国际肿瘤学杂志

国际肿瘤学杂志 ›› 2018, Vol. 45 ›› Issue (3): 139-142.doi: 10.3760/cma.j.issn.1673-422X.2018.03.003

• 论著 • 上一篇    下一篇

miR-138靶向作用Sema4C对脑胶质瘤细胞侵袭转移的影响

李会兵，姚娟   

  1. 432300 湖北省孝感市汉川市人民医院神经外科（李会兵），静脉用药调配中心（姚娟）
  • 收稿日期:2017-11-28 出版日期:2018-03-08 发布日期:2018-05-16
  • 通讯作者: 姚娟 E-mail:姚娟，Email: 37403542@qq.com

Effects of miR-138 on invasion of brain glioma cells through targeting Sema4C

Li Huibing, Yao Juan   

  1. Department of Neurosurgy, Hanchuan City People′s Hospital, Xiaogan City of Hubei Province, Xiaogan 432300, China
  • Received:2017-11-28 Online:2018-03-08 Published:2018-05-16
  • Contact: Yao Juan E-mail:姚娟，Email: 37403542@qq.com

PDF

766

摘要： 目的研究微小RNA-138（miR-138）对脑胶质瘤细胞侵袭转移的影响及其机制。方法实时荧光定量聚合酶链反应（qRT-PCR）检测miR138在60例脑胶质瘤组织及其癌旁组织中的表达，分析miR-138表达与脑胶质瘤分级的关系；体外对脑胶质瘤细胞株U251和U87分别转染miR-138类似物（miR-138 mimic）及阴性对照（miRNC）；qRTPCR检测miR-138表达情况；Transwell实验检测U251和U87细胞侵袭转移能力；Western blotting检测miR138靶向蛋白轴突导向蛋白4C（Sema4C）的表达。结果脑胶质瘤组织中miR-138表达水平（2.46±1.07）较癌旁正常脑组织（4.83±1.16）明显降低（t=-11.631，P＜0.001），且miR-138表达与肿瘤恶性程度呈负相关（r=-0.563，P=0.001）。转染miR-138 mimic组细胞miR-138表达（U251：3.96±0.16；U87：4.43±0.96）明显高于转染miRNC组（U251：2.32±0.36；U87：2.58±0.62）（t=7.253，P＜0.001；t=8.872，P＜0.001）。miR-138 mimic组（U251：89±9；U87：95±10）细胞侵袭转移能力明显低于转染miR-NC组（U251：206±15；U87：240±20）（t=36.629，P＜0.001；t=35.521，P＜0.001）。miR-138 mimic组细胞Sema4C表达（U251：0.41±0.06；U87：0.36±0.03）明显低于miRNC组（U251：1.01±0.08；U87：1.03±0.13）（t=-32.862，P＜0.001；t=-27.512，P＜0.001）。结论上调miR138表达可抑制脑胶质瘤细胞侵袭转移，可能与负向调节Sema4C表达有关。

关键词: 神经胶质瘤, 肿瘤浸润, 肿瘤转移, 微小RNA-138, 轴突导向蛋白4C

Abstract: ObjectiveTo explore the effects and mechanism of microRNA-138 (miR-138) on brain glioma cells invasion and migration. MethodsThe expression of miR138 was detected by real time quantitative polymerase chain reaction (qRT-PCR) in 60 cases of glioma tissues and paracarcinoma tissues, and the relationship between miR138 expression and glioma grading was analyzed. Human glioma cells line U87 and U251 were transfected with miR138 mimic and negative control (miR-NC). The expression of miR138 was detected by qRTPCR. The migration and invasion abilities were tested by Transwell assay, and the expression of semaphoring 4C (Sema4C) protein was tested by Western blotting. ResultsThe expression level of miR-138 in glioma tissues (2.46±1.07) was significantly lower than that in normal brain tissues (4.83±1.16, t=-11.631, P＜0.001), and miR138 expression was negatively correlated with tumor grade (r=-0.563, P=0.001). The expression level of miR138 in cells was significantly higher after being transfected with miR-138 mimic (U251: 3.96±0.16; U87: 4.43±0.96) than miR-NC (U251: 2.32±0.36; U87: 2.58±0.62, t=7.253, P＜0.001; t=8.872, P＜0.001). The ability of invasion and migration were lower after being transfected with miR-138 mimic (U251: 89±9; U87: 95±10) than miR-NC (U251: 206±15; U87: 240±20, t=36.629, P＜0.001; t=35.521, P＜0.001). The expression of Sema4C protein was lower after being transfected with miR-138 mimic (U251: 0.41±0.06; U87: 0.36±0.03) than miRNC (U251: 1.01±0.08; U87: 1.03±0.13, t=-32.862, P＜0.001; t=-27.512, P＜0.001). ConclusionThe upregulated expression of miR138 can suppress glioma cells migration and invasion, which might be related to the negative regulation expression of Sema4C protein.

Key words: Glioma, Neoplasm invasiveness, Neoplasm metastasis, microRNA-138, Semaphoring 4C