急性淋巴细胞白血病患者ASB2和Jak3基因mRNA表达的研究

doi:10.3760/cma.j.issn.1673422X.2016.07.008

摘要/Abstract

摘要： 目的研究急性淋巴细胞白血病（ALL）患者骨髓中锚蛋白重复序列和抑制细胞因子信号盒蛋白2（ASB2）和Janus激酶3（Jak3）mRNA的表达及其两者的相关性。方法收集初诊的48例ALL患者（37例B细胞ALL，11例T细胞ALL）和34例非白血病患者（对照组）骨髓，采用实时荧光定量PCR检测骨髓中ASB2和Jak3 mRNA表达情况。结果B细胞ALL和T细胞ALL患者骨髓中ASB2 mRNA表达量相对于对照组分别升高了32.7倍和68.5倍，差异均有统计学意义（t=20.1，P＜0.01；t=23.1，P＜0.01)，Jak3 mRNA表达量较对照组分别升高了2 336.3和7 131.5倍（t=70.2，P＜0.01；t=90.4，P＜0.01）。ASB2和Jak3 mRNA表达量具有相关性（r=0.523，P＜0.001）。结论ASB2和Jak3在ALL患者骨髓中异常表达，且具有正相关性，两者可能共同参与白血病细胞的恶性增殖和异常分化。

关键词: 白血病, 淋巴样, 骨髓, , 聚合酶链反应, , 锚蛋白重复序列和抑制细胞因子信号盒蛋白2, , Janus激酶3

Abstract: ObjectiveTo explore the expression of ankyrinrepeat SOCS box containing protein 2 (ASB2) and Janus kinase 3 (Jak3) mRNA in bone marrow plasma mononuclear cells of patients with acute lymphoblastic leukemia (ALL) and discuss the correlation of them. MethodsBone marrow plasma was collected from 48 patients of newly diagnosed ALL (37 cases BALL and 11 cases TALL) and 34 non leukemia patients (control group), respectively. Expression levels of ASB2 and Jak3 mRNA were detected by realtime quantitative PCR. ResultsThe expression of ASB2 mRNA in BALL and TALL were significantly different compared to control group (t=14.2, P＜0.01; t=15.2, P＜0.01), which were 68.5, 32.7 folded more than control group, respectively. Moreover, the expression level of Jak3 mRNA in BALL group and TALL group were 2 336.1 and 7 131.5 folded more than control group (t=37.3, P＜0.01; t=37.6, P＜0.01). At last, the expression of ASB2 and Jak3 gene was positive correlation (r=0.523, P＜0.001). ConclusionASB2 and Jak3 are expressed abnormally in ALL patients and the correlation of them is positive. ASB2 and Jak3 may be related to the proliferation and differentiation in leukemia cells.

Key words: Leukemia, lymphoid, Bone marrow, Polymerase chain reaction;, , Ankyrinrepeat SOCS box containing protein 2, Janus kinase 3

邓亚云, 吴薇, 张平安. 急性淋巴细胞白血病患者ASB2和Jak3基因mRNA表达的研究[J]. 国际肿瘤学杂志, 2016, 43(7): 515-518.

DENG Ya-Yun, WU Wei, ZHANG Ping-An. Investigaion about the mRNA expression of ASB2 and Jak3 in patients with acute lymphoblastic leukemia[J]. Journal of International Oncology, 2016, 43(7): 515-518.

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