibility of the pneumoconiosis complicated with lung cancer. DQA1 0301 is one of the important genes of pneumoconiosis complicated with lung cancer, which maybe the risk factor for the susceptibility of the pneumoconiosis complicated with lung cancer.

Key words: Pneumoconiosis, Lung neoplasms, Gene polymorphism