国际肿瘤学杂志

国际肿瘤学杂志 ›› 2013, Vol. 40 ›› Issue (3): 232-235.

• 论著 • 上一篇    下一篇

白藜芦醇对不同乳腺癌细胞增殖的抑制作用

徐杰, 许晓群, 邹志孟, 苏庆红, 张建华, 王郡甫   

  1. 250062济南，山东省医学科学院基础医学研究所 济南大学山东省医学科学院医学与生命科学学院（徐杰、许晓群、苏庆红、张建华、王郡甫）；烟台市牟平区人民医院（邹志孟）

  • 出版日期:2013-03-08 发布日期:2013-02-25
  • 基金资助:

    山东省自然科学基金资助项目（ZR2009CM036）；（ZR2010CM067）；山东省优秀中青年科学家科研奖励基金计划（BS2009SW007）

Inhibition of resveratrol on proliferation of different breast cancer cell lines

XU  Jie, XU  Xiao-Qun, ZOU  Zhi-Meng, SU  Qing-Hong, ZHANG  Jian-Hua, WANG  Jun-Fu   

  1. Institute of Basic Medicine, Shandong Academy of Medical Sciences, Jinan University School of Medical and Life Sciences, Jinan 250062, China

  • Online:2013-03-08 Published:2013-02-25

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1402

被引次数

5

摘要：  目的  探讨白藜芦醇（Res）对雌激素受体表达不同的3种乳腺癌细胞DU4475、MDA-MB-231和MCF-7增殖、周期和凋亡的影响。方法  药物处理组加入终浓度为6.25、12.5、25、50、100、200 μmol/L的Res，分别设24、48、72 h3个时期。采用噻唑蓝比色法（MTT）法检测Res对乳腺癌细胞增殖的影响，流式细胞术(FCM)检测细胞周期及凋亡。药物浓度为0、12.5、25、50μmol/L。应用统计软件SPSS17.0进行统计学处理。结果  经Res作用后，3种乳腺癌细胞增殖均受到不同程度抑制。其中Res作用48 h和72 h，各浓度对细胞的抑制均有统计学意义（F=15.26，P﹤0.05），对雌激素受体（ER）阴性DU4475和MDA-MB-231细胞株的抑制率均高于ER阳性的MCF-7细胞株。FCM检测结果显示，Res与乳腺癌细胞作用48 h，Res浓度在12.5～50 μmol/L时，3种乳腺癌细胞周期均被阻滞于S期，阻滞比例差异有统计学意义(F=34.81，P﹤0.05)，DU4475和MDA-MB-231阻滞于S期的比例高于MCF-7。100μmol/L时，Res对DU4475的促凋亡作用明显强于MCF-7细胞（t=16.082，P﹤0.05）。结论  Res可抑制以上3种乳腺癌细胞增殖，导致细胞周期改变，促进凋亡。Res对DU4475和MDA-MB-231细胞增殖抑制作用强于ER阳性MCF-7细胞。

关键词: 乳腺肿瘤, 细胞系, 肿瘤, 受体, 雌激素, 白藜芦醇

Abstract: Objective  To investigate the effects of resveratrol (Res) on the proliferation, cell cycle and apoptosis of three breast cell lines DU4475, MDA-MB-231 and MCF-7 with different estrogen receptor (ER) expressions. Methods  Res was added to the drug treatment group at 6.25, 12.5, 25, 50, 100 and 200 μmol/L, respectively. Three observation periods at 24, 48 and 72 hours were set up respectively. MTT assay was used to detect the effects of Res on proliferation of breast cells. Cell cycle and apoptosis were analyzed by flow cytometry (FCM). The concentrations of drugs were 0, 12.5, 25, 50 μmol/L. The results were analysed by the statistical software SPSS17.0. Results  After Res intervention, the proliferations of three cell lines were inhibited to different extent. After 48 and 72 hours of Res, inhibitions of Res with different concentration were significant different (F=15.26, P﹤0.05). Inhibition rates of DU4475 and MDA-MB-231 with ER-negative were higer than that of MCF-7 with ER-positive. FCM results prompted that these three kinds of cells were blocked in S phase after 48 hours treatment of 12.5-50 μmol/L Res and the block percentages had significant difference (F=34.81, P﹤0.05). The percentages of S phase for DU4475 and MDA-MB-231 arresting were higher than that of MCF-7. For DU4475, the apoptotic and necrosis percentage were higher than that of MCF-7 at 100 μmol/L (t=16.082, P<0.05). Conclusion  Res can inhibit proliferation, induce cell cycle changing and apoptosis of DU4475, MDA-MB-231 and MCF-7 cells. The inhibitions of Res on DU4475 and MDA-MB-231 are better than that of MCF-7 with ER-positive.

Key words: Breast neoplasms, Cell line, tumor, Receptors, estrogen, Resveratrol