Abstract: Several molecular biomarkers (p16INK4a, Ki-67, and so on) are abnormally expressed in cervical precancerous lesions, many of which are involved in human papilloma virus (HPV)-induced molecular alterations. Cervical intraepithelial neoplasia (CIN) can not accurately predict the possibility of progress in precancerous lesions. Atypical squamous cells of undetermined significance (ASCUS) and low-grade squamous intraepithelial lesions (LSIL) are often over-treated clinically, while it lacks accurate screening methods to identify the precancerous lesions with high possibility of progression. Combining cytology diagnosis with biomarkers can help predict the progress of precancerous lesions and distinguish benign lesions which are difficult to identify morphologically. It appears promising to consider biomarkers as potential cervical precancerous lesions screening method.

Key words: Cervical intraepithelial neoplasia, Biomakers, p16^INK4a, ki-67, HPV