国际肿瘤学杂志

国际肿瘤学杂志 ›› 2013, Vol. 40 ›› Issue (8): 715-720.

• 论著 • 上一篇    

EphA8在结直肠癌组织中的表达和临床意义

鲁信军, 李霄   

  1.  725000安康市中医院普通外科（鲁信军）；第四军医大学西京医院肝胆胰脾外科（李霄）

  • 出版日期:2013-09-08 发布日期:2013-09-16
  • 通讯作者: 李霄，E-mail: lixiao0076@163.com E-mail:lixiao0076@163.com

Expression and clinical significance of EphA8 in colon cancer

LU  Xin-Jun, LI  Xiao   

  1. Department of General Surgery, Ankang Hospital of Traditional Chinese Medicine, Ankang 725000, China
  • Online:2013-09-08 Published:2013-09-16
  • Contact: LI Xiao，E-mail: lixiao0076@163.com E-mail:lixiao0076@163.com

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摘要： 目的 探讨EphA8在结肠癌组织中的表达情况和临床意义。方法 采用实时定量RT-PCR和Western blot检测EphA8 mRNA和蛋白在正常结肠黏膜细胞株和结肠癌细胞株中的表达情况。采用免疫组织化学法检测98例结肠癌组织和12例正常结肠组织中EphA8和血管内皮生长因子（VEGF）的蛋白表达情况，同时使用CD31染色检测肿瘤微血管密度（MVD）情况，并分析EphA8与VEGF和MVD的关系及其与病理分期、预后的关系。结果 EphA8的mRNA和蛋白在结肠癌细胞株的表达明显高于正常结肠黏膜细胞株（t=11.98, 13.54, P<0.001; t=4.63, P=0.006; t=4.92, P=0.004）。免疫组织化学结果表明EphA8蛋白在结肠癌组织高表达，且癌组织中EphA8的蛋白表达与肿瘤大小（χ2=22.97, P<0.001）、分期（P<0.001）、分化程度（P=0.007）、淋巴结转移（χ2=30.15, P<0.001）、远处转移（χ2=6.97, P=0.008）以及患者生存率（χ2=17.3, P<0.001）存在显著相关性，而与患者的性别和年龄没有明显相关性（χ2=1.36, P=0.30; χ2=0.83, P=0.44）。此外，EphA8与VEGF和MVD的表达呈正相关（r=0.434，P<0.001; r=0.584，P<0.001）。结论 EphA8在结肠癌的恶性发展和转移中有重要作用，因此，EphA8对于结肠癌的治疗干预和预后判断具有潜在的临床应用价值。

关键词: 结肠肿瘤, 受体, EphA8, 血管生成素类

Abstract: Objective To investigate the expression of EphA8 in colon cancer and its clinical significance. Methods Quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot were respectively used to assess the expressions of EphA8 mRNA and protein in normal colon mucosa cell line and colon cancer cell lines. Immunohistochemistry for EphA8 and vascular endothelial growth factor (VEGF) was performed in 98 cases of colon cancer and 12 matched normal mucosa tissues. CD31 immunohistochemical staining was used for microvascular density (MVD) counting. The relationships between the expression of EphA8 and the expression of VEGF and MVD, the clinical pathological significance, and the prognosis of patients were analyzed by statistical methods. Results EphA8 mRNA and protein expression was significantly higher in colon cancer cell lines than that in normal mucosa cell lines (t=11.98, 13.54, P<0.001; t=4.63, P=0.006; t=4.92, P=0.004). The high expression of EphA8 was closely related to tumor size (χ2=22.97, P<0.001), TNM stage (P<0.001), differentiated degree (P=0.007) and lymph node metastasis (χ2=30.15, P<0.001), distant metastasis (χ2=6.97, P=0.008) and poor survival rate (χ2=17.3, P<0.001). But it was not associated with the gender and age (χ2=1.36, P=0.30; χ2=0.83, P=0.44). Besides, EphA8 had positive correlation with the VEGF and MVD (r=0.434, P<0.001; r=0.584, P<0.001). Conclusions EphA8 may play important roles in the development and metastasis of colon cancer, which has potential clinical values in the therapeutic intervention and prognosis of colon cancer.

Key words: Colonic neoplasms, Receptor, EphA8, Angiopoietins