国际肿瘤学杂志

国际肿瘤学杂志 ›› 2018, Vol. 45 ›› Issue (1): 1-.doi: 10.3760/cma.j.issn.1673-422X.2018.01.001

• 论著 •    下一篇

敲低Stat5对甲状腺癌TT细胞侵袭及上皮间质转化的影响

李艳，陈琼霞，王绪明   

  1. 430056 武汉，江汉大学医学院病理学与病理生理学教研室
  • 出版日期:2018-01-08 发布日期:2018-02-12
  • 通讯作者: 李艳 E-mail:ly56880@126.com

Effects of knockdown of Stat5 on invasion and epithelial mesenchymal transition of thyroid carcinoma TT cells

Li Yan, Chen Qiongxia, Wang Xuming   

  1. Department of Pathology and Pathophysiology, School of Medicine, Jianghan University, Wuhan 430056, China
  • Online:2018-01-08 Published:2018-02-12
  • Contact: Li Yan E-mail:ly56880@126.com

被引次数

1

摘要： 目的研究敲低信号转导子和转录激活子5（Stat5）对甲状腺癌TT细胞侵袭及上皮间质转化（EMT）的影响。方法培养甲状腺癌TT细胞并分为Stat5小干扰RNA（siRNA）组和NCsiRNA组，分别转染Stat5的siRNA和阴性对照的NCsiRNA。转染后，采用Transwell模型检测细胞的侵袭数目，采用荧光定量PCR试剂盒测定细胞中基质金属蛋白酶（MMP）家族基因、EMT标志基因、血管新生基因的表达量。结果转染siRNA后12 h、18 h、24 h，Stat5siRNA组细胞的侵袭数目均显著低于NCsiRNA组（42.39±5.82∶64.41±8.49，t＝-4.784，P=0.001；51.23±6.38∶78.54±9.52，t＝-5.329，P＜0.001；60.35±8.35∶98.42±11.25，t＝-6.076，P＜0.001）。转染siRNA后24 h，Stat5siRNA组细胞中MMP1、MMP2、MMP9、MMP13、神经型钙黏蛋白（Ncadherin）、波形蛋白（Vimentin）、血管内皮生长因子（VEGF）、碱性纤维母细胞生长因子（bFGF）、血管生成素（Ang）1、Ang2的mRNA表达量均显著低于NCsiRNA组（0.42±0.07∶1.03±0.15，t＝-8.240，P＜0.001；0.35±0.06∶1.01±0.13，t＝-10.307，P＜0.001；0.29±0.05∶1.05±0.18，t＝-9.097，P＜0.001；0.54±0.08∶0.98±0.12，t＝-6.822，P＜0.001；0.38±0.06∶1.04±0.18，t＝-7.778，P＜0.001；0.29±0.04∶1.06±0.12，t＝-12.612，P＜0.001；0.36±0.07∶1.06±0.14，t＝-10.000，P＜0.001；0.43±0.08∶0.97±0.12，t＝-8.372，P＜0.001；0.25±0.03∶1.03±0.12，t＝-14.100，P＜0.001；0.19±0.03∶0.99±0.13，t＝-13.408，P＜0.001），上皮细胞钙黏蛋白（Ecadherin）的mRNA表达量显著高于NCsiRNA组（2.88±0.42∶0.98±0.12，t＝9.726，P＜0.001）。结论敲低Stat5对甲状腺癌TT细胞侵袭及EMT具有抑制作用。

关键词: 甲状腺肿瘤, 肿瘤侵润, 信号转导子和转录激活子5, 上皮间质转化

Abstract: ObjectiveTo investigate the effects of knockdown of signal transducer and activator of transcription 5 (Stat5) on invasion and epithelial mesenchymal transition (EMT) of thyroid carcinoma TT cells. MethodsThyroid carcinoma TT cells were cultured and divided into Stat5small interfering RNA (siRNA) group and NCsiRNA group, transfected with Stat5 siRNA and negative control NCsiRNA respectively. After transfection, the numbers of invasive cells were determined by Transwell model and the expressions of matrix metalloproteinase (MMP) family genes, EMT marker genes, angiogenesis genes in the cells were determined by fluorescent quantitative PCR. ResultsThe numbers of invasive cells in Stat5siRNA group were significantly lower than those in NCsiRNA group (42.39±5.82 vs. 64.41±8.49, t＝-4.784, P=0.001; 51.23±6.38 vs. 78.54±9.52, t＝-5.329, P＜0.001; 60.35±8.35 vs. 98.42±11.25, t＝-6.076, P＜0.001) after the transfection of siRNA for 12 hours, 18 hours and 24 hours. Twentyfour hours after transfection of siRNA, the mRNA expressions of MMP1, MMP2, MMP9, MMP13, Ncadherin, Vimentin, vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), angiogenin (Ang)1 and Ang2 in Stat5siRNA group were significantly lower than those in NCsiRNA group (0.42±0.07 vs. 1.03±0.15, t＝-8.240, P＜0.001; 0.35±0.06 vs. 1.01±0.13, t＝-10.307, P＜0.001; 0.29±0.05 vs. 1.05±0.18, t＝-9.097, P＜0.001; 0.54±0.08 vs. 0.98±0.12, t＝-6.822, P＜0.001; 0.38±0.06 vs. 1.04±0.18, t＝-7.778, P＜0.001; 0.29±0.04 vs. 1.06±0.12, t＝-12.612, P＜0.001; 0.36±0.07 vs. 1.06±0.14, t＝-10.000, P＜0.001; 0.43±0.08 vs. 0.97±0.12, t＝-8.372, P＜0.001; 0.25±0.03 vs. 1.03±0.12, t＝-14.100, P＜0.001; 0.19±0.03 vs. 0.99±0.13, t＝-13.408, P＜0.001). The mRNA expression of Ecadherin was significantly higher than that in NCsiRNA group (2.88±0.42 vs. 0.98±0.12, t＝9.726, P＜0.001). ConclusionKnockdown of Stat5 inhibits the invasion and EMT of thyroid carcinoma TT cells.

Key words: Thyroid neoplasms, Neoplasm invasiveness, Signal transducer and activator of transcription 5, Epithelial mesenchymal transition