国际肿瘤学杂志

国际肿瘤学杂志 ›› 2019, Vol. 46 ›› Issue (9): 526-530.doi: 10.3760/cma.j.issn.1673-422X.2019.09.003

• 论著 • 上一篇    下一篇

miR-205及miR-221在结肠癌患者血清中的水平及其临床意义

赵高传,陈卿奇,庞莉,程正,麦芳奇   

  1. 海南西部中心医院消化内科，儋州571799
  • 收稿日期:2019-05-23 出版日期:2019-09-08 发布日期:2019-09-08
  • 通讯作者: 赵高传 E-mail: zhaogaochuan1@163.com

Serum levels and clinical significances of miR-205 and miR-221 in patients with colon cancer

Zhao Gaochuan, Chen Qingqi, Pang Li, Cheng Zheng, Mai Fangqi   

  1. Department of Gastroenterology, Central Hospital of Western Hainan, Danzhou 571799, China
  • Received:2019-05-23 Online:2019-09-08 Published:2019-09-08
  • Contact: Zhao Gaochuan E-mail: zhaogaochuan1@163.com

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摘要： 目的 探讨结肠癌患者血清微小RNA-205（miR-205）及微小RNA-221（miR-221）的水平及其临床意义。方法 选取2016年1月至2018年12月海南西部中心医院收治的172例结肠癌患者（结肠癌组）、130例结肠良性疾病（良性病变组）和70例健康体检正常者（对照组），检测各组血清miR-205及miR-221水平，分析其水平与结肠癌患者临床病理特征的关系。应用受试者工作特征（ROC）曲线分析血清miR-205及miR-221水平对结肠癌的诊断价值。采用Pearson相关分析探讨血清miR-205及miR-221水平的相关性。结果 结肠癌组、良性病变组和对照组血清miR-205水平分别为2.84±0.96、1.16±0.27、1.05±0.23，差异有统计学意义（F=10.113，P＜0.001），血清miR-221水平分别为1.95±0.74、0.37±0.08、0.32±0.05，差异有统计学意义（F=12.416，P＜0.001）；进一步两两比较发现，结肠癌组血清miR205和miR221水平均高于良性病变组和对照组（均P＜0.001）。结肠癌患者血清miR-205及miR-221水平与患者TNM分期（t＝5.412，P＜0.001；t＝6.103，P＜0.001）、分化程度（t＝4.573，P=0.028；t＝4.805，P=0.013）和有无淋巴结转移（t＝5.837，P＜0.001；t＝7.410，P＜0.001）相关。ROC曲线分析显示，采用血清miR-205及miR-221水平诊断结肠癌的最佳截断值分别为2.17、1.30，两项联合诊断结肠癌的曲线下面积最大（0.924，95%CI为0.865～0.983），其敏感性和特异性分别为91.3%和87.4%。相关分析显示，结肠癌患者血清miR-205与miR-221水平呈正相关（r=0.837，P<0.001）。结论 血清miR-205及miR-221水平在结肠癌患者中明显升高，两项联合检测对结肠癌诊断具有较高的价值。

关键词: 结肠肿瘤, miR-205, miR-221

Abstract: Objective To investigate the serum levels and clinical significances of microRNA-205 (miR-205) and microRNA-221 (miR-221) in patients with colon cancer. Methods A total of 172 patients with colon cancer (colon cancer group), 130 patients with benign diseases of colon (benign lesion group) and 70 healthy persons (control group) admitted to Central Hospital of Western Hainan from January 2016 to December  2018 were selected. The serum levels of miR-205 and miR-221 in each group were detected, and their relationships with clinicopathological characteristics of patients with colon cancer were analyzed. The diagnostic values of the serum levels of miR-205 and miR-221 in colon cancer were analyzed by receiver operating characteristic (ROC) curve. Pearson correlation analysis was used to analyze the correlation between the serum levels of miR-205 and miR-221. Results The serum levels of miR-205 in colon cancer group, benign lesion group and control group were 2.84±0.96, 1.16±0.27 and 1.05±0.23, with a statistically significant difference (F=10.113, P＜0.001). The serum levels of miR-221 in the three groups were 1.95±0.74, 0.37±0.08 and 0.32±0.05, with a statistically significant difference (F=12.416, P＜0.001). Further pairwise comparisons found that the serum levels of miR-205 and miR-221 in colon cancer group were significantly higher than those in benign lesion group and control group (all P＜0.001). The serum levels of miR-205 and miR-221 in patients with colon cancer were related with TNM stage (t＝5.412, P＜0.001; t＝6.103, P＜0.001), degree of tumor differentiation (t＝4.573, P=0.028; t＝4.805, P=0.013) and with or without lymph node metastasis (t＝5.837, P＜0.001; t＝7.410, P＜0.001). ROC curve analysis showed that the optimal cutoff values of the serum levels of miR-205 and miR-221 for the diagnosis of colon cancer were 2.17 and 1.30, respectively. The area under the curve of the two combined diagnostic method for colon cancer was the largest (0.924, 95%CI: 0.865-0.983), with the sensitivity and specificity of 91.3% and 87.4%. The correlation analysis showed that the serum levels of miR-205 and miR-221 were positively correlated in patients with colon cancer (r=0.837, P<0.001). Conclusion The serum levels of miR-205 and miR-221 are significantly increased in patients with colon cancer, and the two combined detection has a high value for the diagnosis of colon cancer.

Key words: Colonic neoplasms, miR-205, miR-221