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    08 March 2018, Volume 45 Issue 3 Previous Issue    Next Issue
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    Effects of miR-1291 on the cell cycle and proliferation of renal cell carcinoma by regulating the expression of Zinc finger protein 8 gene
    Ye Zhihua, Huang Geng, Fu Jinlun, Gui Dingwen
    2018, 45 (3):  129-133.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.001
    Abstract ( 400 )   PDF (944KB) ( 822 )   Save
    Objective To investigate the effects of microRNA-1291 (miR-1291) on the expression of Zinc finger protein 8 (PHF8) gene in renal cell carcinoma and its effect on cell cycle and proliferation of renal cell carcinoma. MethodsRealtime fluorescence quantitative polymerase chain reaction (qRT-PCR) was used to detect the expression of miR1291 in renal cell carcinoma cell lines OS-RC-2, ACHN, A498, 786-O and human proximal tubular epithelial cells HK-2. miR-1291 (miR-1291 group) and miR-NC (miR-NC group) were transfected into the renal cell lines with the lowest expression of miR1291. qRTPCR was used to detect the expression of miR-1291 and PHF8 mRNA in the transfected cells. The expression levels of PHF8, Cyclindependent kinase 6 (CDK6) and Cyclin D1 were detected by Western blotting. The effect of miR-1291 on the transcriptional activity of PHF8 was detected by double luciferase reporter gene system. Flow cytometry was used to detect cell cycle distribution. Methyl thiazolyl tetrazolium (MTT) assay and colony formation assay were used to detect cell viability and proliferation. ResultsThe expressions of miR1291 in renal carcinoma cell lines OS-RC-2, ACHN, A498, 786-O and human proximal renal tubular epithelial cell HK2 were 0.64±0.17, 0.60±0.15, 0.29±0.08, 0.63±0.08 and 1.01±0.17 respectively, with a significant difference (F=13.790, P<0.001). Compared with renal carcinoma cell lines OS-RC-2, ACHN and 786-O, the expression level of miR-1291 in A498 cell line was the lowest (P=0.002, P=0.006, P=0.003). The expression levels of miR1291 in A498 cell lines of miRNC group and miR1291 group were 1.00±0.03 and 775.25±329.91 respectively, with a significant difference (t=4.694, P=0.003); and the expression levels of PHF8 mRNA were 1.00±0.11 and 0.57±0.18 respectively, with a significant difference (t=4.122, P=0.006). The results of Western blotting were consistent with the results of qRTPCR, and the expressions of CDK6 and Cyclin D1 were significantly decreased. The double luciferase reporter gene showed that miR1291 could directly inhibit the activity of luciferase in the 3′ untranslated region of target gene PHF8. Compared with miR-NC group, the proportion of renal carcinoma cells in S phase (23.40±4.29 vs. 32.19±2.64; t=3.491, P=0.013) and G2-M phase (14.38±4.05 vs. 25.59±6.01; t=3.095, P=0.021) decreased; and the proportion of cells in G0-G1 phase increased (62.22±7.56 vs. 42.22±5.23, t=4.351, P=0.005). MTT assay showed that the cell viability of miR1291 was significantly decreased. Colony formation experiments showed that the numbers of colonies formed by A498 cells in miR-NC group and miR1291 group were 246.64±39.94 and 87.34±21.93 respectively, with a significant difference (t=6.993, P<0.001). ConclusionThe expression of miR1291 is significantly decreased in renal cancer cell lines. miR1291 can significantly inhibit the proliferation of renal cell carcinoma cells by targeting interfering PHF8 gene expression, which may contribute to the development of new renal cancer target.
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    Effects of miR-1280 expression on the cell cycle and proliferation of bladder cancer by activating p21 gene expression
    Ye Zhihua, Huang Geng, Fu Jinlun, Gui Dingwen
    2018, 45 (3):  134-138.  doi: 10.3760/cma.j.issn.1673422X.2018.03.002
    Abstract ( 475 )   PDF (1086KB) ( 633 )   Save
    Objective To investigate the activation effect of microRNA1280 (miR1280) on the expression of p21 gene in bladder cancer cell line BIU87 and its effect on cell cycle and proliferation of bladder cancer cell line. MethodsRealtime fluorescence quantitative polymerase chain reaction (qRTPCR) was used to detect the expressions of miR1280 in bladder cancer cell lines T24, 5637, J82, BIU87 and normal bladder epithelial cells SVHUC1. miR1280 mimics (experimental group) and miRNC (control group) were transfected into the bladder cancer cells with the lowest expression of miR1280. The expressions of miR1280 and p21 mRNA were detected by qRTPCR. Chromatin immunoprecipitation (ChIP) was used to verify the targeting effect of miR1280 and p21 gene promoter. Western blotting was used to detect the expressions of p21, cell cycledependent kinase 1 (CDK1), Cyclin A2 mRNA and protein in the two groups. Cell cycle was detected by flow cytometry, and cell proliferation was detected by methyl thiazolyl tetrazclium (MTT) assay. ResultsThe results of qRTPCR indicated that the expression levels of miR1280 in bladder cancer cell lines T24, 5637, J82 and BIU87 and normal urothelium cell line SVHUC1 were 0.503±0.094, 0.611±0.054, 0.567±0.077, 0.257±0.032 and 1.014±0.090 respectively, with a significant difference (F=1.880, P<0.001). Compared with bladder cancer cell lines T24, 5637 and J82 cells, the expression of miR1280 in BIU87 cell was the lowest (P=0.026, P=0.003, P=0.008). Compared with the control group, the expression of miR1280 in BIU87 cell was significantly increased (1041.000±157.500 vs. 1.023±0.118, t=6.606, P<0.001), and the expression of p21 mRNA was also significantly increased (5.280±0.660 vs. 1.007±0.070, t=6.440, P<0.001). Western blotting showed that p21 protein expression was upregulated, CDK1 and Cyclin A2 protein expressions were downregulated. ChIP experiments showed that compared with the miRNC transfection group, the concentration of biotin modified miR1280 in the p21 gene promoter region was significantly increased (1.246±0.171 vs. 0.519±0.087, t=3.787, P=0.009). The proportion of G0G1 cells in the experimental group BIU87 cells was significantly higher than that in the control group (68.360%±3.064% vs. 46.970%±3.971%, t=4.263, P=0.005). The results of MTT showed that compared with the control group, the cell proliferation ability of BIU87 cells after being transfected miR1280 was significantly decreased starting from day 3 (0.826±0.099 vs. 1.224±0.057, t=3.505, P=0.013). ConclusionmiR1280 can activate the expression of p21 gene in bladder cancer cell line BIU87 by binding the promoter region of p21 gene, blocking the progression of cell cycle and inhibiting cell proliferation, which provides a new direction for bladder cancer targeted therapy theory.
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    Effects of miR-138 on invasion of brain glioma cells through targeting Sema4C
    Li Huibing, Yao Juan
    2018, 45 (3):  139-142.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.003
    Abstract ( 559 )   PDF (922KB) ( 764 )   Save
    ObjectiveTo explore the effects and mechanism of microRNA-138 (miR-138) on brain glioma cells invasion and migration. MethodsThe expression of miR138 was detected by real time quantitative polymerase chain reaction (qRT-PCR) in 60 cases of glioma tissues and paracarcinoma tissues, and the relationship between miR138 expression and glioma grading was analyzed. Human glioma cells line U87 and U251 were transfected with miR138 mimic and negative control (miR-NC). The expression of miR138 was detected by qRTPCR. The migration and invasion abilities were tested by Transwell assay, and the expression of semaphoring 4C (Sema4C) protein was tested by Western blotting. ResultsThe expression level of miR-138 in glioma tissues (2.46±1.07) was significantly lower than that in normal brain tissues (4.83±1.16, t=-11.631, P<0.001), and miR138 expression was negatively correlated with tumor grade (r=-0.563, P=0.001). The expression level of miR138 in cells was significantly higher after being transfected with miR-138 mimic (U251: 3.96±0.16; U87: 4.43±0.96) than miR-NC (U251: 2.32±0.36; U87: 2.58±0.62, t=7.253, P<0.001; t=8.872, P<0.001). The ability of invasion and migration were lower after being transfected with miR-138 mimic (U251: 89±9; U87: 95±10) than miR-NC (U251: 206±15; U87: 240±20, t=36.629, P<0.001; t=35.521, P<0.001). The expression of Sema4C protein was lower after being transfected with miR-138 mimic (U251: 0.41±0.06; U87: 0.36±0.03) than miRNC (U251: 1.01±0.08; U87: 1.03±0.13, t=-32.862, P<0.001; t=-27.512, P<0.001). ConclusionThe upregulated expression of miR138 can suppress glioma cells migration and invasion, which might be related to the negative regulation expression of Sema4C protein.
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    Diagnostic value of lymph node EBV-DNA detection in cervical lymph node metastasis of nasopharyngeal carcinoma
    Huang Can, Chen Qiuyan, Zuo Feifei, Peng Chuan, Zhong Shaobin, Mai Haiqiang, Chen Mingyuan, Zou Ruhai
    2018, 45 (3):  143-147.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.004
    Abstract ( 997 )   PDF (863KB) ( 831 )   Save
    Objective To evaluate the diagnostic value of lymph node fineneedle aspiration (FNA) EpsteinBarr virus (EBV)-DNA concentration detection in nasopharyngeal carcinoma (NPC) cervical lymph node metastasis. MethodsFrom August to December 2016, 36 cases of NPC and 9 cases of other tumors (not correlated with EBV infection) were enrolled in this study at the Sun Yatsen University Cancer Center. All patients received magnetic resonance images (MRI), plasma and cervical lymph node FNA EBVDNA detection. ResultsThe median concentration of EBVDNA in FNA fluid (1.39×105 copies/ml) in cervical lymph node metastasis was significantly higher than that in plasma (2.00×103 copies/ml), with a significant difference (χ2=16.723, P=0.004). The diagnosis sensitivity, specificity, accuracy of the lymph node FNA fluid of EBVDNA were 86.2% (25/29), 71.4% (10/14) and 81.4% (35/43) respectively, which were better than those of MRI [72.4% (21/29), 50.0% (7/14) and 65.1% (28/43) respectively] and plasma EBVDNA [55.2% (16/29), 71.4% (10/14) and 60.5% (26/43) respectively]. The area under the curve (AUC) of level Ⅰb cervical lymph node metastasis was calculated, and FNA fluid EBVDNA (AUC=0.688) was better than MRI (AUC=0.583), with a significant difference (Z=2.476, P=0.008). The EBVDNA concentration in FNA fluid in cervical lymph node metastasis of patients with other tumors (no correlated with EBV infection) was 0 copy/ml. ConclusionFNA fluid EBVDNA may improve the diagnostic sensitivity of cervical lymph node metastasis in nasopharyngeal carcinoma, and help to explore the clinical target volume neck nodes at level Ⅰb cervical lymph node in radiotherapy.
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    Influences of the size of lymph node metastasis on the chemoradiotherapy efficacy and prognosis for the patients after esophagectomy of thoracic esophageal squamous cell carcinoma
    Guo Xinwei, Ji Shengjun, Zhou Shaobing, Gu Liang, Liu Yangchen
    2018, 45 (3):  148-156.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.005
    Abstract ( 608 )   PDF (895KB) ( 1059 )   Save
    Objective To evaluate the effects of the size of lymph node metastasis (LNM) on the chemoradiotherapy efficacy and prognosis for the patients after resection of thoracic esophageal squamous cell carcinoma (ESCC). Methods Between 2011 and 2014, a total of 75 esophageal squamous carcinoma patients with secondary LNM after resection of ESCC were recruited in this retrospective study. They were treated with curative radiotherapy only or concurrent chemoradiotherapy in the Affiliated Taixing People′s Hospital of Yangzhou University. The LNM volume and maximum diameters were measured by the Monaco treatment planning system. The enrolled patients were grouped according to the median values of LNM volume and maximum diameters. The relationship between the responsiveness to treatment and these markers was analyzed by univariate and multivariate logistic analysis. The KaplanMeier method and Log-rank test were adopted to calculate and compare the overall survival (OS) rates with these markers. The Cox proportional hazards model was used to carry out univariate and multivariate analyses. Results The overall effective rate was 69.3% for all enrolled patients. The response rates were 81.6% with LNM volume <57cm3 and 56.8% with LNM volume ≥57cm3. The response rates were 83.8% with LNM maximum diameter <5cm and 55.3% with LNM maximum diameter ≥5cm. The responses to treatment were highly associated with treatment method (OR=1.825, 95%CI: 1.1343.658, P=0.017), LNM volume (OR=4.183, 95%CI: 1.41612.354, P=0.010) and maximum diameter (OR=3.374, 95%CI: 1.1859.611, P=0.023) by univariate logistic regression analysis. Multivariate logistic regression analysis showed that therapeutic method (OR=1.225, 95%CI: 1.0852.837, P=0.038) and LNM volume (OR=1.614, 95%CI: 1.0033.025, P=0.048) were independent risk factors for tumor response. The median OS time of this cohort was 14 months, and the 1, 2 and 3 year OS rates were 60.7%, 25.3% and 20.1%, respectively. KaplanMeier survival analysis revealed that TNM stage (HR=2.039, 95%CI: 1.2343.370, P=0.005), treatment methods (HR=1.858, 95%CI: 1.3852.958, P=0.013), LNM volume (HR=2.642, 95%CI: 1.5524.497, P<0.001) and LNM maximum diameter (HR=3.399, 95%CI: 1.9395.958, P<0.001) were significantly associated with OS. Furthermore, multivariate Cox proportional hazard regression model analysis for OS was performed and the results showed that TNM stage (HR=2.023, 95%CI: 1.1493.560, P=0.015), LNM volume (HR=2.055, 95%CI: 1.0414.055, P=0.038) and maximum diameter (HR=1.910, 95%CI: 1.1373.895, P=0.045) were considered as independent prognostic risk factors for OS. Conclusion LNM volume in ESCC patients with secondary LNM after esophagectomy has great values for predictive therapeutic effects and survival outcomes, and LNM maximum diameter has significant value for survival outcomes.
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    Expressions and clinical significances of CD68 and ABHD5 in gastric carcinoma
    Yao Luyan, Wang Zian
    2018, 45 (3):  153-156.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.006
    Abstract ( 530 )   PDF (968KB) ( 768 )   Save
    Objective To evaluate the expressions of CD68 and alpha/beta hydrolase domaincontaining protein 5 (ABHD5) in gastric carcinoma and their interrelation, and the relationship with the clinicopathological features of gastric carcinoma. Methods The immunohistochemistry staining was used to determine the expressions of CD68 and ABHD5 in 60 cases of gastric carcinoma and 30 cases of adjacent normal tissues. The relationships between CD68, ABHD5 and clinicopathological features of gastric carcinoma, and the correlation of them were analyzed. Results The expressions of CD68 and ABHD5 in gastric carcinoma were significantly higher than those in adjacent normal tissues, and the positive expression rates were 65.00% vs. 26.67% (χ2=11.779, P=0.001) and 60.00% vs. 30.00% (χ2=7.200, P=0.007) respectively, with significant differences. The expression of CD68 was related to TNM stage (χ2=7.959, P=0.005), lymph node metastasis (χ2=6.901, P=0.009) and serosal invasion (χ2=5.833, P=0.016), but it was not associated with pathological grade (χ2=0.028, P=0.866), gender (χ2=0.533, P=0.465) and age (χ2=0.060, P=0.807). The expression of ABHD5 was related to pathological grade (χ2=4.318, P=0.038), TNM stage (χ2=4.051, P=0.044) and serosal invasion (χ2=5.275, P=0.022), but it was not associated with lymph node metastasis (χ2=0.545, P=0.460), gender (χ2=0.191, P=0.662) and age (χ2=1.358, P=0.244). Contingency correlation analysis showed that there was positive correlation between CD68 and ABHD5 in gastric cancer (c=0.328, P=0.010). Conclusion The expressions of CD68 and ABHD5 in gastric carcinoma are higher than those in adjacent normal tissues, which are correlated with the different clinicopathological features of gastric carcinoma. The expression of CD68 is positively correlated with the expression of ABHD5. The combined detection of CD68 and ABHD5 expressions in gastric carcinoma is helpful to evaluate the invasion, metastasis and prognosis of gastric carcinoma.
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    Relationship between total serum IgG concentration and tumor progression in patients with gastric cancer
    Wei Tiejun, Guo Yijing, Zhang Yongli, Jiang Meng, Zhang Heng
    2018, 45 (3):  157-160.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.007
    Abstract ( 669 )   PDF (717KB) ( 703 )   Save
    Objective To investigate the relationship between total serum IgG concentration and tumor progression in patients with gastric cancer. Methods From January 2010 to May 2017, a total of 90 patients with gastric cancer in Sun Simiao Hospital of Beijing University of Chinese Medicine were enrolled as study subjects and 90 ageandsex-matched adults from normal physical examination were enrolled as control subjects. The serum IgG subgroup and total IgG concentrations were measured by enzymelinked immunosorbent assay. The relationships between serum IgG concentration in patients with gastric cancer and clinicopathologic features were analyzed. The numbers of plasma cells with CD138 antibody in both tumor and non-cancerous tissues were evaluated by immunohistochemistry. Results The total serum IgG concentration in patients with gastric cancer was significantly lower than that in healthy control group [(923.6±290.5) mg/dl vs. (1072.5±298.6) mg/dl], with a significant difference (t=3.391, P=0.001). The total serum IgG and IgG1 concentrations were correlated with lymphatic metastasis (t=3.585, P<0.001; t=3.545, P<0.001) and gastric cancer stages (t=3.669, P<0.001; t=3.401, P<0.001). Furthermore, the CD138+ plasma cells in tumor tissues were significantly fewer than those in noncancerous gastric mucosa (88.7±31.9 vs. 108.5±33.4), with significant differences (t=2.778, P=0.007). Multivariate analysis suggested that total serum IgG concentration (HR=0.411, 95%CI: 0.0940.989, P=0.011) and lymph node metastasis (HR=3.148, 95%CI: 1.9885.297, P<0.001) were independent predictors for poor prognosis. Conclusion Decreased total serum IgG concentration is closely related to tumor progression and poor prognosis in patients with gastric cancer, which indicates that the impaired humoral immunity is associated with tumor progression.
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    Effects of Dingkoulizhong decoction on cellular immune factors and adverse reactions in patients with advanced gastric cancer treated with chemotherapy
    Yang Aiwu, Lin Zhimin, Zhang Jiangling, Liang Renpei, Zheng Weibin
    2018, 45 (3):  161-165.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.008
    Abstract ( 709 )   PDF (645KB) ( 692 )   Save
    Objective To investigate the effects of Dingkoulizhong decoction on cellular immune factors and adverse reactions in patients with advanced gastric cancer treated with chemotherapy. MethodsOne hundred and thirtyeight patients with advanced gastric cancer were enrolled in our hospital from October 2014 to December 2016, and were randomly divided into control group (n=69) and observation group (n=69) by using the random number table. The patients of the control group were treated with FOLFOX4 chemotherapy (oxaliplatin + calcium folinate + fluorouracil), while the patients of the observation group were given by the treatment of Dingkoulizhong decoction on the basis of the control group. The peripheral blood samples of the patients were collected before and after the treatment. The levels of cellular immune factors CD3+, CD4+, CD8+ and CD4+/CD8+ were detected, and the clinical efficacy and adverse reactions of the patients of the two groups were observed. Results After the treatment, the number of complete remission (CR), partial remission (PR), stable disease (SD) and progressive disease (PD) in the observation group were 16, 25, 16 and 12 cases respectively, while the control group were 9, 20, 19 and 21 cases respectively, and there was a statistically significant difference in the distribution of clinical efficacy between the two groups (Z=4.381, P=0.036). Compared with the control group, the clinical benefit rate (CBR) of the observation group was significantly improved (59.42% vs. 42.03%), with a statistically significant difference (χ2=4.175, P=0.041). The cellular immune factors CD3+[(52.67±6.21)% vs. (53.45±6.54)%], CD4+[(23.56±3.85)% vs. (24.09±2.91)%], CD8+[(28.16±3.49)% vs. (27.87±3.26)%] and CD4+/CD8+ (1.13±0.27 vs. 1.19±0.31) of the patients of the observation group showed no statistically significant difference (t=0.718, P=0.474; t=0.912, P=0.363; t=0.504, P=0.615; t=1.212, P=0.227) before and after the treatment, but the cellular immune factor CD3+[(50.36±3.74)% vs. (53.13±6.12)%], CD4+[(21.26±2.37)% vs. (23.44±3.96)%] and CD4+/CD8+ (0.96±0.26 vs. 1.15±0.25) of the patients of the control group after the treatment were significantly lower than those before the treatment, and CD8+[(31.64±4.05)% vs. (27.98±3.52)%] after the treatment was significantly higher than that before the treatment, all with statistically significant differences (t=3.208, P<0.001; t=3.924, P<0.001; t=4.289 P<0.001, t=5.666, P<0.001). Compared with the control group, the level of CD3+[(53.45±6.54)% vs. (50.36±3.74)%], CD4+[(24.09±2.91)% vs. (21.26±2.37)%] and CD4+/CD8+ (1.19±0.31 vs. 0.96±0.26) of the patients of the observation group after the treatment were significantly improved, and CD8+[(27.87±3.26)% vs. (31.64±4.05)%] was significantly decreased, all with statistically significant differences (t=3.407, P=0.001; t=6.264, P<0.001; t=4.722, P<0.001; t=6.023, P<0.001). Compared with the control group, the total adverse reaction rate of the observation group was significantly decreased (36.23% vs. 55.07%), with a statistically significant difference (χ2=4.936, P=0.026). Conclusion Dingkoulizhong decoction can significantly improve the clinical efficacy in patients with advanced gastric cancer treated with chemotherapy, alleviate the immune function damage caused by chemotherapy, and it can reduce the occurrence of adverse reactions.
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    Role of human leukocyte antigenDR expression on predicting the prognosis of hepatocellular carcinoma
    Wu Quanlin, He Yaopeng
    2018, 45 (3):  166-171.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.009
    Abstract ( 654 )   PDF (900KB) ( 701 )   Save
    Objective To explore the expression of human leukocyte antigen DR (HLA-DR) in hepatocellular carcinoma and its clinical values in predicting prognosis. Methods The primary hepatocellular carcinoma tissues of 97 patients in our hospital from August 2012 to February 2014 were enrolled as subjects (case group), and the adjacent normal liver tissues were selected as control (control group, n=97). The expressions of HLADR in the two groups were detected by immunohistochemistry, and the relationships between HLADR expression and clinicopathological features, recurrence and prognosis were analyzed. Results The positive expression rate of HLADR in specimens of case group was 55.67%, which was higher than 21.65% in control group, and the difference was statistically significant (χ2=23.671, P<0.001). The positive expression rates of HLADR in tumor tissues with diameter ≥4cm, Ⅲ-Ⅳ stage, poorly differentiated, bile duct cell carcinoma, no lymph node metastasis were higher than those in tumor tissues with diameter <4cm, Ⅰ-Ⅱ stage, medium and well differentiated, hepatocellular carcinoma, lymph node metastasis respectively, and the differences were statistically significant (χ2=10.481, P=0.001; χ2=18.854, P<0.001; χ2=9.876, P=0.002; χ2=6.834, P=0.009; χ2=30.668,P<0.001). The recurrence rates of 2 years and 3 years were 27.91% and 46.51% in patients with negative expression of HLADR respectively, which were lower than 50.00% and 70.37% in patients with positive expression of HLADR respectively, and the differences were statistically significant (χ2=4.860, P=0.028; χ2=5.668, P=0.017). The median survival time of patients with HLADR positive expression was 33.64 months, which was shorter than 36.88 months in patients with negative expression, and the difference was statistically significant (χ2=10.356, P<0.001). TNM staging Ⅲ-Ⅳ (OR=0.899, 95%CI: 0.721-0.995, P=0.012) and positive expression of HLADR (OR=1.125, 95%CI: 1.025-2.568, P=0.018) were unfavorable to the prognosis of hepatocellular carcinoma patients. Conclusion HLA-DR expresses abnormally in patients with primary hepatocellular carcinoma, which may be involved in the occurrence and development of hepatocellular carcinoma, and the upregulation of HLA-DR in patients with hepatocellular carcinoma indicates a high recurrence rate and short survival time.
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    Anticancer mechanism and application of curcumin analog EF24
    Ling Xiaofei, Xu Ke, Shao Hua, Wang Cuijuan
    2018, 45 (3):  172-175.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.010
    Abstract ( 516 )   PDF (639KB) ( 782 )   Save
    Curcumin is a polyphenol pigment product from the rhizome of curcumin longa. It is associated with numerous therapeutic benefits, including antioxidant, antiinflammatory and anticancer activities. Research shows that EF24 is a curcumin analog that can induce tumor cell apoptosis, arrest cell cycle, inhibit cell invasion and metastasis and has synergistic effect of combined antitumor agents. EF24 has improved anticancer activity and bioavailability against various cancer cell lines over curcumin without increased toxicity. EF24 would have great potential to be used as a useful therapeutic agent for the treatment of various types of cancer.
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    Several problems about the stereotactic body radiation therapy for lung cancer
    Su Chuanghuang, Li Derui
    2018, 45 (3):  176-179.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.011
    Abstract ( 518 )   PDF (638KB) ( 662 )   Save
    As the first treatment of choice for medically inoperable patients with early stage nonsmall cell lung cancer, it is demonstrated that the therapeutic outcome of stereotactic body radiation therapy (SBRT) is similar to that of surgery, but the data is mainly from nonrandomized controls and retrospective studies. More cases of randomized prospective clinical trials are needed to confirm the conclusions. The biologically effective dose >100Gy is associated with improved local control and overall survival, and the small tumor volume is correlated with higher local control rate.
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    Research progress on liquid biopsy in pancreatic cancer
    Li Zhijian, He De
    2018, 45 (3):  180-182.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.012
    Abstract ( 539 )   PDF (687KB) ( 781 )   Save
    Liquid biopsy is a kind of popular and emerging pathological detection technology, which mainly includes the detection of circulating tumor cells, circulating tumor DNA and exosome. Recent studies show that liquid biopsy not only plays a potential advantage in early diagnosis of pancreatic cancer, but also has some guiding significance for prognosis and recurrence monitoring of pancreatic cancer.
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    Diabetes, hypoglycemic drugs and pancreatic cancer
    Liang Biyu, He Weiming, He Xiaoyi, Ding Yuanlin, Yu Haibing
    2018, 45 (3):  183-186.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.013
    Abstract ( 613 )   PDF (691KB) ( 872 )   Save
    About 80% of the patients with pancreatic cancer have glucose metabolism alterations, which suggests an association between diabetes and the occurrence and development of pancreatic cancer. Studies have shown that diabetes and hypoglycemic agents are closely related to the occurrence risk, clinical manifestation and prognosis of pancreatic cancer. Recent findings demonstrate that insulin resistance, hyperinsulinemia, the expression level of insulinlike growth factor related protein and inflammatory reaction are the possible mechanisms of the interaction between diabetes and pancreatic cancer. However, the definite mechanism still remains unclear, and further researches are still needed.
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    Characteristics of diffuse large Bcell lymphoma in Chinese populations
    Zhang Qian, Duan Shan
    2018, 45 (3):  187-190.  doi: 10.3760/cma.j.issn.1673-422X.2018.03.014
    Abstract ( 657 )   PDF (638KB) ( 1432 )   Save
    Diffuse large Bcell lymphoma (DLBCL) is the most common type of nonHodgkin lymphoma. Generally, it can be divided into two immunological subgroups: germinal center Bcelllike (GCB) and nongerminal center Bcelllike (nonGCB), and they are different both in distribution and prognosis in different populations. And DLBCL has unique genetic characteristics in Chinese populations. With the global advances in personalized medicine, people gradually realize that only personalized therapy could be obtain maximal therapeutic effect for different populations, especially person from different kind of ethnic group.
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