国际肿瘤学杂志

国际肿瘤学杂志 ›› 2017, Vol. 44 ›› Issue (6): 415-.doi: 10.3760/cma.j.issn.1673-422X.2017.06.004

• 论著 • 上一篇    下一篇

HIF-1α和CA9在乳腺癌中的表达及其与基底细胞样型乳腺癌的关系

孙吉瑞,周炳娟,陈红,马秋双,张金库,孙哲,张东晨   

  1. 071000 保定市第一中心医院病理科（孙吉瑞、周炳娟、陈红、马秋双、张金库），护理部（孙哲），皮肤科
    （张东晨）
  • 出版日期:2017-06-08 发布日期:2017-06-16
  • 通讯作者: 张金库 E-mail:zjkblk@sohu.com

Expressions of HIF-1α and CA9 in breast carcinoma and their relationships with basal-like subtype

Sun Jirui, Zhou Bingjuan, Chen Hong, Ma Qiushuang, Zhang Jinku, Sun Zhe, Zhang Dongchen   

  1. Department of Pathology, First Central Hospital of Baoding, Hebei 071000, China
  • Online:2017-06-08 Published:2017-06-16
  • Contact: Zhang Jinku E-mail:zjkblk@sohu.com

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摘要： 目的探讨缺氧诱导因子1α（HIF1α）和碳酸酐酶9（CA9）在乳腺癌各分子亚型中的表达及其与基底细胞样型乳腺癌的关系。方法采用免疫组织化学法检测HIF1α和CA9在5种不同乳腺癌亚型(共803例)中的表达水平并分析其与基底细胞样型乳腺癌临床病理特征及患者生存时间的关系。结果HIF1α和CA9在乳腺癌组织中的阳性表达率显著高于癌旁乳腺组织（43.96%∶3.86%，χ2=354.858，P<0.001；19.80%∶4.61%，χ2=86.495，P<0.001）。管腔A型、管腔B型、人表皮生长因子受体2（HER2）过表达型、基底细胞样型、普通乳腺样型中HIF1α的阳性表达率分别为31.97%、25.00%、56.06%、77.89%和55.76%，CA9的阳性表达率分别为7.48%、5.56%、60.61%、40.00%和27.88%，差异均具有统计学意义（χ2=88.628，P＜0.001；χ2=147.128，P＜0.001）；HIF1α和CA9在雌激素受体（ER）阴性的HER2过表达型、基底细胞样型和普通乳腺样型中的表达明显高于ER阳性的管腔A型和管腔B型（均P<0.005）。HIF1α和CA9的表达与基底细胞样型乳腺癌的淋巴结转移（χ2=4.542，P=0.033；χ2=7.025，P=0.008）、pTNM分期（χ2=5.390，P=0.020；χ2=10.179，P=0.001）及不良预后有关（χ2=4.353，P=0.037；χ2=6.056，P=0.014），且二者在基底细胞样型乳腺癌中的表达具有正相关关系（C=0.315，P=0.001）。结论HIF1α和CA9过表达可能与ER阴性乳腺癌关系更密切，二者可能协同在基底细胞样型乳腺癌的发生、发展中发挥重要作用。基底细胞样型乳腺癌HIF1α和CA9过表达提示患者预后不良。

关键词: 乳腺肿瘤, 缺氧诱导因子1, &alpha, 亚基, 碳酸酐酶类, 基底细胞样型

Abstract: ObjectiveTo study the expressions of hypoxia inducible factor1α (HIF1α) and carbonic anhydrase 9 (CA9) in different molecular subtypes of breast carcinoma and their relationships with basallike subtype. MethodsThe expressions of HIF1α and CA9 were detected by immunohistochemistry in five subtypes of breast carcinoma （n=803）. The relationships of the expressions of HIF1α and CA9 with the clinicopathologic parameters of basallike subtype and with the prognosis of patients were analyzed. ResultsThe positive expression rates of HIF1α and CA9 protein in breast carcinoma were much higher than those in paratumor breast tissues (43.96% vs. 3.86%, χ2=354.858, P<0.001; 19.80% vs. 4.61%, χ2=86.495, P<0.001). The positive expression rates of HIF1α in Luminal A, Luminal B, epidermal growth factor receptor2 (HER2) overexpression, basallike and normal breastlike subtypes were 31.97%, 25.00%, 56.06%, 77.89% and 55.76% respectively, and the positive expression rates of CA9 were 7.48%, 5.56%, 60.61%, 40.00% and 27.88% respectively, with significant differences (χ2=88.628, P＜0.001; χ2=147.128, P＜0.001). The positive expression rates of HIF1α and CA9 in estrogen receptor (ER)negative breast carcinomas (HER2 overexpression, basallike and normal breastlike) were significantly higher than those in ERpositive subtypes (Luminal A and Luminal B, all P<0.005). There was a significant positive correlation between HIF1α and CA9 (C=0.315, P=0.001), and both were correlated with the lymph node metastasis (χ2=4.542, P=0.033; χ2=7.025, P=0.008), pTNM stage (χ2=5.390, P=0.020; χ2=10.179, P=0.001) and poor prognosis (χ2=4.353, P=0.037; χ2=6.056, P=0.014) of basallike subtype. ConclusionOverexpressions of HIF1α and CA9 may be related to the ERnegative breast cancer more closely, and both may play important roles in the occurrence and development of basallike subtype. Overexpressions of HIF1α and CA9 may suggest the poorer prognosis of patients with basallike breast carcinoma.

Key words: Breast neoplasms, Hypoxiainducible factor 1, alpha subunit, Carbonic anhydrases, Basallike subtype