国际肿瘤学杂志

国际肿瘤学杂志 ›› 2012, Vol. 39 ›› Issue (8): 713-717.

• 论著 • 上一篇    下一篇

Survivin和环氧合酶2在壶腹癌中的表达及临床意义

薛栋,左凯,李新军,王建强,张成德,成丕光,张同军,巩本刚   

  1. 256610 山东省滨州市人民医院肝胆外科（薛栋、王建强、张成德、成丕光、张同军、巩本刚），感染二科（左凯），病理科（李新军）
  • 出版日期:2012-09-22 发布日期:2012-09-24

Expression and clinical significance of Survivin and COX-2 in human ampullary carcinoma

XUE  Dong, ZUO  Kai, LI  Xin-Jun, WANG  Jian-Qiang, ZHANG  Cheng-De, CHENG  Pi-Guang, ZHANG  Tong-Jun, GONG  Ben-Gang   

  1. Department of  Hepatobiliary Surgery, People′s Hospital of Binzhou City, Binzhou 256610, China
  • Online:2012-09-22 Published:2012-09-24

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摘要： 目的 探讨Survivin和环氧合酶-2（COX-2）蛋白在壶腹癌组织中的表达及其临床意义.方法 用免疫组化EnVision法对40例壶腹癌组织进行Survivin和COX-2蛋白检测，并对8例正常壶腹组织进行对照研究.结果 壶腹癌组织中33例Survivin蛋白呈阳性表达，阳性率为82.5％,对照组中无阳性表达，两组阳性率比较，差异有统计学意义(P<0.01)；Survivin表达与十二指肠浸润、胰腺浸润、淋巴结转移均有明显相关性(均P<0.05)。在壶腹癌组中27例COX-2蛋白阳性表达，阳性率为67.5％，而对照组8例无阳性表达，两组阳性率比较差异有统计学意义(P<0.01)；COX-2表达与十二指肠浸润、胰腺浸润、淋巴结转移均有明显相关性(均P<0.05)。经Spearman等级相关性分析表明，Survivin与COX-2蛋白表达呈正相关(r=0.383,P=0.015)。结论 Survivin基因和COX-2基因的异常表达可能对壶腹癌的发生和发展起重要作用，可能为壶腹癌的早期诊断和基因治疗提供新的靶点。

关键词: 肝胰管壶腹, 肿瘤, Survivin, 环氧化酶-2, 免疫组织化学

Abstract: Objective To study the expression of Survivin and COX-2 in ampullary carcinoma and their clinical significance.Methods The expression of Survivin and COX-2 proteins were tested by EnVision immunohistochemistry in 40 cases of ampullary carcinomas，and 8 cases of normal ampulla of vater as the controls．Results The positive rate of Survivin in ampullary carcinomas was significantly higher than that of the controls(82.5％vs 0，P<0.01). The expression of Survivin in ampullary carcinoma was correlated with duodenal invasion,pancreatic invasion  and lymph node metastasis(P<0.05).    Significant difference was also observed in the expression rate of COX2 between the patients with ampullary carcinoma and the normal controls (67.5％vs 0，P<0.01).The expression of COX-2 in ampullary carcinoma was correlated with duodenal invasion，pancreatic invasion and lymph node metastasis (P<0.05). Significantly positive correlation was found between the expression of Survivin and COX-2 by using spearman correlation analysis(r=0.383, P=0.015)．Conclusion The specific upregulation of COX-2 gene and Survivin gene may play an important role in the genesis and development of ampullary carcinoma.  COX-2 and Survivin may be  used as early diagnosis markers and  potential therapeutic targets in ampullary carcinoma.

Key words: Ampulla of vater, Neoplasms, Survivin, Cyclooxygenase-2, Immunohischemistry