国际肿瘤学杂志

国际肿瘤学杂志 ›› 2018, Vol. 45 ›› Issue (4): 214-219.doi: 10.3760/cma.j.issn.1673-422X.2018.04.006

• 论著 • 上一篇    下一篇

免疫球蛋白样转录子3在结直肠癌中的表达及临床意义

路春晓,刘杰,刘传勇   

  1. 261053 潍坊医学院肿瘤学教研室（路春晓）；山东大学附属济南市中心医院肿瘤科（刘杰、刘传勇）
  • 出版日期:2018-04-08 发布日期:2018-05-16
  • 通讯作者: 刘传勇，Email: cyl0936@sina.com E-mail:cyl0936@sina.com

Expression and clinical significance of immunoglobulin-like transcript 3 in colorectal cancer

Lu Chunxiao, Liu Jie, Liu Chuanyong   

  1. Department of Oncology, Weifang Medical University, Weifang 261053, China
  • Online:2018-04-08 Published:2018-05-16
  • Contact: Liu Chuanyong, Email: cyl0936@sina.com E-mail:cyl0936@sina.com

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摘要： 目的 探讨免疫球蛋白样转录子3（ILT3）在结直肠癌组织及癌细胞株中的表达与临床病理特征及患者预后的关系，揭示其在结直肠癌中异常表达的临床意义。方法 采用实时荧光定量PCR和Western blotting方法检测结直肠癌细胞株SW480、SW620、HT-29和人正常肠上皮细胞株FHC中ILT3的mRNA和蛋白表达，并分析其差异。采用免疫组织化学法检测85例结直肠癌患者病理组织及30例癌旁正常结直肠黏膜组织中ILT3蛋白的表达，分析其与临床病理特征及患者预后的关系。结果 结直肠癌细胞株SW480、SW620、HT-29及正常肠上皮细胞株FHC中ILT3 mRNA的相对表达量分别为10.813±3.289、4.391±0.622、1.481±0.055、1.000±0.000，差异有统计学意义（F=21.846，P<0.001）。与正常肠上皮细胞株FHC相比，ILT3 mRNA在癌细胞株SW480、SW620中的相对表达量明显增强，差异有统计学意义（P<0.001，P=0.038），在癌细胞株HT-29中相对表达量较强，但差异无统计学意义（P=0.734）。结直肠癌细胞株SW480、SW620、HT-29及正常肠上皮细胞株FHC中ILT3蛋白的相对表达量分别为1.838±0.651、1.763±0.222、1.449±0.200、0.628±0.263，差异有统计学意义（F=6.320，P=0.017）。与正常肠上皮细胞株FHC相比，ILT3蛋白在癌细胞株SW480、SW620、HT29中的相对表达量明显增强，差异有统计学意义（P=0.005，P=0.007，P=0.030）。ILT3主要在细胞膜和（或）细胞质中表达，85例结直肠癌病理组织中，64.7%（55/85）ILT3高表达，显著高于癌旁正常组织的16.7%（5/30），差异有统计学意义（χ2=20.508，P＜0.001）。ILT3高表达与肿瘤淋巴结转移（χ2=4.684，P=0.030）及TNM分期（χ2=4.684，P=0.030）相关，而与年龄（χ2=0.927，P=0.336）、性别（χ2=0.078，P=0.780）、肿瘤发生部位（χ2=0.249，P=0.618）、肿瘤大小（χ2=1.813，P=0.178）及肿瘤分化程度（χ2=0.807，P=0.369）无关。ILT3高表达组患者的中位总生存期短于ILT3低表达组患者（45.0个月∶74.0个月），差异有统计学意义（χ2＝17.907，P＜0.001）。结论 结直肠癌细胞株中ILT3的表达高于人正常肠上皮细胞株，结直肠癌组织中ILT3的表达高于癌旁正常组织，且其高表达与肿瘤淋巴结转移及TNM分期相关。ILT3高表达预示患者临床预后差，提示其可能作为结直肠癌诊治及判断预后的新靶点。

关键词: 结直肠肿瘤, 预后, 免疫球蛋白样转录因子3

Abstract: Objective To investigate the expression of immunoglobulin-like transcript 3 (ILT3) in colorectal cancer tissues and colorectal cancer cell lines, and the correlations with the clinicopathological factors and prognosis, and to reveal the clinical significance of ILT3 abnormal expression in colorectal cancer. Methods The mRNA and protein expressions of ILT3 in colorectal cancer cell lines SW480, SW620, HT-29 and human normal intestinal epithelial cell line FHC were detected by real-time fluorescence quantitative PCR and Western blotting, and the differences were analyzed. Immunohistochemistry was used to detect the expressions of ILT3 in 85 colorectal cancer tissues and 30 adjacent normal tissues. The correlations between the expression of ILT3 and clinicopathological factors and prognosis were analyzed. Results The relative expressions of ILT3 mRNA in cancer cell lines SW480, SW620, HT-29 and the normal intestinal epithelial cells line FHC were 10.813±3.289, 4.391±0.622, 1.481±0.055 and 1.000±0.000 respectively, with a significant difference (F=21.846, P<0.001). Compared with the normal intestinal epithelial cell line FHC, the relative expressions of ILT3 mRNA in cancer cell lines SW480 and SW620 were significantly higher, with a statistically significant difference (P<0.001, P=0.038), and the relative expression of ILT3 mRNA in cancer cell HT-29 was higher, but the difference has no statistical significance (P=0.734). The relative expressions of ILT3 protein in cancer cell lines SW480, SW620, HT-29 and normal intestinal epithelial cells line FHC were 1.838±0.651, 1.763±0.222, 1.449±0.200 and 0.628±0.263 respectively, with a statistically significant difference (F=6.320, P=0.017). Compared with the normal intestinal epithelial cell line FHC, the relative expressions of ILT3 protein in cancer cell lines SW480, SW620, HT-29 were significantly higher, with a statistically significant difference(P=0.005, P=0.007, P=0.030). ILT3 was mainly expressed in the membrane and (or) cytoplasm. In 85 cases of colorectal cancer tissues, 55 cases (64.7%) have ILT3 high expressions, which were significantly higher than those in the adjacent normal tissues (16.7%, 5/30), with a significant difference (χ2=20.508, P<0.001). The high expression of ILT3 was related to lymph node metastasis (χ2=4.684, P=0.030) and TNM staging (χ2=4.684, P=0.030), but it was not related to age (χ2=0.927, P=0.336), gender (χ2=0.078, P=0.780), tumor location (χ2=0.249, P=0.618), tumor size (χ2=1.813, P=0.178) and tumor differentiation (χ2=0.807, P=0.369). The median overall survival of patients with high ILT3 expression was significantly shorter than that of patients with low ILT3 expression (45.0 months vs. 74.0 months), with a significant difference (χ2＝17.907, P<0.001). Conclusion The expressions of ILT3 in colorectal cancer cells are higher than those in the normal intestinal epithelial cell line, the expressions of ILT3 in colorectal tissues are higher than those in the adjacent normal tissues, which are correlated with lymph node metastasis and TNM staging. Patients with high expressions of ILT3 indicate poor clinical prognosis. The expression of ILT3 may serves as a new target for diagnosis, treatment and prognosis of colorectal cancer.

Key words: Colorectal neoplasms, Prognosis, Immunoglobulin like transcription factor 3