国际肿瘤学杂志 ›› 2013, Vol. 40 ›› Issue (12): 936-940.doi: 10.3760/cma.j.issn.1673-422X.2013.12.018

• 论著 • 上一篇    下一篇

微小RNA-126和微小RNA-7在食管鳞状细胞 癌中的表达及临床意义

王江峰, 凌志强, 毛伟敏   

  1. 310022 杭州,浙江省肿瘤研究所
  • 出版日期:2013-12-10 发布日期:2013-12-26
  • 通讯作者: 毛伟敏 E-mail:maowm@163.com
  • 基金资助:

    卫生部科研基金(WKJ20102004);浙江省重大科技专项计划项目(2011C130391)

Expressions and clinical significances of microRNA-126 and microRNA-7 in esophageal squamous cell carcinoma

WANG  Jiang-Feng, LING  Zhi-Qiang, MAO  Wei-Min   

  1. Zhejiang Cancer Research Institute, Hangzhou 310022, China
  • Online:2013-12-10 Published:2013-12-26
  • Contact: MAO Wei-Min E-mail:maowm@163.com

摘要: 目的探讨微小RNA126(miR126)和微小RNA7(miR7)在食管鳞状细胞癌(ESCC)中的表达情况及其与ESCC临床病理特征、预后之间的关系。方法采用实时荧光定量聚合酶链反应法检测116例ESCC组织和癌旁正常组织中miR126和miR7的表达差异,并对miR126和miR7表达情况与ESCC患者临床病理特征、预后的相关性进行统计学分析。结果miR126不同程度低表达病例数为73(62.9%),正常表达病例数为35(30.2%),高表达病例数为8(6.9%);miR7不同程度低表达病例数为52(44.8%),正常表达病例数为35(30.2%),高表达病例数为29(25.0%)。miR126和miR7表达降低组无瘤生存期均较非降低组短(χ2=4.268,P<0.05;χ2=4.993,P<0.05);miR126低表达与ESCC肿瘤位置、家族史和饮酒相关(χ2=14.564, P<0.05;χ2=5.691,P<0.05;χ2=4.971,P<0.05),与性别、年龄、分化程度、浸润程度、淋巴转移、吸烟不相关(均P>0.05);miR7低表达与ESCC的临床病理特征均不相关(均P>0.05)。结论miR126和miR7的低表达可能与ESCC患者预后相关,具有一定的临床检测意义。

关键词: 食管肿瘤, 预后, 微小RNA-126, 微小RNA-7

Abstract: ObjectiveTo detect the expressions of microRNA126 (miR126) and microRNA7 (miR7) in esophageal squamous cell carcinoma (ESCC) and to analyze their correlations with clinicopathologic features and prognosis of ESCC. MethodsThe expressions of miR126 and miR7 in 116 ESCC samples and matched normal tissue samples were detected by realtime PCR. Statistical analysis was used to find the relationships among the expressions of miR126 and miR7, pathological characteristics and prognosis. ResultsLow expression, normal expression and high expression of miR126 were found in 73 (62.9%), 35 (30.2%) and 8 (6.9%) carcinoma samples respectively. Low expression, normal expression and high expression of miR7 were found in 52 (44.8%), 35 (30.2%) and 29 (25.0%) carcinoma samples respectively. The diseasefree survival in patients with low expression of miR126 and miR7 was shorter than that in patients with nonlow expression (χ2=4.268, P<0.05; χ2=4.993, P<0.05). The low expression of miR126 was correlated with tumor location, family history and drinking (χ2=14.564, P<0.05; χ2=5.691, P<0.05; χ2=4.971, P<0.05), but was uncorrelated with gender, age, differentiation, infiltration, lymphatic metastasis and smoking (all P>0.05). The low expression of miR7 was uncorrelated with pathological characteristics of ESCC (all P>0.05). Conclusion The low expressions of miR126 and miR7 may be related to the prognosis of patients with ESCC, and have a certain clinical detection significance.

Key words: Esophageal neoplasms, Prognosis, MicroRNA-126, MicroRNA-7