国际肿瘤学杂志 ›› 2013, Vol. 40 ›› Issue (4): 267-270.

• 综述 • 上一篇    下一篇

多重基因表达遗传分析系统及其在肿瘤研究中的应用

张寒, 郑胡镛   

  1. 100045 首都医科大学附属北京儿童医院 血液病中心
  • 出版日期:2013-04-08 发布日期:2013-03-27
  • 通讯作者: 郑胡镛,E-mail:zhenghuyong@vip.sina.com E-mail:zhenghuyong@vip.sina.com
  • 基金资助:

    国家自然科学基金(81070454)

Multiplex gene expression genetic analysis system and its pplication in cancer research

ZHANG  Han, ZHENG  Hu-Yong   

  1. Hematology Oncology Center, Beijing Children’s Hospital, Capital Medical University, Beijing 100045, China
  • Online:2013-04-08 Published:2013-03-27
  • Contact: ZHENG Hu-yong E-mail:zhenghuyong@vip.sina.com

摘要: 多重基因表达遗传分析系统是一种全新的基因表达谱定量分析平台。它采用荧光毛细电泳分离技术,巧妙地将通用引物与特异引物相结合,实现对多重PCR产物进行定量检测和分析。该技术简便快捷、产率高效,在肿瘤分型及标记物研究中具有广阔的应用前景。

关键词: 基因表达, 肿瘤, 分型, 生物学标记, 多重PCR

Abstract: The multiplex gene expression genetic analysis system is a novel analytical platform, characterized by its primer design with gene-specific and universal sequences. The fluorescence capillary electrophoretic separation is used to compare and analyze the expression levels of multiplex PCR products. This technology is simple, rapid, productive and cost-effective, and holds great promise for cancer classification and biomarkers discovery.

Key words: Gene expression, Neoplasms, Classification, Biological markers, Multiplex polymerase chain reaction