NDRG2基因对结肠癌细胞SW620增殖和侵袭力的影响

doi:10.3760/cma.j.issn.1673-422X.2016.06.001

摘要/Abstract

摘要： 目的观察N-myc下游调节基因2（NDRG2）对结肠癌细胞SW620生长和侵袭能力的影响，并探讨其机制。方法采用阳离子脂质体转染方法，分别将pcDNA3.1NDRG2和siRNANDRG2转染入SW620细胞内，以空白组作为对照。Western blotting检测各组细胞NDRG2以及基质金属蛋白酶-2（MMP-2）的表达情况；细胞侵袭试验对各组细胞侵袭能力进行分析；四甲基偶氮唑蓝法对各组细胞生长曲线进行测定。结果 pcDNA3.1NDRG2转染入SW620细胞后，NDRG2蛋白表达升高，而MMP-2蛋白表达降低；siRNANDRG2转染入SW620细胞后，NDRG2蛋白表达降低，而MMP2蛋白表达升高。pcDNA3.1组的穿膜细胞数（56.20±7.40）及siRNA组穿膜细胞数（94.20±9.23）分别与对照组（75.80±4.82）相比，差异具有统计学意义（t=13.102，P=0.000；t=11.820，P=0.000）。生长曲线显示，转染后第5天，pcDNA3.1组细胞吸光度值（0.46±0.01）及siRNA组细胞吸光度值（0.91±0.02）分别与对照组（0.67±0.01）相比，差异具有统计学意义（t=9.561，P=0.000；t=10.922，P=0.000）。结论NDRG2能降低结肠癌细胞SW620的侵袭和增殖能力，其机制可能与下调MMP-2的表达有关。

关键词: 结肠肿瘤, 印迹法, 蛋白质, 转染, NDRG2基因

Abstract: Objective To observe the influence of N-myc downstreamregulated gene 2 (NDRG2) on the growth and invasive ability of human colon cancer cell line SW620, and to explore its mechanism. MethodspcDNA3.1NDRG2 and siRNANDRG2 were transfected transiently respectively into SW620 by Lipofectamine TM 2000, untreated cells as the control group. Western blotting was used to investigate the expression of NDRG2 and matrix metalloproteinase-2(MMP-2). Matrigel invasion assay was used to study the invasive abilities of SW620 cells in all groups. The growth curve was determined through 3(4,5dimethyl2thiazoly)2,5diphenyl2Htetrazolium bromide method. ResultAfter transfecting pcDNA3.1NDRG2 into the SW620 cells, the protein level of NDRG2 increased and the expression of MMP-2 declined markedly. After transfecting siRNANDRG2 into the SW620 cells, the protein level of NDRG2 declined and the expression of MMP2 increased markedly. In addition, compared with the control group (75.80 ±4.82), the numbers of transmembrane cells in pcDNA3.1 group (56.20 ±7.40) and in siRNA group (94.20 ±9.23) were significantly different (t=13.102, P=0.000; t=11.820, P=0.000). The growth curve showed that: compared with the control group (0.67±0.01), the absorbance of the fifth day after transfection in pcDNA3.1 group (0.46±0.01) and in siRNA group (0.91±0.02) were different significantly (t=9.561, P=0.000; t=10.922, P=0.000). ConclusionNDRG2 can reduce the invasion and proliferation ability of colon cancer cell SW620, and its mechanism may be related to the downregulation of MMP-2 expression.

Key words: Colonic neoplasms, Blotting, Western, Transfection, NDRG2 gene

王建勋，曾甜，张鹏，王治刚，雷海录. NDRG2基因对结肠癌细胞SW620增殖和侵袭力的影响[J]. 国际肿瘤学杂志, 2016, 43(6): 401-404.

Wang Jianxun*, Zeng Tian, Zhang Peng, Wang Zhigang, Lei Hailu.. Effects of tumor suppressor NDRG2 gene on proliferation and invasive ability of colon cancer cell line SW620[J]. Journal of International Oncology, 2016, 43(6): 401-404.

参考文献

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