国际肿瘤学杂志

国际肿瘤学杂志 ›› 2015, Vol. 42 ›› Issue (6): 414-418.doi: 10.3760/cma.j.issn.1673-422X.2015.06.004

• 论著 • 上一篇    下一篇

血清DKK1和P53自身抗体联合检测对食管鳞状细胞癌的诊断价值

彭裕辉,陈建良,翁雪芬,方裕森,许镒洧   

  1. 515041汕头大学医学院附属肿瘤医院检验科
  • 收稿日期:2014-11-27 出版日期:2015-06-08 发布日期:2015-05-31
  • 通讯作者: 许镒洧，Email：yiwei512@126.com E-mail:yiwei512@126.com
  • 基金资助:

    广东省科技计划(2013B021800250)；汕头市医疗科技计划［(2013)88］

Combined detection of serum DKK1 and P53 autoantibodies for the diagnostic value of esophageal squamous cell carcinoma

Peng Yuhui, Chen Jianliang, Weng Xuefen, Fang Yusen, Xu Yiwei   

  1. Department of Clinical Laboratory, Cancer Hospital of Shantou University Medical College, Shantou 515041, China
  • Received:2014-11-27 Online:2015-06-08 Published:2015-05-31
  • Contact: Xu Yiwei E-mail:yiwei512@126.com

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摘要： 目的 探讨血清DKK1（Dickkopf-1）和P53自身抗体联合检测在食管鳞状细胞癌（ESCC）中的诊断意义。方法 应用酶联免疫吸附试验检测126例ESCC患者和60例正常对照血清DKK1和P53自身抗体的表达水平，采用受试者工作特征曲线（ROC）评价诊断效能。结果 血清DKK1水平和P53自身抗体在ESCC患者中的表达均明显高于正常对照［(673.09±343.82)pg/ml∶(362.05±148.07)pg/ml，Z=6.158，P＜0.000 1；(0.398±0.546)∶(0.069±0.050)，Z=3.832，P＜0.000 1］。ROC曲线显示，当血清DKK1为最佳诊断临界值588.77 pg/ml时，其在诊断ESCC的曲线下面积（AUC）为0.780（95%CI 为0.715～0.844，敏感性为61.9%，特异性为95.0%）。P53自身抗体诊断ESCC的AUC为0.674（95%CI为0.598～0.750，敏感性为45.3%，特异性为95.0%）。DKK1和P53自身抗体联合检测诊断食管癌的AUC为0.843（95%CI为0.788～0.897，敏感性为73.8%，特异性为95.0%）。在早期ESCC，DKK1和P53自身抗体联合检测具有更好的诊断效能，AUC为0.903（95%CI为0.845～0.961，敏感性为81.0%，特异性为95.0%）。结论 血清DKK1和P53自身抗体可作为ESCC的血清诊断标志物，联合检测有助于ESCC的早期诊断。

关键词: 食管肿瘤, 诊断, Dickkopf-1, P53自身抗体

Abstract: Objective To evaluate the diagnostic value of the combination of serum DKK1(Dickkopf1) and P53 autoantibodies in patients with esophageal squamous cell carcinoma (ESCC). Methods Serum levels of DKK1 and P53 autoantibodies were measured by enzymelinked immunosorbent assay (ELISA) for the 126 patients with ESCC and 60 normal controls. Receiver operating characteristics (ROC) was used to calculate the diagnostic efficiency. Results The serum levels of DKK1 and P53 autoantibodies were significantly higher in ESCC than those in normal controls ［(673.09±343.82)pg/ml vs (362.05±148.07)pg/ml, Z=6.158, P＜0.000 1; (0.398±0.546) vs (0.069±0.050), Z=3.832, P＜0.000 1］. ROC curves showed the optimum diagnostic cutoff for serum DKK1 was 588.77 pg/ml, with an area under curve (AUC) of 0.780 (95%CI: 0.715～0.844, 61.9% sensitivity, 95.0% specificity). Measurement of P53 autoantibodies demonstrated an AUC of 0.674 (95%CI: 0.598～0.750, 45.3% sensitivity, 95.0% specificity). The combination of DKK1 and P53 autoantibodies yielded an AUC of 0.843 (95%CI: 0.788～0.897, 73.8% sensitivity, 95.0% specificity). In early stage ESCC, combined detection of DKK1 and P53 autoantibodies improved the diagnostic power, with an AUC of 0.903 (95%CI: 0.845～0.961, 81.0% sensitivity, 95.0% specificity). Conclusion Serum DKK1 and P53 autoantibodies can be used as potential diagnostic biomarkers for the ESCC. Combined detection of them might aid the early diagnosis of ESCC.

Key words: Esophageal neoplasms, Diagnosis, Dickkopf-1, P53 autoantibody