非小细胞肺癌TUBB3基因表达调控的生物信息学分析

doi:10.3760/cma.j.issn.1673-422X.2020.03.004

摘要/Abstract

摘要：

目的应用生物信息学的方法研究β-微管蛋白亚型TUBB3在非小细胞肺癌(NSCLC)中的表达及其分子调控机制。方法通过GEO数据集(GEO profile)、转录因子和miRNA调控关系数据库(TransmiR)、综合型的miRNA靶基因数据库(miRWalk)、转录因子结合位点搜索(ConSite)等,研究TUBB3上游转录因子、共表达基因及与其有相互作用的miRNA等,阐明NSCLC中TUBB3表达的分子调控机制及其可能的作用。结果 TUBB3的启动子存在Snail、n-MYC等多个与NSCLC相关的转录因子结合位点。其在转录后水平受到miR-200家族、miR-342-3p、miR-410等miRNA的调控,这些miRNA与NSCLC的增殖与侵袭有关。TUBB3与HDGF、GMPS、MRPL9、PMAIP1和SLBP有共表达行为,受转录因子Snail共同调控。其中SLBP、PMAIP1及转录因子Snail与细胞周期和细胞凋亡存在一定的联系。结论 NSCLC中TUBB3的表达受到多个层面的调控,其可能参与了细胞周期和凋亡,同时与NSCLC细胞增殖与侵袭存在一定的联系。

关键词: 癌, 非小细胞肺, TUBB3, 基因调控

Abstract:

Objective To predict the expression and molecular regulatory mechanisms of βⅢ-tubulin (TUBB3) in non-small cell lung cancer (NSCLC) using bioinformatics methods. Methods The GEO profile, TransmiR, miRWalk and ConSite database were employed in the present study. The upstream transcription factor, co-expression gene and the interactive miRNA of TUBB3 were studied to determine the molecular regulatory mechanisms and possible role of TUBB3 in NSCLC. Results The promoter region of TUBB3 could be recognized by transcription factors associated with NSCLC including Snail and n-MYC and so on. At the post-transcription level, the TUBB3 could be regulated by miRNAs including miR-200 family, miR-342-3p, miR-410 and so on. These miRNAs were associated with proliferation and invasion of NSCLC. TUBB3 was co-expressed with HDGF, GMPS, MRPL9, PMAIP1 and SLBP, and they were co-regulated by the transcription factor Snail. SLBP, PMAIP1 and transcription factor Snail were related to cell cycle and apoptosis. Conclusion The expression of TUBB3 can be regulated at multiple levels in NSCLC. It may take part in cell cycle and apoptosis regulation in NSCLC, and further influence proliferation and invasion of cancer cells.

Key words: Carcinoma, non-small-cell lung, TUBB3, Gene regulation

赵云龙, 李少军, 李璟波, 陈平, 刘阳. 非小细胞肺癌TUBB3基因表达调控的生物信息学分析[J]. 国际肿瘤学杂志, 2020, 47(3): 146-150.

Zhao Yunlong, Li Shaojun, Li Jingbo, Chen Ping, Liu Yang. Bioinformatics analysis of TUBB3 expression and its regulatory mechanisms in non-small cell lung cancer[J]. Journal of International Oncology, 2020, 47(3): 146-150.

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