国际肿瘤学杂志

国际肿瘤学杂志 ›› 2015, Vol. 42 ›› Issue (12): 903-906.doi: 10.3760/cma.j.issn.1673-422X.2015.12.006

• 论著 • 上一篇    下一篇

Pokemon在原发性肝癌中的表达及诊断价值

张全乐,邢喜芝,李风艳,秦燕鸿,张立英   

  1. 061000 河北省沧州中西医结合医院消化内科（张全乐、邢喜芝、秦燕鸿、张立英），放射科（李风艳）
  • 出版日期:2015-12-08 发布日期:2015-11-10
  • 通讯作者: 张全乐 E-mail:zhangquanle2008@hotmail.com
  • 基金资助:


    DOI：10.3760/cma.j.issn.1673422X.2015.12.006基金项目：河北省卫生计生委科技支持项目（ZD20140129）

Expression and clinical value of Pokemon in hepatocellular carcinoma

Zhang Quanle, Xing Xizhi, Li Fengyan, Qin Yanhong, Zhang Liying   

  1. Department of Gastroenterology, Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine of Hebei Province, Cangzhou 061000, China
  • Online:2015-12-08 Published:2015-11-10
  • Contact: Zhang Quanle E-mail:zhangquanle2008@hotmail.com

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摘要： 目的研究Pokemon在原发性肝细胞癌（HCC）患者血清中的表达，比较Pokemon、甲胎蛋白（AFP）、AFPL3、γ羧基凝血酶原（DCP）对HCC的诊断价值。方法将研究对象分为3组，HCC组30例、良性肝病组34例、正常对照组30例，酶联免疫吸附试验（ELISA）检测3组血清中Pokemon、AFP、AFPL3、DCP的表达水平并分析差异；通过受试者工作特征（ROC）曲线获得Pokemon、AFP、AFPL3、DCP的最佳诊断界值，并对4种标志物的敏感性、特异性进行分析。结果HCC组血清中Pokemon表达量为（22.63±6.82）ng/ml，高于良性肝病组的（3.54±1.26）ng/ml及正常对照组的（1.95±0.57）ng/ml（t=8.594，P＜0.001；t=10.021，P＜0.001）。Pokemon的表达量与HCC患者的肿瘤直径（t=2.678，P=0.021）、TNM分期（t=2.578，P=0.034）有关。HCC组AFP、AFPL3、DCP的表达量分别为（774.56±96.52）ng/ml、（26.37±2.54）ng/ml、（93.49±30.45）mAU/ml，良性肝病组分别为（22.21±3.57）ng/ml、(7.05±2.71)ng/ml、(34.68±10.13)mAU/ml，正常对照组分别为（14.65±4.45）ng/ml、（3.84±1.09）ng/ml、（25.87±9.01）mAU/ml，三组间差异均有统计学意义（F=58.46，P=0.000；F=12.47，P=0.000；F=23.41，P=0.000）。Pokemon、AFP、AFPL3、DCP诊断HCC的敏感性和特异性分别为84.6%和87.5%、52.5%和78.9%、95.0%和80.0%、77.1%和87.5%。结论Pokemon在HCC患者血清中的表达明显升高，其诊断HCC的敏感性及特异性高于AFP，提示Pokemon对HCC的诊断有一定价值。

关键词: 肝肿瘤, 酶联免疫吸附测定, Pokemon蛋白

Abstract: ObjectiveTo detect the serum expression level of Pokemon and explore its clinical value combines with AFP, AFPL3 or DCP in hepatocellular carcinoma (HCC). MethodsIn this study, the serum levels of Pokemon, AFP, AFPL3 and DCP in 30 patients with HCC, 34 patients with benign liver diseases and 30 healthy controls were examined by enzymelinked immunosorbent assay. Then, the clinical significance of Pokemon expression level was analyzed. In the end, the diagnostic evaluation of four serum biomarker alone detection was used to analyze the sensitivity, specificity, the area under the curve and Youden index in the diagnosis of HCC. ResultsThe serum expression level of Pokemon in patients with HCC was (22.63±6.82)ng/ml, which was significantly higher than that in the group with benign liver diseases and healthy controls ［(3.54±1.26)ng/ml, t=8.594, P＜0.001; (1.95±0.57)ng/ml, t=10.021, P＜0.001］. The expression level of Pokemon in HCC patients was correlated to tumor size (t=2.678, P=0.021) and TNM stag (t=2.578, P=0.034). The serum expression levels of AFP, AFPL3 and DCP in patients with HCC were (774.56±96.52)ng/ml, (26.37±2.54)ng/ml and (93.49±30.45)mAU/ml, which were significantly higher than those in the group with benign liver diseases ［(22.21±3.57)ng/ml, (7.05±2.71)ng/ml, (34.68±10.13)mAU/ml］ and healthy controls ［(14.65±4.45)ng/ml, (3.84±1.09)ng/ml, (25.87±9.01)mAU/ml, F=58.46, P=0.000; F=12.47, P=0.000; F=23.41, P=0.000］. The sensitivity and specificity of Pokemon, AFP, AFPL3, DCP in the diagnosis of HCC were 84.6% and 87.5%, 52.5% and 78.9%, 95.0% and 80.0%, 77.1% and 87.5%, respectively. ConclusionThe enhanced Pokemon serum level， with its sensitivity and specificity higher than AFP, points out Pokemon may be a potentially useful biomarker to diagnose HCC.

Key words: Liver neoplasms, Enzymelinked immunosorbent assay, Pokemon protein