国际肿瘤学杂志

国际肿瘤学杂志 ›› 2014, Vol. 41 ›› Issue (2): 147-149.doi: 10.3760/cma.j.issn.1673-422X.2014.02.020

• 论著 • 上一篇    下一篇

IL-6和IL-8在正常胃组织、胃溃疡及胃癌组织中的表达

赵志威, 朴大勋, 姜涛, 张哲男, 王剑冰, 荆琼优   

  1. 150001 哈尔滨医科大学附属第一医院普外科
  • 收稿日期:2013-08-19 修回日期:2013-10-20 出版日期:2014-02-08 发布日期:2014-01-26
  • 通讯作者: 朴大勋，E-mail: piaodaxun@sina.com E-mail:piaodaxun@sina.com
  • 基金资助:

    黑龙江省科学技术计划项目 (LC02C15)

Expressions of IL-6 and IL-8 in normal gastric tissue, gastric ulcer and gastric cancer

Zhao Zhiwei, Piao Daxun, Jiang Tao, Zhang Zhenan, Wang Jianbing, Jing Qiongyou   

  1. Department of General Surgery, First Affiliated Hospital of Harbin Medical University, Harbin 150001, China
  • Received:2013-08-19 Revised:2013-10-20 Online:2014-02-08 Published:2014-01-26
  • Contact: Piao Daxun E-mail:piaodaxun@sina.com

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1348

被引次数

9

摘要： 目的 探讨白细胞介素（IL）-6和IL-8在正常胃组织、胃溃疡及胃癌组织中的表达情况。方法 采用酶联免疫吸附法（ELISA）测定正常对照组（33例）、胃溃疡患者（30例）和准备手术的胃癌患者（52例）血浆中IL-6和IL-8的表达水平，并随访胃癌患者术后1周的IL-6、IL-8表达水平；免疫组织化学法检测胃癌周围正常组织（45例）、胃溃疡组织（35例）和胃癌组织（45例）标本中IL-6和IL-8的表达。结果 胃癌患者（术前和术后）血浆中IL-6、IL-8的表达明显高于胃溃疡和正常对照组（均P＜0.01），胃癌患者术后IL-6和IL-8水平较术前明显降低(P＜0.01)。癌周正常组织、胃溃疡和胃癌组织中IL-6和IL-8蛋白阳性表达率依次上升，且差异有统计学意义（χ2 =38.87，P＜0.01；χ2 =42.23，P＜0.01）。 结论 IL-6和IL-8在胃癌患者血浆和胃癌组织中均表达上调，检测患者血浆和病理组织中的IL-6、IL-8水平有助于判断病情和评估预后。

关键词: 白细胞介素6, 白细胞介素8, 胃肿瘤, 胃溃疡

Abstract: Objective To investigate the expressions of interleukin (IL)-6, IL-8 in normal gastric tissue, gastric ulcer and gastric cancer. Methods The plasma expressions of IL-6, IL-8 were determined by enzymelinked immunosorbent assay in 33 normal controls, 30 gastric ulcer patients and 52 gastric cancer patients (preparing for the surgery), and the expressions of IL-6, IL-8 were followed up at the first week after operation. The expressions of IL-6, IL-8 in 45 normal mucosa tissues, 35 gastric ulcer tissues and 45 gastric cancer tissues were determined by immunohistochemical method. Results The plasma expression levels of IL-6, IL-8 were significantly higher in patients with gastric cancer (preoperation and postoperation) than normal controls and gastric ulcer patients(all P＜0.01), however the expression levels decreased after operation (P＜0.01). The positive expression rates of IL-6, IL-8 increased in turn in normal tissues, gastric ulcer tissues and gastric cancer tissues, and the differences were statistically significant (χ2 =38.87, P＜0.01; χ2 =42.23, P＜0.01). Conclusion IL-6 and IL-8 are excessive expressed in the plasma and pathological tissue of gastric cancer patient, and the detection of IL-6 and IL-8 contributes to the judgment of patient′s  condition and prognosis estimation.

Key words: Interleukin-6, Interleukin-8, Stomach neoplasms, Stomach ulcer