mtMSI及Hp检测对食管鳞状细胞癌患者诊断的临床意义

doi:10.3760/cma.j.issn.1673-422X.2017.10.002

摘要/Abstract

摘要： 目的检测食管鳞状细胞癌线粒体微卫星不稳定性(mtMSI)及幽门螺杆菌（Hp）感染情况，分析其对食管鳞状细胞癌诊断的临床意义。方法应用免疫组织化学SP法检测93例食管鳞状细胞癌组织及其正常对照组织的mtMSI和Hp感染情况，并分析其与食管鳞状细胞癌临床病理特征的关系及两者相关性。结果肿瘤组织和对照组Hp阳性率分别为61.3%（57/93）和20.4%（19/93），差异有统计学意义（χ2=32.127，P＜0.001）；mtMSI阳性率分别为34.4%（32/93）和0（0/93），差异有统计学意义（χ2=38.649，P＜0.001）。Hp感染与肿瘤浸润程度（χ2=22.213，P＜0.001）、淋巴结转移（χ2=8.318，P=0.004）相关，与患者性别（χ2=0.330，P=0.565）、肿块长径（χ2=0.692，P=0.406）、分型（χ2=1.006，P=0.316）、分化程度（χ2=0.665，P=0.415）均无相关性。mtMSI与患者性别（χ2=0.163，P=0.686）、肿块长径（χ2=0.384，P=0.530）、分型（χ2=0.422，P=0.516）、分化程度（χ2=0.213，P=0.645）、浸润程度（χ2=0.001，P=0.979）、淋巴结转移（χ2=0.039，P=0.843）均无相关性。在食管鳞状细胞癌组织中，Hp感染与mtMSI呈正相关(r=0.864，P=0.006)。结论Hp、mtMSI在食管鳞状细胞癌中的阳性率高于正常组织，且两者之间关系密切，Hp还与食管鳞状细胞癌病情的进展具有相关性。

关键词: 食管肿瘤, 幽门螺杆菌, 线粒体微卫星不稳定性

Abstract: ObjectiveTo detect the mitochondrial microsatellite instability (mtMSI) and Helicobacter pylori (Hp) infection status in esophageal squamous cell carcinoma (ESCC), and to analyze their clinical significance in the diagnosis of ESCC. MethodsThe mtMSI and Hp infection status were examined by immunohistochemical SP method in 93 cases of ESCC and their normal control tissues. The relationship between Hp and mtMSI and their correlations with the clinicopathological features of ESCC were analyzed. ResultsThe Hp positive rates of ESCC group and control group were 61.3% (57/93) and 20.4% (19/93) respectively, and there was statistically significant difference between the two groups (χ2=32.127, P＜0.001). The mtMSI positive rates of ESCC group and control group were 34.4% (32/93) and 0 (0/93) respectively, and there was statistically significant difference between the two groups (χ2=38.649, P＜0.001). The Hp infection was correlated with tumor infiltrating degree (χ2=22.213, P＜0.001) and lymph node metastasis (χ2=8.318, P=0.004), but was not correlated with gender (χ2=0.330, P=0.565), major axis of tumor (χ2=0.692, P=0.406), gross type (χ2=1.006, P=0.316), differentiated degree (χ2=0.665, P=0.415). The mtMSI was not correlated with gender (χ2=0.163, P=0.686), major axis of tumor (χ2=0.384, P=0.530), gross type (χ2=0.422, P=0.516), differentiated degree (χ2=0.213, P=0.645), infiltrating degree (χ2=0.001, P=0.979) or lymph node metastasis (χ2=0.039, P=0.843). The Hp infection was positively associated with mtMSI in ESCC (r=0.864, P=0.006). ConclusionThe positive rates of Hp and mtMSI in ESCC are higher than those in normal tissues, and there is a close relationship between Hp and mtMSI. Hp is also associated with the progression of ESCC.

Key words: Esophageal neoplasms, Helicobacter pylori, Mitochondrial microsatellite instability

彭芊芊，邓三花，陈培生，何锋坚，徐胜浩. mtMSI及Hp检测对食管鳞状细胞癌患者诊断的临床意义[J]. 国际肿瘤学杂志, 2017, 44(10): 727-730.

Peng Qianqian, Deng Sanhua, Chen Peisheng, He Fengjian, Xu Shenghao. Clinical significance of detection of mtMSI and Hp in the diagnosis of esophageal squamous cell carcinoma[J]. Journal of International Oncology, 2017, 44(10): 727-730.

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