基于适配子的分子探针在肿瘤分子影像学中的应用

doi:10.3760/cma.j.issn.1673-422X.2016.11.009

国际肿瘤学杂志 ›› 2016, Vol. 43 ›› Issue (11): 838-840.doi: 10.3760/cma.j.issn.1673-422X.2016.11.009

基于适配子的分子探针在肿瘤分子影像学中的应用

石玉生, 彭勇华, 盛树悦, 张兴梅

510515 广州，南方医科大学南方医院放疗科（石玉生）；南方医科大学基础医学院神经生物学教研室（彭勇华、张兴梅），第一临床医学院（盛树悦）

收稿日期:2016-03-22 出版日期:2016-11-08 发布日期:2016-11-02
通讯作者: 张兴梅，Email：zxmray@hotmail.com E-mail:zxmray@hotmail.com
基金资助:
国家自然科学基金（81272509、30973481）；南方医科大学南方医院院长基金（2015Z007）；广东省自然科学基金（2014A030313351）

Aptamers as molecular probes in tumor molecular imaging

Shi Yusheng, Peng Yonghua, Sheng Shuyue, Zhang Xingmei

Department of Radiotherapy, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China

Received:2016-03-22 Online:2016-11-08 Published:2016-11-02
Contact: Zhang Xingmei E-mail:zxmray@hotmail.com
Supported by:
National Natural Science Foundation of China (81272509, 30973481); President Foundation of Nanfang Hospital of Southern Medical University (2015Z007); Natural Science Foundation of Guangdong Province of China (2014A030313351)

摘要/Abstract

摘要： 理想的分子影像探针应具有一定的特异性、敏感性、安全性。适配子是可与靶分子高特异性和高亲和力结合的单链DNA或RNA分子，具有靶分子广泛、分子小、易生产和修饰、免疫原性低、渗透性强等特点，是一种新型的分子影像探针。目前基于适配子的肿瘤分子影像学研究越来越多，为肿瘤的诊断和治疗提供了新的技术和工具。

关键词: 分子探针, 适体, 核苷, 肿瘤

Abstract: The ideal molecular imaging probe should have characteristics of specificity, sensitivity and safety. Aptamers are singstrand DNA or RNA oligonucleotides which can bind their targets with high specificity and high affinity. Aptamers are new types of molecular imaging probe because they have many advantages, such as their large sum of possible targets, small molecule weights, easily production and modification, low immunogenicity, high tissue penetration and so on. Now more and more aptamers research on tumor molecular imaging provides a new technology and tool for tumor diagnosis and therapy.

Key words: Molecular probes, Aptamers, nucleotide, Neoplasms

石玉生, 彭勇华, 盛树悦, 张兴梅. 基于适配子的分子探针在肿瘤分子影像学中的应用[J]. 国际肿瘤学杂志, 2016, 43(11): 838-840.

Shi Yusheng, Peng Yonghua, Sheng Shuyue, Zhang Xingmei. Aptamers as molecular probes in tumor molecular imaging[J]. Journal of International Oncology, 2016, 43(11): 838-840.

参考文献

［1］申宝忠. 无限潜能魅力彰显——分子影像学研究的回顾和展望［J］. 中华放射学杂志, 2014, 48(5): 353-357. ［2］ Hong H, Goel S, Zhang Y, et al. Molecular imaging with nucleic acid aptamers［J］. Curr Med Chem, 2011, 18(27): 4195-4205. ［3］ Rozenblum GT, Lopez VG, Vitullo AD, et al. Aptamers: current challenges and future prospects［J］. Expert Opin Drug Discov, 2015, 18: 1-9. DOI: 10.1517/17460441.2016.1126244. ［4］ Hernandez LI, Machado I, Schafer T, et al. Aptamers overview: selection, features and applications［J］. Curr Top Med Chem, 2015, 15(12): 1066-1081. ［5］ Wang AZ, Farokhzad OC. Current progress of aptamerbased molecular imaging［J］. J Nucl Med, 2014, 55(3): 353-356. DOI: 10.2967/jnumed.113.126144. ［6］ Hicke BJ, Stephens AW, Gould T, et al. Tumor targeting by an aptamer［J］. J Nucl Med, 2006, 47(4): 668-678. ［7］ Wu X, Liang H, Tan Y, et al. CellSELEX apt6amer for highly specific radionuclide molecular imaging of glioblastoma in vivo［J］. PLoS One, 2014, 9(6): e90752. DOI: 10.1371/journal.pone.0090752. ［8］ Jacobson O, Yan X, Niu G, et al. PET imaging of tenascinC with a radiolabeled singlestranded DNA aptamer［J］. J Nucl Med, 2015, 56(4): 616-621. DOI: 10.2967/jnumed.114.149484. ［9］ Li J, Zheng H, Bates PJ, et al. Aptamer imaging with Cu64 labeled AS1411: preliminary assessment in lung cancer［J］. Nucl Med Biol, 2014, 41(2): 179-185. DOI: 10.1016/j.nucmedbio.2013.10.008. ［10］ Chen T, Shukoor MI, Wang R, et al. Smart multifunctional nanostructure for targeted cancer chemotherapy and magnetic resonance imaging［J］. ACS Nano, 2011, 5(10): 7866-7873. DOI: 10.1021/nn202073m. ［11］ Wang AZ, Bagalkot V, Vasilliou CC, et al. Superparamagnetic iron oxide nanoparticleaptamer bioconjugates for combined prostate cancer imaging and therapy［J］. Chem Med Chem, 2008, 3(9): 13111315. DOI: 10.1002/cmdc.200800091. ［12］ Wang CH, Kang ST, Lee YH, et al. Aptamerconjugated and drugloaded acoustic droplets for ultrasound theranosis［J］. Biomaterials, 2012, 33(6): 1939-1947. DOI: 10.1016/j.biomaterials.2011.11.036. ［13］ Tong R, Coyle VJ, Tang L, et al. Polylactide nanoparticles containing stably incorporated cyanine dyes for in vitro and in vivo imaging applications［J］. Microsc Res Tech, 2010, 73(9): 901-909. DOI: 10.1002/jemt.20824. ［14］ Mi J, Zhang X, Giangrande PH, et al. Targeted inhibition of alphavbeta3 integrin with an RNA aptamer impairs endothelial cell growth and survival［J］. Biochem Biophys Res Commun, 2005, 338(2): 956-963. ［15］ Alibolandi M, Abnous K, Ramezani M, et al. Synthesis of AS1411aptamerconjugated CdTe quantum dots with high fluorescence strength for probe labeling tumor cells［J］. J Fluoresc, 2014, 24(5): 1519-1529. DOI : 10.1007/s10895-014-1437-5. ［16］ Kim Y, Wu Q, Hamerlik P, et al. Aptamer identification of brain tumorinitiating cells［J］. Cancer Res, 2013, 73(15): 4923-4936. DOI: 10.1158/0008-5472.CAN-12-4556. ［17］ Martínez O, Bellard E, Golzio M, et al. Direct validation of aptamers as powerful tools to image solid tumor［J］. Nucleic Acid Ther, 2014, 24(3): 217-225. DOI: 10.1089/nat.2013.0444. ［18］ Shi H, He X, Wang K, et al. Activatable aptamer probe for contrastenhanced in vivo cancer imaging based on cell membrane proteintriggered conformation alteration［J］. Proc Natl Acad Sci USA, 2011, 108(10): 3900-3905. DOI: 10.1073/pnas.1016197108. ［19］ Li L, Tong R, Chu H, et al. Aptamer photoregulation in vivo［J］. Proc Natl Acad Sci USA, 2014, 111(48): 17099-17103. DOI: 10.1073/pnas.1420105111.

[1]	刘娜, 寇介丽, 杨枫, 刘桃桃, 李丹萍, 韩君蕊, 杨立洲. 血清miR-106b-5p、miR-760联合低剂量螺旋CT诊断早期肺癌的临床价值[J]. 国际肿瘤学杂志, 2024, 51(6): 321-325.
[2]	杨蜜, 别俊, 张加勇, 邓佳秀, 唐组阁, 卢俊. 局部晚期可切除食管癌新辅助治疗疗效及预后分析[J]. 国际肿瘤学杂志, 2024, 51(6): 332-337.
[3]	袁健, 黄燕华. Hp-IgG抗体联合血清DKK1、sB7-H3对早期胃癌的诊断价值[J]. 国际肿瘤学杂志, 2024, 51(6): 338-343.
[4]	陈红健, 张素青. 血清miR-24-3p、H2AFX与肝癌患者临床病理特征及术后复发的关系研究[J]. 国际肿瘤学杂志, 2024, 51(6): 344-349.
[5]	郭泽浩, 张俊旺. PFDN及其亚基在肿瘤发生发展中的作用[J]. 国际肿瘤学杂志, 2024, 51(6): 350-353.
[6]	张百红, 岳红云. 新作用机制的抗肿瘤药物进展[J]. 国际肿瘤学杂志, 2024, 51(6): 354-358.
[7]	许凤琳, 吴刚. EBV在鼻咽癌肿瘤免疫微环境和免疫治疗中的研究进展[J]. 国际肿瘤学杂志, 2024, 51(6): 359-363.
[8]	王盈, 刘楠, 郭兵. 抗体药物偶联物在转移性乳腺癌治疗中的研究进展[J]. 国际肿瘤学杂志, 2024, 51(6): 364-369.
[9]	张蕊, 褚衍六. 基于FIT与肠道菌群的结直肠癌风险评估模型的研究进展[J]. 国际肿瘤学杂志, 2024, 51(6): 370-375.
[10]	高凡, 王萍, 杜超, 褚衍六. 肠道菌群与结直肠癌非手术治疗的相关研究进展[J]. 国际肿瘤学杂志, 2024, 51(6): 376-381.
[11]	王丽, 刘志华, 杨伟洪, 蒋凤莲, 李全泳, 宋浩杰, 鞠文东. ROS1突变肺腺鳞癌合并脑梗死为主要表现的Trousseau综合征1例[J]. 国际肿瘤学杂志, 2024, 51(6): 382-384.
[12]	刘静, 刘芹, 黄梅. 基于SMOTE算法的食管癌放化疗患者肺部感染的预后模型构建[J]. 国际肿瘤学杂志, 2024, 51(5): 267-273.
[13]	杨琳, 路宁, 温华, 张明鑫, 朱琳. 炎症负荷指数与胃癌临床关系研究[J]. 国际肿瘤学杂志, 2024, 51(5): 274-279.
[14]	王俊毅, 洪楷彬, 纪荣佳, 陈大朝. 癌结节对结直肠癌根治性切除术后肝转移的影响[J]. 国际肿瘤学杂志, 2024, 51(5): 280-285.
[15]	张宁宁, 杨哲, 檀丽梅, 李振宁, 王迪, 魏永志. 宫颈细胞DNA倍体分析联合B7-H4和PKCδ对宫颈癌的诊断价值[J]. 国际肿瘤学杂志, 2024, 51(5): 286-291.

基于适配子的分子探针在肿瘤分子影像学中的应用

Aptamers as molecular probes in tumor molecular imaging

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价