国际肿瘤学杂志

国际肿瘤学杂志 ›› 2016, Vol. 43 ›› Issue (8): 655-658.doi: 10.3760/cma.j.issn.1673422X.2016.09.004

• 论著 • 上一篇    下一篇

MET基因在非小细胞肺癌中的表达及其意义

王波, 王辉, 齐康, 张连斌   

  1. 100853北京，中国人民解放军总医院胸外科
  • 出版日期:2016-09-08 发布日期:2016-08-04
  • 通讯作者: 张连斌，Email: doctorzhang301@126.com E-mail:doctorzhang301@126.com
  • 基金资助:

    解放军总医院临床科研扶持基金（2012FCTSYS4015）

Expression and significance of MET gene in nonsmall cell lung cancer

Wang Bo, Wang Hui, Qi Kang, Zhang Lianbin   

  1. Department of Thoracic Surgery, Chinese People′s Liberation Army General Hospital, Beijing 100853, China
  • Online:2016-09-08 Published:2016-08-04
  • Contact: Zhang Lianbin E-mail:doctorzhang301@126.com
  • Supported by:

    Clinical Research Fund from the General Hospital of People′s Liberation Army (2012FCTSYS4015)

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摘要： 【摘要】目的探讨间质上皮转化因子（MET）基因mRNA在非小细胞肺癌（NSCLC）中的表达及其与临床病理特征的关系。方法选取2011年6月—2013年11月在中国人民解放军总医院病理确诊为NSCLC的48例患者，所有患者均未接受术前治疗。采用分支DNA液相芯片技术对获取的肿瘤组织中MET基因mRNA表达水平进行检测，分析MET基因mRNA表达水平与临床病理特征的关系。结果MET基因mRNA以中度表达为主，低度、中度和高度表达的比例分别为22.9%、50.0%和27.1%。MET基因mRNA表达与患者病理类型（χ2=7.183，P=0.020）和TNM分期（χ2=24.566，P=0.017）有关，与性别（χ2=0.566，P=0.754）、年龄（χ2=1.857，P=0.395）、吸烟史（χ2=4.959，P=0.084）、分化程度（χ2=5.749，P=0.067）、淋巴结转移（χ2=1.631，P=0.442）和远处转移（χ2=4.261，P=0.119）均无关。结论MET基因mRNA在NSCLC中更易呈现中、高度表达，而且MET基因可以作为判断肿瘤病理类型的生物学标志物。

关键词: 癌, 非小细胞肺, RNA, 信使, 间质上皮转化因子

Abstract: 【Abstract】ObjectiveTo detect the mRNA expression of mesenchymalepithelial transition factor (MET) gene in patients with nonsmall cell lung cancer (NSCLC), and to investigate the relationship and clinical significance between the mRNA expression of MET gene and clinical pathological characteristics. MethodsFrom June 2011 to November 2013, 48 patients with pathologically confirmed NSCLC in Chinese People′s Liberation Army General Hospital were selected. All patients included in the study were not treated before surgery. The branched DNA liquid chip technology was used to detect mRNA expression of MET gene in tumor tissues. The relationship between mRNA expression of MET gene and clinical pathological characteristics was analyzed. ResultsThe constituent ratios of low, moderate and high mRNA expression level of MET gene were 22.9%, 50.0% and 27.1% respectively, mainly for the moderate expression. The mRNA expression of MET gene was related to the pathologic type (χ2=7.183, P=0.020) and TNM stage (χ2=24.566, P=0.017) of the patients; but it was not related to the gender (χ2=0.566, P=0.754), age (χ2=1.857, P=0.395), smoking history (χ2=4.959, P=0.084), degrees of differentiation (χ2=5.749, P=0.067), lymph node metastasis (χ2=1.631, P=0.442) and distant metastasis (χ2=4.261, P=0.119). ConclusionmRNA expression of MET gene is more likely to present moderate and higher level in NSCLC patients. MET gene can also be used as a biomarker for judging tumor pathological type.

Key words: Carcinoma, nonsmallcell lung, RNA, messenger, Mesenchymalepithelial transition factor