国际肿瘤学杂志

国际肿瘤学杂志 ›› 2017, Vol. 44 ›› Issue (6): 428-.doi: 10.3760/cma.j.issn.1673-422X.2017.06.007

• 论著 • 上一篇    下一篇

NRP-1在肝细胞癌组织中的表达及其临床意义

陈域,纪洪辰,曹大勇,李霄   

  1. 710054 西安，解放军第451中心医院普通外科（陈域）；第四军医大学第一附属医院外科（纪洪辰、曹大勇、李霄）
  • 出版日期:2017-06-08 发布日期:2017-06-16
  • 通讯作者: 李霄 E-mail:lixiao0076@163.com

Expression and clinical value of NRP-1 in hepatocellular carcinoma

Chen Yu, Ji Hongchen, Cao Da yong, Li Xiao   

  1. Department of General Surgery, No. 451 Center Hospital of PLA, Xi′an 710054, China
  • Online:2017-06-08 Published:2017-06-16
  • Contact: Li Xiao E-mail:lixiao0076@163.com

PDF

777

摘要： 目的明确神经纤毛蛋白质1（NRP1）在肝细胞癌（HCC）组织中的表达情况及其临床意义。方法收集151例HCC组织和89例正常肝组织标本，应用免疫组织化学染色法检测其中NRP1蛋白的表达情况。单因素及多因素统计分析NRP1表达与HCC患者临床病理指标的关系，并以生存分析比较不同NRP1表达水平患者的生存情况。结果失访或随访期间因非HCC疾病死亡的病例共11例，最终进入研究的有效病例为140例。NRP1在HCC组织和正常肝组织中的阳性表达率分别为65.00%、35.96%，差异有统计学意义（χ2=18.843，P<0.001）。按照NRP1的表达水平，将140例HCC患者分为阴性表达组49例，阳性表达组91例。单因素分析结果显示，NRP1在HCC中的表达与肿瘤数目（χ2=8.025，P=0.005）、TNM分期（χ2=26.467，P<0.001）、分化程度（χ2=15.296，P<0.001）、门脉侵袭（χ2=9.054，P=0.003）以及肝静脉侵袭（χ2=5.928，P=0.015）有关。多因素分析结果显示，TNM分期（OR=1.392，95%CI为1.121～1.730，χ2=8.950，P=0.003）、分化程度（OR=1.469，95%CI为1.102～1.958，χ2=6.862，P=0.009）、门脉侵袭（OR=1.829，95%CI为1.157～2.893，χ2=6.665，P=0.010）及肝静脉侵袭（OR=2.161，95%CI为1.172～3.987，χ2=6.084，P=0.014）是NRP1表达的影响因素。NRP1阴性组HCC患者的中位生存时间显著长于阳性组患者（44个月∶23个月），差异有统计学意义（χ2=21.922，P<0.001）。结论NRP1在HCC组织中呈过表达趋势，与HCC的恶性程度密切相关，其表达增高提示患者预后不良。

关键词: 癌, 肝细胞, 神经纤毛蛋白质1, 存活率, 基因表达

Abstract: ObjectiveTo determine the expression and clinical value of neuropilin1 (NRP1) in hepatocellular carcinoma (HCC). MethodsOne hundred and fiftyone cases of HCC tissues and 89 cases of healthy liver tissues were chosen to compare the expression of NRP1 by immunohistochemistry. Then the relationships between different clinical factors and the expression of NRP1 were analyzed by univariate and multivariate statistical analysis. Moreover, the survival rates were compared by survival analysis between different expressions of NRP1 in HCC patients. ResultsEleven cases were lost to followup or died for non HCC disease, and the effective cases in the final study were 140 cases. The positive expression rates of NRP1 in HCC and normal liver tissues were 65.00% and 35.96% respectively, and the difference was statistically significant (χ2=18.843, P<0.001). According to the expression level of NRP1, 140 patients with HCC were divided into negative expression group (n=49) and positive expression group (n=91). Univariate analysis showed that the expression of NRP1 in HCC was correlated with tumor number (χ2=8.025, P=0.005), TNM stage (χ2=26.467, P<0.001), differentiation degree (χ2=15.296, P<0.001), portal vein invasion (χ2=9.054, P=0.003) and hepatic vein invasion (χ2=5.928, P=0.015). Multivariate statistical analysis showed that TNM stage (OR=1.392, 95%CI: 1.1211.730, χ2=8.950, P=0.003), differentiation degree (OR=1.469, 95%CI: 1.1021.958, χ2=6.862, P=0.009), portal vein invasion (OR=1.829, 95%CI: 1.1572.893, χ2=6.665, P=0.010) and hepatic vein invasion (OR=2.161, 95%CI: 1.1723.987, χ2=6.084, P=0.014) were important factors for NRP1 expression. The median survival time of NRP1 negative HCC patients was significantly longer than that of positive group (44 months vs. 23 months), and the difference was statistically significant (χ2=21.922, P<0.001). ConclusionNRP1 is overexpression in HCC tissue and related to the malignant progress of HCC, and this suggests poor prognosis in patients with HCC.

Key words: Carcinoma, hepatocellular, Neuropilin-1, Survival rate, Gene expression