The expressions and clinical significances of HOXB1 and miR-3175 in human glioma

doi:10.3760/cma.j.issn.1673-422X.2016.11.004

Abstract

Abstract: ObjectiveTo explore the relationship and the clinical significance of homeobox gene B1 (HOXB1) and microRNA3175 (miR3175) expressions in human glioma. MethodsThe expression levels of HOXB1 and miR3175 in 60 glioma tissues and 15 normal brain tissues were analyzed by realtime fluorescent quantitative PCR (qRTPCR). Spearman rank correlation analysis was performed to explore the relationship between HOXB1 and miR3175 in human glioma tissues. The relationship between HOXB1 (or miR3175) and clinical pathological characteristics of glioma patients was analyzed. The correlation of HOXB1 (or miR3175) and survival rate was calculated by KaplanMeier. And COX regression models were used to assess the prognostic factors. ResultsCompared with normal brain tissues, the expression of HOXB1 was significantly decreased in glioma tissues (1.498±0.323 vs. 0.946±0.588, t=-5.680, P=0.000)； and the expression of miR3175 was obviously increased in glioma tissues (1.008±0.355 vs. 2.076±0.841, t=4.274, P=0.000), and HOXB1 expression was negatively correlated with miR3175 expression in glioma tissues (r=-0.601, P=0.000). HOXB1 expression was related with histologic grade (χ2=4.848, P=0.028), and miR3175 expression was related with histologic grade (χ2=5.640, P=0.018) and Karnofsky score (χ2=4.785, P=0.029）. The results of KaplanMeier revealed that there were significant differences in median survival time between HOXB1 (or miR3175) highexpression group and HOXB1 (or miR3175) lowexpression group ［HOXB1: (21.0±4.0)months vs. (7.0±0.8) months; χ2=7.495, P=0.006; miR3175: (6.0±0.6) months vs. (16.0±5.8) months; χ2=9.591, P=0.002］. COX regression models showed that tumor degree (RR=6.556, 95%CI: 1.19635.952, P=0.002), HOXB1 (RR=0.018, 95%CI: 0.0010.312, P=0.006) and miR3175 (RR=2.098, 95%CI: 1.6637.513, P=0.037) were independent prognostic factors for prognosis. ConclusionThe HOXB1 expression may be negatively correlated with miR3175 in human glioma tissues, and the expression levels of HOXB1 and miR3175 are associated with the glioma malignant degree, survival time and prognosis of glioma patients.

Key words: Glioma, Prognosis, MicroRNAs, Homeobox gene B1

Zhou Haixia, Han Liang, Zhu Zifeng, Wei Jun, Tian Yu, Li Zhaohui. The expressions and clinical significances of HOXB1 and miR-3175 in human glioma[J]. Journal of International Oncology, 2016, 43(11): 817-821.

References

［1］ Bhatlekar S, Fields JZ, Boman BM. HOX genes and their role in the development of human cancers［J］. J Mol Med, 2014, 92(8): 811-823. DOI: 10.1007/s00109-014-1181-y. ［2］ Weiss FU, Marques IJ, Woltering JM, et al. Retinoic acid receptor antagonists inhibit miR-10a expression and block metastatic behavior of pancreatic cancer［J］. Gastroenterology, 2009, 137(6): 21362145.e1-7. DOI: 10.1053/j.gastro.2009.08.065. ［3］ Petrini M, Felicetti F, Bottero L, et al. HOXB1 restored expression promotes apoptosis and differentiation in the HL60 leukemic cell line［J］. Cancer Cell Int, 2013, 13(1): 101. DOI: 10.1186/1475-2867-13-101. ［4］ Morgan R, Boxall A, Harrington KJ, et al. Targeting the HOX/PBX dimer in breast cancer［J］. Breast Cancer Res Treat, 2012, 136(2): 389-398. DOI: 10.1007/s10549-012-2259-2. ［5］ Parafioriti A, Bason C, Armiraglio E, et al. Ewing′s sarcoma: an analysis of miRNA expression profiles and target genes in ParaffinEmbedded primary tumor tissue［J］. Int J Mol Sci, 2016, 17(5): 10. DOI: 10.3390/ijms17050656. ［6］崔运福, 郝涛, 王荣华, 等. 靶向survivin基因微小RNA真核表达载体的构建及其对人结肠癌细胞增殖的影响［J］. 国际肿瘤学杂志, 2015, 42(1): 22-26. DOI: 10.3760/cma.j.issn.1673-422X.2015.01.006. ［7］马可, 周海霞, 田男, 等. 人胶质瘤细胞中微小RNA3175对同源异型盒基因家族成员B1基因表达的调控作用［J］. 中华实验外科杂志, 2015, 32(9): 20642067. DOI: 10.3760/cma.j.issn.1001-9030. ［8］ AbateShen C. Deregulated homeobox gene expression in cancer: cause or consequence?［J］. Nat Rev Cancer, 2002, 2(10): 777-785. DOI: 10.1038/nrc907. ［9］ Shah N, Sukumar S. The Hox genes and their roles in oncogenesis［J］. Nat Rev Cancer, 2010, 10(5): 361-371. DOI: 10.1038/nrc2826. ［10］ Zigman M, LaumannLipp N, Titus T, et al. Hoxb1b controls oriented cell division, cell shape and microtubule dynamics in neural tube morphogenesis［J］. Development, 2014, 141(3): 639-649. DOI: 10.1242/dev.098731. ［11］ Choe SK, Lu P, Nakamura M, et al. Meis cofactors control HDAC and CBP accessibility at Hoxregulated promoters during zebrafish embryogenesis［J］. Dev Cell, 2009, 17(4): 561-567. DOI: 10.1016/j.devcel.2009.08.007. ［12］ Xu LX, Li ZH, Tao YF, et al. Histone acetyltransferase inhibitor Ⅱ induces apoptosis in glioma cell lines via the p53 signaling pathway［J］. J Exp Clin Cancer Res, 2014, 33: 108. DOI: 10.1186/s13046-014-0108-3.

[1]	Qian Xiaotao, Shi Ziyi, Hu Ge, Wu Xiaowei. Efficacy of consolidation chemotherapy after radical radiotherapy and chemotherapy for stage Ⅲ-ⅣA esophageal squamous cell carcinoma： a real-world clinical study [J]. Journal of International Oncology, 2024, 51(6): 326-331.
[2]	Yang Mi, Bie Jun, Zhang Jiayong, Deng Jiaxiu, Tang Zuge, Lu Jun. Analysis of the efficacy and prognosis of neoadjuvant therapy for locally advanced resectable esophageal cancer [J]. Journal of International Oncology, 2024, 51(6): 332-337.
[3]	Fan Zhipeng, Yu Jing, Hu Jing, Liao Zhengkai, Xu Yu, Ouyang Wen, Xie Conghua. Predictive value of changes in inflammatory markers for prognosis in patients with advanced non-small cell lung cancer treated with the first-line immunotherapy plus chemotherapy [J]. Journal of International Oncology, 2024, 51(5): 257-266.
[4]	Yang Lin, Lu Ning, Wen Hua, Zhang Mingxin, Zhu Lin. Study on the clinical relationship between inflammatory burden index and gastric cancer [J]. Journal of International Oncology, 2024, 51(5): 274-279.
[5]	Liu Pingping, He Xuefang, Zhang Yi, Yang Xu, Zhang Shanshan, Ji Yifei. Risk factors of postoperative recurrence in patients with primary brain glioma and prediction model construction [J]. Journal of International Oncology, 2024, 51(4): 193-197.
[6]	Wan Fang, Yang Gang, Li Rui, Wan Qijing. Expression levels and clinical significance of serum miR-497 and miR-383 in patients with esophageal cancer [J]. Journal of International Oncology, 2024, 51(4): 204-209.
[7]	Yao Yixin, Shen Yulin. Predictive value of serum SOCS3 and TXNIP levels for the prognosis of patients with hepatocellular carcinoma treated with TACE [J]. Journal of International Oncology, 2024, 51(4): 217-222.
[8]	Sun Weiwei, Yao Xuemin, Wang Pengjian, Wang Jing, Jia Jinghao. Exploration of prognostic factors and nomogram construction for advanced non-small cell lung cancer treated with immunotherapy based on hematologic indexes [J]. Journal of International Oncology, 2024, 51(3): 143-150.
[9]	Liu Yulan, Jing Haiyan, Sun Jing, Song Wei, Sha Dan. Advances in predicting efficacy and prognostic markers of immunotherapy for gastric cancer [J]. Journal of International Oncology, 2024, 51(3): 175-180.
[10]	Peng Qin, Cai Yuting, Wang Wei. Advances on KPNA2 in liver cancer [J]. Journal of International Oncology, 2024, 51(3): 181-185.
[11]	Gong Yan, Chen Honglei. Research progress on the mechanism of microRNA regulation of cisplatin resistance in ovarian cancer [J]. Journal of International Oncology, 2024, 51(3): 186-190.
[12]	Chen Boguang, Wang Sugui, Zhang Yongjie. Role of serum cholinesterase and inflammatory markers in the prognosis of stage ⅠA -ⅢA breast cancer [J]. Journal of International Oncology, 2024, 51(2): 73-82.
[13]	Jin Xudong, Chen Zhongjian, Mao Weimin. Research progress on the role of MTAP in malignant mesothelioma [J]. Journal of International Oncology, 2024, 51(2): 99-104.
[14]	Huang Zhen, Chen Yongshun. Research progress of circulating tumor DNA in the diagnosis and treatment of hepatocellular carcinoma [J]. Journal of International Oncology, 2024, 51(1): 59-64.
[15]	Wang Xiao, Li Ying, Luo Yujie, Jin Shu. Study on the prognostic value of serological indicators for nasopharyngeal carcinoma based on nomogram model [J]. Journal of International Oncology, 2023, 50(8): 463-469.