HOXB1与miR-3175在人胶质瘤中的表达及临床意义

doi:10.3760/cma.j.issn.1673-422X.2016.11.004

国际肿瘤学杂志 ›› 2016, Vol. 43 ›› Issue (11): 817-821.doi: 10.3760/cma.j.issn.1673-422X.2016.11.004

HOXB1与miR-3175在人胶质瘤中的表达及临床意义

周海霞，韩亮，祝子峰，魏君，田宇，李朝晖

130033 长春，吉林大学中日联谊医院特需病房（周海霞），病理科（韩亮），神经外科（魏君、田宇、李朝晖）；吉林大学第一医院介入治疗科（祝子峰）

收稿日期:2016-02-21 出版日期:2016-11-08 发布日期:2016-11-02
通讯作者: 李朝晖，Email: ruosong@163.com E-mail:ruosong@163.com
基金资助:
吉林省科技发展计划国际科技合作项目（20130413028GH）；吉林省卫生计生青年科研基金（2015Q005）

The expressions and clinical significances of HOXB1 and miR-3175 in human glioma

Zhou Haixia, Han Liang, Zhu Zifeng, Wei Jun, Tian Yu, Li Zhaohui

Special Ward, ChinaJapan Union Hospital of Jilin University, Changchun 130033, China

Received:2016-02-21 Online:2016-11-08 Published:2016-11-02
Contact: Li Zhaohui E-mail:ruosong@163.com
Supported by:
Jilin Provincial Science and Technology Development Plan for International Cooperation Program (20130413028GH); Youth Scientific Research Fund from Health and Family Planning Commission of Jilin Province (2015Q005)

摘要/Abstract

摘要： 目的探讨同源异型盒基因B1（HOXB1）与微小RNA3175(miR3175)在人胶质瘤中表达的关系及临床意义。方法通过实时荧光定量PCR（qRTPCR）检测60例人胶质瘤组织和15例正常脑组织中HOXB1与miR3175的表达，采用Spearman相关分析法分析人胶质瘤组织中HOXB1、miR3175表达的相关性，分析HOXB1、miR3175表达与临床病理特征的关系，采用KaplanMeier评估HOXB1、miR3175与患者生存期的关系，采用COX回归模型分析患者预后因素。结果较正常脑组织，HOXB1在人胶质瘤组织中低表达（1.498±0.323∶0.946±0.588，t=-5.680，P=0.000），miR3175在人胶质瘤组织中高表达（1.008±0.355∶2.076±0.841，t=4.274，P=0.000），并且在人胶质瘤组织中HOXB1与miR3175表达呈负相关（r=-0.601，P=0.000）。HOXB1表达与肿瘤分级相关（χ2=4.848，P=0.028），miR3175表达与肿瘤分级（χ2=5.640，P=0.018）、Karnofsky功能状态评分（χ2=4.785，P=0.029）相关。KaplanMeier分析结果显示HOXB1、miR3175高表达组与低表达组中位生存期［HOXB1:（21.0±4.0）个月∶(7.0±0.8)个月；miR3175: （6.0±0.6）个月∶(16.0±5.8)个月］差异有统计学意义（χ2=7.495，P=0.006；χ2=9.591，P=0.002）。COX回归模型分析结果显示肿瘤分级（RR=6.556, 95%CI为1.196～35.952，P=0.002）、HOXB1（RR=0.018，95%CI为0.001～0.312，P=0.006）和miR3175（RR=2.098，95%CI为1.663～7.513，P=0.037）是影响胶质瘤患者预后的独立因素。结论HOXB1与miR3175在人胶质瘤中的表达呈负相关，并与胶质瘤组织的恶性程度、胶质瘤患者的生存期及预后密切相关。

关键词: 神经胶质瘤, 预后, 微RNAs, 同源异型盒基因B1

Abstract: ObjectiveTo explore the relationship and the clinical significance of homeobox gene B1 (HOXB1) and microRNA3175 (miR3175) expressions in human glioma. MethodsThe expression levels of HOXB1 and miR3175 in 60 glioma tissues and 15 normal brain tissues were analyzed by realtime fluorescent quantitative PCR (qRTPCR). Spearman rank correlation analysis was performed to explore the relationship between HOXB1 and miR3175 in human glioma tissues. The relationship between HOXB1 (or miR3175) and clinical pathological characteristics of glioma patients was analyzed. The correlation of HOXB1 (or miR3175) and survival rate was calculated by KaplanMeier. And COX regression models were used to assess the prognostic factors. ResultsCompared with normal brain tissues, the expression of HOXB1 was significantly decreased in glioma tissues (1.498±0.323 vs. 0.946±0.588, t=-5.680, P=0.000)； and the expression of miR3175 was obviously increased in glioma tissues (1.008±0.355 vs. 2.076±0.841, t=4.274, P=0.000), and HOXB1 expression was negatively correlated with miR3175 expression in glioma tissues (r=-0.601, P=0.000). HOXB1 expression was related with histologic grade (χ2=4.848, P=0.028), and miR3175 expression was related with histologic grade (χ2=5.640, P=0.018) and Karnofsky score (χ2=4.785, P=0.029）. The results of KaplanMeier revealed that there were significant differences in median survival time between HOXB1 (or miR3175) highexpression group and HOXB1 (or miR3175) lowexpression group ［HOXB1: (21.0±4.0)months vs. (7.0±0.8) months; χ2=7.495, P=0.006; miR3175: (6.0±0.6) months vs. (16.0±5.8) months; χ2=9.591, P=0.002］. COX regression models showed that tumor degree (RR=6.556, 95%CI: 1.19635.952, P=0.002), HOXB1 (RR=0.018, 95%CI: 0.0010.312, P=0.006) and miR3175 (RR=2.098, 95%CI: 1.6637.513, P=0.037) were independent prognostic factors for prognosis. ConclusionThe HOXB1 expression may be negatively correlated with miR3175 in human glioma tissues, and the expression levels of HOXB1 and miR3175 are associated with the glioma malignant degree, survival time and prognosis of glioma patients.

Key words: Glioma, Prognosis, MicroRNAs, Homeobox gene B1

周海霞，韩亮，祝子峰，魏君，田宇，李朝晖. HOXB1与miR-3175在人胶质瘤中的表达及临床意义[J]. 国际肿瘤学杂志, 2016, 43(11): 817-821.

Zhou Haixia, Han Liang, Zhu Zifeng, Wei Jun, Tian Yu, Li Zhaohui. The expressions and clinical significances of HOXB1 and miR-3175 in human glioma[J]. Journal of International Oncology, 2016, 43(11): 817-821.

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HOXB1与miR-3175在人胶质瘤中的表达及临床意义

The expressions and clinical significances of HOXB1 and miR-3175 in human glioma

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