国际肿瘤学杂志

国际肿瘤学杂志 ›› 2012, Vol. 39 ›› Issue (10): 797-800.

• 论著 • 上一篇    

和厚朴酚对人急性白血病细胞株U937细胞增殖及凋亡的影响

刘淑娟,范华,姜国胜   

  1. 250011济南，山东中医药大学附属医院护理部（刘淑娟）；山东省医学科学院基础医学研究所 山东省罕少见病重点实验室 卫生部生物技术药物重点实验室 国家中医药管理局免疫药理（三级）实验室（范华 、姜国胜）
  • 出版日期:2012-10-31 发布日期:2012-10-31
  • 通讯作者: 姜国胜，E-mail: jiangguosh@163.com E-mail:jiangguosh@163.com
  • 基金资助:

    国家自然科学基金资助项目(30771103）

Effects of honokiol on the proliferation and apoptosis of human acute leukemia U937 cells

 LIU  Shu-Juan, FAN  Hua, JIANG  Guo-Sheng   

  1. Nursing Department， Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China
  • Online:2012-10-31 Published:2012-10-31
  • Contact: JIANG Guo-sheng， E-mail: jiangguosh@163.com E-mail:jiangguosh@163.com

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摘要： 目的 探讨和厚朴酚在体外对人急性白血病细胞株U937细胞增殖及凋亡的影响.方法 采用四甲基偶氮唑蓝（MTT）法检测和厚朴酚对细胞增殖的影响，Annexin V/PI双染检测细胞凋亡，RT-PCR检测Bcl-2、Bax、Caspase 3、Caspase 8 和 Caspase 9基因的表达。结果 5 μg/ml的和厚朴酚作用48 h对U937细胞增殖即可表现抑制作用，10 μg/ml的和厚朴酚作用48 h对细胞的增殖抑制率高达50%以上，作用120 h可完全抑制细胞增殖。经10 μg/ml和厚朴酚处理的U937细胞凋亡率达到26.8% (P＜0.01)。10 μg/ml的和厚朴酚处理U937细胞后48 h，Bcl-2基因表达降低（对照组：0.33±0.02，实验组：0.14±0.01，P＜0.01），Bax基因表达升高（对照组：0.1±0.01，实验组：0.87±0.08，P＜0.01）。Caspase 3（对照组：0.48±0.01，实验组：0.87±0.06，P＜0.01）、Caspase 8（对照组：0.23±0.02，实验组：0.41±0.07，P＜0.01）和Caspase 9（对照组：0.44±0.05，实验组：0.76±0.06，P＜0.01）基因表达均升高。Caspase 3活性为0.325±0.089，较对照组有显著地升高(P＜0.01.结论 和厚朴酚能明显抑制人急性白血病细胞株U937细胞增殖并引起细胞凋亡。其机制在于上调凋亡促进基因Bax的表达，下调凋亡抑制基因Bcl2的表达，并且内源性和外源性途径均参与了凋亡过程。

关键词: 和厚朴酚, 白血病, U937细胞, 细胞增殖, 细胞凋亡

Abstract: Objective To detect the mechanism of the growth inhibition and apoptosis of human acute leukemia cell line U937 cells induced by honokiol. Metheds The proliferation inhibition was detected by MTT method. Cell apoptosis was tested by Hoechst 33342 staining and flow cytometry with Annexin V/PI staining. RT-PCR was used to detect the mRNA expression of the apoptosis gene Bcl-2,Bax,Caspase 3,Caspase 8 and Caspase 9.Results The inhibition effect of honokiol(5 μg/ml, 48 h) on U937 cells proliferation could be observed, and the inhibition rate of 10 μg/ml honokiol on cell proliferation reached above 50%(48 h). U937 cells proliferation could be completely inhibited for 120 h. U937 cells apoptosis rate reached 26.8% (P＜0.01) after being treated with 10 μg/ml honokiol. After being treated with 10 μg/ml honokiol for 48 h, the Bcl-2 gene expression in U937 cells was reduced (control group: 0.33±0.02, experimental group: 0.14±0.01,P＜0.01), and the Bax gene expression was elevated (control group: 0.1±0.01, experimental group: 0.87±0.08,P<0.01). The gene expressions of Caspase 3(control group: 0.48±0.01, experimental group: 0.87±0.06, P＜0.01), Caspase 8(control group: 0.23±0.02, experimental group: 0.41±0.07, P＜0.01) and Caspase 9(control group: 0.44±0.05, experimental group: 0.76±0.06, P＜0.01) were all increased. The activity of Caspase 3 was 0.325±0.089, which was significantly higher than that of the control group ( P＜0.01 ).Conclusion Honokiol can significantly inhibit the proliferation and induce cell apoptosis of human acute leukemia cell line U937 cells. The mechanism is relatedto the upregulationof Bax and downregulation of Bcl-2, and the endogenous and exogenous pathways are both involved in the apoptosis process.

Key words: Honokiol , Leukemia, U937 cells, Cell proliferation, Cell apoptosis