国际肿瘤学杂志

国际肿瘤学杂志 ›› 2018, Vol. 45 ›› Issue (11): 670-674.doi: 10.3760/cma.j.issn.1673-422X.2018.11.007

• 论著 • 上一篇    下一篇

基于生物信息学数据库分析KIF20A在肝细胞癌中的表达及临床预后意义

李岩，王伟   

  1. 230001 合肥，中国科技大学附属第一医院（安徽省立医院）肿瘤化疗科
  • 出版日期:2018-11-08 发布日期:2018-12-21
  • 通讯作者: 王伟，Email: whouwei@gmail.com E-mail:whouwei@gmail.com
  • 基金资助:
    国家自然科学基金（81201906）；安徽省自然科学基金（1708085QH177）

Expression of KIF20A in hepatocellular carcinoma and its prognostic significance analyzed in bioinformatics database

Li Yan, Wang Wei   

  1. Department of Chemotherapy, First Affiliated Hospital of University of Science and Technology of China (Anhui Provincial Hospital), Hefei 230001, China
  • Online:2018-11-08 Published:2018-12-21
  • Contact: Wang Wei, Email: whouwei@gmail.com E-mail:whouwei@gmail.com
  • Supported by:
    National Natural Science Foundation of China (81201906); Natural Science Foundation of Anhui Province of China (1708085QH177)

PDF

883

摘要： 目的 探索驱动蛋白超家族20A（KIF20A）在肝细胞癌中的表达及临床预后意义。方法 利用GEPIA（Gene Expression Profiling Interactive Analysis）、Oncomine及THPA（The Human Protein Atlas）生物信息学在线分析网站，挖掘分析大型癌症公共数据癌症基因组图谱（TCGA）及GEO（Gene Expression Omnibus）中肝癌KIF20A mRNA及蛋白的表达信息，基于TCGA中的肝癌数据采用Kaplan-Meier法进行生存分析，log-rank法进行生存率的比较，并对KIF20A和磷脂酰肌醇3-激酶（PI3K）/蛋白激酶B（AKT）信号通路中部分关键分子的表达进行Pearson相关性分析。结果GEPIA中检索到369例肝癌及50例正常肝组织中包含KIF20A mRNA的表达含量信息，Oncomine中检索到4项有关KIF20A mRNA在肝癌组织中表达差异的研究。结果 均发现，与正常肝组织相比，KIF20A mRNA表达水平在肝癌中显著升高（P<0.001；t=8.766，P＜0.001；t=24.329，P＜0.001；t=7.398，P＜0.001；t=3.191，P=0.001）。THPA在线网站分析表明，KIF20A蛋白在正常肝组织中呈低表达或不表达，而在肝癌组织中呈现明显高表达。这一结果与mRNA分析结果相一致。生存分析发现，KIF20A表达量与肝癌患者总生存期和无瘤生存期相关，KIF20A表达高的患者预后较差（P=0.003；P<0.001）。进一步相关分析发现，肝癌中KIF20A基因表达与磷脂酰肌醇3-激酶催化亚基α（PIK3CA）、AKT1、哺乳动物雷帕霉素靶蛋白（mTOR）、缺氧诱导因子1α（HIF1A）及血管内皮细胞生长因子A（VEGFA）基因均呈正相关（R=0.43，P＜0.001；R=0.29，P＜0.001；R=0.18，P＜0.001；R=0.39，P＜0.001；R=0.37，P＜0.001）。结论 利用生物信息学的方法分析发现，KIF20A在肝癌组织中高表达，且与肝癌患者预后相关，其机制可能与调控PI3K/AKT信号通路有关，值得未来进一步深入研究。

关键词: 肝肿瘤, 驱动蛋白, 预后, 生物信息学

Abstract: Objective To explore the expression of kinesin family member 20A (KIF20A) in hepatocellular carcinoma (HCC) and its prognostic significance. Methods By using bioinformatics methods in Gene Expression Profiling Interactive Analysis (GEPIA), Oncomine and The Human Protein Atlas (THPA) online analysis websites, the mRNA and protein expression information of KIF20A in HCC was analyzed based on the large cancer public data including The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). The Kaplan-Meier method was used to perform patients′ survival analysis based on TCGA liver cancer data, and the survival rates were compared by log-rank method. Pearson correlation analysis was performed to investigate the expression of KIF20A and some key molecules in phosphoinositide 3-kinase (PI3K)/protein kinase B (AKT) signaling pathways. Results GEPIA retrieved 369 cases of HCC and 50 cases of normal liver tissue containing KIF20A mRNA expression information. Oncomine retrieved a total of 4 studies on KIF20A mRNA in HCC. All results showed that compared with the normal liver tissues, the mRNA expression level of KIF20A was significantly higher in HCC tissues (P<0.001; t=8.766, P＜0.001; t=24.329, P＜0.001; t=7.398, P＜0.001; t=3.191, P=0.001). Besides, THPA online analysis websites showed that KIF20A protein was low or not expressed in normal liver tissues, but it was significantly higher in HCC tissues, and this result was consistent with the mRNA analysis result. Moreover, the survival analysis found that the expression of KIF20A was correlated with the overall survival (OS) and disease-free survival (DFS) of HCC patients, and the prognosis of patients with high KIF20A expression was poor (P=0.003; P<0.001). Additionally, further correlation analysis found that KIF20A gene was positively correlated with phosphatidylinositol 3-kinase catalytic subunit alpha (PIK3CA), AKT1, mammalian target of rapamycin (mTOR), hypoxia-inducible factor 1α (HIF1A) and vascular endothelial growth factor A (VEGFA) genes in HCC (R=0.43, P＜0.001; R=0.29, P＜0.001; R=0.18, P＜0.001; R=0.39, P＜0.001; R=0.37, P＜0.001). Conclusion Bioinformatics analysis results indicate that KIF20A is highly expressed in HCC tissues and KIF20A is associated with the prognosis of HCC patients, the mechanism of which may be related to the regulation of PI3K/AKT signaling pathway. It is worth further study in the future.

Key words: Liver neoplasms, Kinesin, Prognosis, Bioinformatics