国际肿瘤学杂志

国际肿瘤学杂志 ›› 2025, Vol. 52 ›› Issue (2): 89-93.doi: 10.3760/cma.j.cn371439-20240727-00013

• 论著 • 上一篇    下一篇

MRI联合血清lncRNA KCNQ1OT1、miR-204-5p对乳腺癌腋窝淋巴结转移的预测价值

王智宝1(), 李广现1, 张昕昕1, 崔伟2, 张微3   

  1. 1河北省保定市第二医院影像科，保定 071051
    2河北省保定市第二医院肿瘤外科，保定 071051
    3河北省保定市第二医院超声科，保定 071051
  • 收稿日期:2024-07-27 修回日期:2024-12-27 出版日期:2025-02-08 发布日期:2025-03-17
  • 通讯作者: 王智宝 E-mail:wangzhibao1985w@163.com
  • 基金资助:
    保定市科技计划(2141ZF196)

Predictive value of MRI combined with serum lncRNA KCNQ1OT1， miR-204-5p for axillary lymph node metastasis of breast cancer

Wang Zhibao1(), Li Guangxian1, Zhang Xinxin1, Cui Wei2, Zhang Wei3   

  1. 1Department of Imaging， No.2 Hospital of Baoding， Hebei Province， Baoding 071051， China
    2Department of Tumor Surgery， No.2 Hospital of Baoding， Hebei Province， Baoding 071051， China
    3Department of Ultrasound， No.2 Hospital of Baoding， Hebei Province， Baoding 071051， China
  • Received:2024-07-27 Revised:2024-12-27 Online:2025-02-08 Published:2025-03-17
  • Contact: Wang Zhibao E-mail:wangzhibao1985w@163.com
  • Supported by:
    Baoding Science and Technology Project(2141ZF196)

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摘要：

目的 探讨MRI联合血清长非编码RNA（lncRNA）KCNQ1OT1、微RNA-204-5p（miR-204-5p）预测乳腺癌患者腋窝淋巴结转移的价值。方法 选取2020年1月—2022年12月在河北省保定市第二医院手术并经病理诊断为乳腺癌的70例患者为研究对象，依据淋巴结转移状态分为转移组（n=31）和未转移组（n=39）。比较两组的MRI特征及血清lncRNA KCNQ1OT1、miR-204-5p水平；采用受试者操作特征（ROC）曲线评估三者单独及联合检测对乳腺癌腋窝淋巴结转移的预测效能。结果 转移组和未转移组乳腺癌患者肿瘤最大径（χ2=4.28，P=0.039）和Ki-67表达情况（χ2=10.88，P=0.001）差异均有统计学意义。转移组和未转移组患者的内部强化类型（χ2=6.60，P=0.037）、周围血管管径（t=4.33，P<0.001）、周围血管根数（t=4.38，P<0.001）、表观扩散系数（t=3.59，P=0.001）以及MRI淋巴结状态（χ2=29.70，P<0.001）差异均有统计学意义。转移组血清lncRNA KCNQ1OT1水平高于未转移组（1.41±0.32比0.99±0.18，t=6.94，P<0.001），miR-204-5p水平低于未转移组（0.72±0.17比1.03±0.21，t=6.66，P<0.001）。生物信息学分析显示，miR-204-5p可能与lncRNA KCNQ1OT1存在靶向关系。ROC曲线分析显示，MRI特征、血清lncRNA KCNQ1OT1、miR-204-5p水平和三者联合检测预测乳腺癌患者腋窝淋巴结转移的曲线下面积分别为0.823、0.858、0.843、0.946，三者联合检测的预测效能优于MRI特征、血清lncRNA KCNQ1OT1、miR-204-5p水平单独检测（Z=3.29，P=0.001；Z=2.07，P=0.038；Z=2.23，P=0.026）。结论 乳腺癌腋窝淋巴结转移患者血清lncRNA KCNQ1OT1水平上调，miR-204-5p水平下调，且MRI特征联合血清lncRNA KCNQ1OT1、miR-204-5p预测乳腺癌患者淋巴结转移的效能较高。

关键词: 乳腺肿瘤, 肿瘤转移, 磁共振成像, 长非编码RNA, KCNQ1OT1, miR-204-5p

Abstract:

Objective To investigate the value of MRI combined with serum long non-coding RNA （lncRNA） KCNQ1OT1 and microRNA-204-5p （miR-204-5p） in predicting axillary lymph node metastasis in breast cancer patients. Methods A total of 70 patients with breast cancer who were surgically diagnosed in the No.2 Hospital of Baoding， Hebei Province from January 2020 to December 2022 were selected as the study objects. Patients were divided into metastatic group （n=31） and non-metastatic group （n=39） according to the status of lymph node metastasis. The MRI features and the levels of serum lncRNA KCNQ1OT1 and miR-204-5p were compared between the two groups. Receiver operator characteristic （ROC） curve was used to evaluate the predictive efficacy of the three detections alone and in combination for axillary lymph node metastasis in breast cancer. Results There were statistically significant differences in tumor maximum diameter （χ2=4.28， P=0.039） and Ki-67 expression （χ2=10.88， P=0.001） between the metastatic and non-metastatic groups. There were statistically significant differences between the breast cancer axillary lymph node metastatic group and the non-metastatic group in the mode of internal reinforcement type （χ2=6.60，P=0.037）， peripheral vessel diameter（t=4.33，P<0.001）， number of peripheral vessel roots（t=4.38，P<0.001）， apparent diffusion coefficient（t=3.59，P=0.001）， and MRI lymph node status（χ2=29.70，P<0.001）. The level of serum lncRNA KCNQ1OT1 in metastatic group was higher than that in non-metastatic group （1.41±0.32 vs. 0.99±0.18， t=6.94， P<0.001）， and the level of miR-204-5p was lower than that in non-metastatic group （0.72±0.17 vs. 1.03±0.21， t=6.66， P<0.001）. Bioinformatics analysis showed that miR-204-5p may have a targeting relationship with lncRNA KCNQ1OT1. ROC curve analysis showed the area under the curve of MRI features， serum levels of lncRNA KCNQ1OT1， miR-204-5p and their combined detection to predict lymph node metastasis of breast cancer patients were 0.823， 0.858， 0.843， 0.946， respectively. The predictive efficacy of the combined detection was better than that of MRI features and serum lncRNA KCNQ1OT1 and miR-204-5p levels alone （Z=3.29， P=0.001； Z=2.07， P=0.038； Z=2.23， P=0.026）. Conclusions Serum lncRNA KCNQ1OT1 level is up-regulated and the level of miR-204-5p is down-regulated in patients with axillary lymph node metastasis of breast cancer. And the combination of MRI features with serum lncRNA KCNQ1OT1 and miR-204-5p has high predictive efficacy in predicting lymph node metastasis in breast cancer patients.

Key words: Breast neoplasms, Neoplasm metastasis, Magnetic resonance imaging, Long non-coding RNA, KCNQ1OT1, miR-204-5p