Expressions of LSD1 and PDPN in tongue squamous cell carcinoma and their effects on prognosis

doi:10.3760/cma.j.cn371439-20200221-00016

Abstract

Abstract:

Objective To investigate the expressions of lysine specific demethylase 1 (LSD1) and podoplanin (PDPN) in tongue squamous cell carcinoma and the correlation between LSD1 or PDPN and clinicopathological characteristics or prognosis. Methods A total of 67 cases of tongue squamous cell carcinoma and corresponding paracancerous normal tissues in the First Affiliated Hospital of Xinjiang Medical University from January 2011 to January 2016 were selected. The expressions of LSD1 and PDPN in cancer and paracancerous tissues were detected by immunohistochemical method, and the patients were followed up for a long time to analyze the correlation between the expression of LSD1 or PDPN and clinicopathological characteristics or prognosis. Results The expressions of LSD1 and PDPN in tongue squamous cell carcinoma tissues were higher than those in paracancerous tissues, and the differences were statistically significant (Z=6.089, P<0.001; Z=5.781, P<0.001). The expression intensities of LSD1 and PDPN were significantly different in patients with different clinical stage (χ ²=11.487, P=0.001; χ ²=8.111, P=0.004), lymph node metastasis (χ ²=4.772, P=0.029; χ ²=6.206, P=0.013) and tumor size (χ ²=5.985, P=0.014; χ ²=4.247, P=0.039). The expression intensity of LSD1 was also significantly different in patients with different degrees of differentiation (χ ²=6.660, P=0.010). In univariate analysis, LSD1 expression intensity was negatively correlated with progression-free survival (PFS) and overall survival (OS) (χ ²=18.930, P<0.001; χ ²=16.257, P<0.001), PDPN expression intensity was negatively correlated with PFS and OS (χ ²=31.720, P<0.001; χ ²=18.390, P<0.001), and tumor size was negatively correlated with PFS and OS (χ ²=5.326, P=0.021; χ ²=8.843, P=0.003). Postoperative radiotherapy and clinical stage were positively and negatively correlated with OS respectively (χ ²=4.197, P=0.040; χ ²=6.355, P=0.012). In multivariate analysis, LSD1 was an independent risk factor for PFS and OS (HR=5.743, 95%CI: 1.012-32.579, P=0.048; HR=17.759, 95%CI: 2.303-136.916, P=0.006), PDPN was an independent risk factor for PFS (HR=4.380, 95%CI: 1.258-15.254, P=0.020), postoperative radiotherapy was a protective factor for PFS and OS (HR=0.374, 95%CI: 0.157-0.895, P=0.027; HR=0.218, 95%CI: 0.091-0.521, P=0.001), and clinical stage was an independent risk factor for OS (HR=2.637, 95%CI: 1.107-6.280, P=0.029). In tongue squamous cell carcinoma tissues, the expression of LSD1 was positively correlated with that of PDPN (rs=0.655, P<0.001). Conclusion The expressions of LSD1 and PDPN in tongue squamous cell carcinoma are higher than those in adjacent tissues. LSD1 is an independent risk factor for PFS and OS, PDPN is an independent risk factor for PFS, clinical stage is an independent risk factor for OS, and postoperative radiotherapy is a protective factor for PFS and OS. There is a positive correlation between the expressions of LSD1 and PDPN in tongue squamous cell carcinoma, and they can both be used as independent predictors of prognosis in patients with tongue squamous cell carcinoma.

Key words: Tongue neoplasms, Prognosis, Lysine specific demethylase 1, Podoplanin

Wang Kunlong, Zhang Yang, Su Weipeng, Liu Pan, Zhao Huarong. Expressions of LSD1 and PDPN in tongue squamous cell carcinoma and their effects on prognosis[J]. Journal of International Oncology, 2020, 47(5): 264-271.

Figures/Tables 12

References 17

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组别	阴性	弱阳性	阳性	强阳性
癌组织	9	13	35	10
癌旁组织	53	7	3	4
Z值	6.089
P值	<0.001

组别	阴性	弱阳性	阳性	强阳性
癌组织	15	16	30	6
癌旁组织	56	3	7	1
Z值	5.781
P值	<0.001

临床病理特征	无进展生存率						总生存率
临床病理特征	1年	3年	5年	χ²值	P值		1年	3年	5年	χ²值	P值
性别
男	67.6	58.8	52.6	0.116	0.734	82.4		67.6	58.1	0.015	0.902
女	69.7	51.5	48.5			81.8		63.6	59.1
年龄(岁)
≤60	68.2	56.8	52.2	0.042	0.837	84.1		68.2	59.6	0.139	0.710
>60	69.6	52.2	47.8			78.3		60.9	56.5
肿瘤大小
T_1-2	75.0	58.3	58.3	5.326	0.021	91.7		77.1	67.2	8.843	0.003
T_3-4	52.6	36.8	30.7			57.9		36.8	36.8
分化程度
低、中	55.6	51.9	51.9	0.154	0.694	70.4		55.6	51.3	1.580	0.209
高	77.5	57.5	49.6			90.0		72.5	63.9
淋巴结转移
无	76.1	58.7	54.3	1.437	0.231	93.5		71.7	60.9	1.530	0.216
有	52.4	47.6	42.9			57.1		52.4	52.4
临床分期
Ⅰ~Ⅱ	80.0	65.7	60.0	3.718	0.054	100.0		82.9	69.0	6.355	0.012
Ⅲ~Ⅳ	56.3	43.8	40.4			62.5		46.9	46.9
术后化疗
否	68.1	53.2	46.6	0.680	0.410	83.0		61.7	56.4	0.299	0.585
是	70.0	60.0	60.0			80.0		70.0	63.6
术后放疗
否	56.7	43.3	40.0	3.266	0.071	76.7		50.0	45.5	4.197	0.040
是	78.4	64.9	59.2			86.5		78.4	69.3
LSD1表达
低	95.5	90.9	90.9	18.930	<0.001	100.0		100.0	93.3	16.257	<0.001
高	55.6	35.6	30.8			73.3		48.9	41.5
PDPN表达
低	93.5	87.1	87.1	31.720	<0.001	100.0		90.3	87.1	18.390	<0.001
高	47.2	27.8	19.4			66.7		44.4	34.6

表达强度	LSD1						PDPN
表达强度	中位PFS	χ²值	P值	中位OS	χ²值	P值	中位PFS	χ²值	P值	中位OS	χ²值	P值
阴性	-			-	19.752	<0.001	-	31.769	<0.001	-	18.428	<0.001
弱阳性	-	20.260	<0.001	-			-			-
阳性	17			40			12			26
强阳性	9			12			6			9

临床病理因素	B值	SE值	Wald值	P值	HR值	95%CI
术后放疗	-0.982	0.445	4.881	0.027	0.374	0.157~0.895
LSD1表达强度	1.748	0.886	4.051	0.048	5.743	1.012~32.579
PDPN表达强度	1.477	0.637	5.383	0.020	4.380	1.258~15.254