国际肿瘤学杂志

国际肿瘤学杂志 ›› 2015, Vol. 42 ›› Issue (7): 492-495.doi: 10.3760/cma.j.issn.1673-422X.2015.07.004

• 论著 • 上一篇    下一篇

Numb基因负性调节CXCR4表达抑制膀胱癌细胞BIU-87的增殖和迁移

司马晋,张保,王保军,于源滋,艾青,张旭   

  1. 100049 北京，航天中心医院（北京大学航天临床医学院）泌尿外科（司马晋、张保）；解放军总医院泌尿外科（王保军、艾青、张旭）；山东省立医院消化科（于源滋）
  • 出版日期:2015-07-08 发布日期:2015-05-26
  • 通讯作者: 张保 E-mail: baoztj@sina.com
  • 基金资助:

    国家自然科学基金(81100561)；航天科工集团医疗卫生科研项目(2014LCYL007)

Numb inhibits proliferation and migration of human bladder cancer cell line BIU87 by negative modulating CXCR4

Sima Jin, Zhang Bao, Wang Baojun, Yu Yuanzi, Ai Qing, Zhang Xu   

  1. Department of Urology, Aerospace Center Hospital, Aerospace Clinical Medical School of Peking University, Beijing 100049, China
  • Online:2015-07-08 Published:2015-05-26
  • Contact: Zhang Bao E-mail: baoztj@sina.com

PDF

1240

被引次数

1

摘要： 目的研究Numb基因对趋化因子受体4（CXCR4）表达的影响以及对膀胱癌细胞增殖和迁移的影响。方法实验分为3组：实验组（在膀胱癌细胞BIU87中转染NumbORF表达质粒）、阴性对照组（转染空载体）和空白对照组（未行转染处理）。使用荧光定量PCR和Western blotting检测各组细胞Numb和CXCR4的表达。使用MTS法和Transwell小室法检测细胞增殖和迁移能力。结果实验组、阴性对照组和空白对照组中Numb基因表达水平分别为31.044±3.350、4.401±0.567和4.287±0.341，差异有统计学意义（F=183.418, P=0.000）；实验组、阴性对照组和空白对照组的CXCR4表达水平分别为0.344±0.167、0.996±0.148和1.010±0.106，差异有统计学意义（F=21.355, P=0.002）。细胞增殖检测中实验组、阴性对照组和空白对照组的48 h吸光度值分别为0.615±0.057、0.987±0.063和0.957±0.066，差异有统计学意义(F=33.210, P=0.001）。迁移实验中，实验组、阴性对照组和空白对照组的穿膜细胞数分别为（164.667±19.858）个、（670.133±38.760）个和（667.533±27.610）个，差异有统计学意义（F=286.788, P=0.000）。 结论Numb可能通过负性调节CXCR4表达抑制膀胱癌细胞的增殖和迁移。

关键词: 膀胱肿瘤, 受体, CXCR4, 细胞增殖, 细胞迁移测定

Abstract: ObjectiveTo study the effect of Numb gene on CXCR4 expression and proliferation and migration of bladder cancer. MethodsHuman bladder cancer cells were divided into 3 groups: experimental group (transfected with NumbORF plasmid), negativecontrol group (transfected with blank vector), and blankcontrol group (no DNA transfected in). The expressions of Numb and CXCR4 were detected by realtime PCR and Western blotting respectively. Cell proliferation and migration were measured by MTS and Transwell assay respectively. ResultsNumb expressions in experimental group, negativecontrol group and blankcontrol group were 31.044±3.350, 4.401±0.567 and 4.287±0.341 respectively, with a statistical significance (F=183.418, P=0.000). CXCR4 levels in experimental group, negativecontrol group and blankcontrol group were 0.344±0.167, 0.996±0.148 and 1.010±0.106 respectively, with a statistical significance (F=21.355, P=0.002). In MTS assay, the absorb values in experimental group, negativecontrol group and blankcontrol group were 0.615±0.057, 0.987±0.063 and 0.957±0.066, with a statistical difference (F=33.210, P=0.001). In Transwell assay, the numbers of migratory cells in experimental group, negativecontrol group and blankcontrol group were 164.667±19.858, 670.133±38.760 and 667.533±27.610, with a statistical significance (F=286.788, P=0.000). ConclusionOverexpression of Numb can suppress the ability of proliferation and migration of the BIU87 cells through downregulation of CXCR4 expression in human bladder cancer.

Key words: Urinary bladder neoplasms, Receptors, CXCR4, Cell proliferation, Cell migration assays