X线诱导人鼻咽鳞状细胞癌CNE1细胞株MDR-1及P-gp的表达研究

doi:10.3760/cma.j.issn.1673-422X.2018.06.001

摘要/Abstract

摘要： 目的检测X线照射前后鼻咽鳞状细胞癌CNE1细胞株中多药耐药基因（MDR）-1及P糖蛋白（P-gp）的表达情况。方法对CNE1细胞进行X线照射，在X线照射后、CNE1细胞培养24 h后进行检测，半定量反转录聚合酶链反应（RTPCR）检测X线照射前后CNE1细胞中MDR-1 mRNA的表达；Western blotting检测X线照射前后CNE1细胞中Pgp的蛋白表达；共聚焦显微镜观察照射前后CNE1细胞中P-gp的蛋白表达情况。结果 RT-PCR检测结果显示，X线照射前后CNE1细胞中MDR1 mRNA的吸光度（A）值分别为0.17±0.01和0.34±0.03，差异具有统计学意义（t=16.541，P＜0.001）。Western blotting检测结果显示，X线照射前后CNE1细胞中P-gp蛋白的A值分别为0.02±0.01和0.04±0.01，差异具有统计学意义（t=4.612，P=0.016）。共聚焦显微镜观察X线照射后CNE1细胞中P-gp的蛋白表达绿色荧光强度高于照射前。结论 X线照射可引起CNE1细胞中MDR-1及P-gp表达的升高，提示X线照射可使鼻咽鳞状细胞癌CNE1细胞产生多药耐药性。

关键词: 鼻咽肿瘤, CNE1细胞, 抗药性, 多药, X射线照射

Abstract: Objective To examine the expressions of multidrug resistance gene (MDR)-1 and P-glycoprotein (P-gp) in nasopharyngeal squamous cell carcinoma CNE1 cell line before and after X-ray exposure. Methods CNE1 cells were exposed to X-ray. After the irradiation, the CNE1 cells were cultured for 24 hours and tested. The mRNA expressions of MDR-1 in CNE1 cells were measured by semiquantitative realtime polymerase chain reaction (RT-PCR) before and after X-ray exposure, and the protein expressions of P-gp in CNE1 cells were detected by Western blotting. The protein expressions of Pgp in CNE1 cells were observed by confocal microscope before and after X-ray exposure. Results The results of RT-PCR showed that the absorbance (A) values of MDR1 mRNA in CNE1 cells were 0.17±0.01 and 0.34±0.03 before and after irradiation, and the difference was statistically significant (t=16.541, P＜0.001). The results of Western blotting showed that the A values of P-gp protein in CNE1 cells were 0.02±0.01 and 0.04±0.01, and the difference was statistically significant (t=4.612, P=0.016). The green fluorescence intensity of P-gp protein in CNE1 cells after X-ray irradiation was higher than that before X-ray irradiation by confocal microscope. Conclusion X-ray irradiation can cause the high expressions of MDR-1 and P-gp in CNE1 cells, suggesting that X-ray irradiation can induce the occurrence of multidrug resistance in CNE1 cells.

Key words: Nasopharyngeal neoplasms, CNE1 cells, Drug resistance, multiple, X-ray irradiation

皇婷, 宋学薇, 安书芬, 王志斌, 廖和和. X线诱导人鼻咽鳞状细胞癌CNE1细胞株MDR-1及P-gp的表达研究[J]. 国际肿瘤学杂志, 2018, 45(6): 321-324.

Huang Ting, Song Xuewei, An Shufen, Wang Zhibin, Liao Hehe. Expressions of MDR-1 and P-gp in human nasopharyngeal squamous cell carcinoma CNE1 cell line after X-ray exposure[J]. Journal of International Oncology, 2018, 45(6): 321-324.

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