国际肿瘤学杂志

国际肿瘤学杂志 ›› 2016, Vol. 43 ›› Issue (8): 664-668.doi: 10.3760/cma.j.issn.1673422X.2016.09.006

• 论著 • 上一篇    下一篇

BMI1和PADI4在食管鳞状细胞癌中的表达及其临床意义

季怀君,刘鹏,甄天昌,苏功章,孙宁波,蒋仲敏   

  1. 261053 潍坊医学院研究生院(季怀君)；泰山医学院研究生院（刘鹏）；山东省千佛山医院胸外科（甄天昌、苏功章、蒋仲敏）；胜利油田中心医院胸外科（孙宁波）
  • 出版日期:2016-09-08 发布日期:2016-08-04
  • 通讯作者: 蒋仲敏，Email: qyjzm@sina.cn E-mail:qyjzm@sina.cn
  • 基金资助:

     山东省科技发展计划(2013GSF11836)

Expressions and clinicopathological significances of BMI1 and PADI4 in esophageal squamous cell carcinoma

Ji Huaijun, Liu Peng, Zhen Tianchang, Su Gongzhang, Sun Ningbo, Jiang Zhongmin   

  1. Graduate School, Weifang Medical College, Weifang 261053, China
  • Online:2016-09-08 Published:2016-08-04
  • Contact: Jiang Zhongmin E-mail:qyjzm@sina.cn
  • Supported by:

    Science and Technology Promotion Foundation of Shandong Province of China (2013GSF11836)

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摘要： 【摘要】目的检测食管鳞状细胞癌组织及对应癌旁组织中肽酰基精氨酸脱亚胺酶4（PADI4）和B细胞特异莫洛尼鼠白血病病毒整合位点1（BMI1）的表达，探讨其在食管鳞状细胞癌发生中的作用及临床意义，并探索两者的相关性。方法采用免疫组织化学、Western blotting及实时定量PCR法检测86例食管鳞状细胞癌及配对癌旁组织标本中PADI4和BMI1的表达，并分析其在食管鳞状细胞癌中的表达情况与各临床病理因素的关系。结果免疫组织化学结果显示食管鳞状细胞癌中PADI4、BMI1的阳性表达率分别为68.6%和73.3%，明显高于癌旁组织中的37.2%和30.2%（χ2=17.011，P=0.000；χ2=31.876，P=0.000）；Western blotting显示食管鳞状细胞癌中PADI4、BMI1的表达量显著高于癌旁组织（0.919±0.098∶0.718±0.103，t=2.462，P=0.021；0.975±0.074∶0.717±0.071，t=2.640，P=0.014）；实时定量PCR显示食管鳞状细胞癌中BMI1、PADI4 mRNA相对表达量比对应癌旁组织增高，但差异无统计学意义（0.091±0.005∶0.038±0.002，t=1.701，P=0.101；0.114±0.075∶0.048±0.003，t=1.499，P=0.146）。食管鳞状细胞癌中PADI4表达与肿瘤的淋巴结转移（χ2=5.771，P=0.016）、浸润深度（χ2=6.672，P=0.010）、临床分期（χ2=5.771，P=0.016）密切相关；BMI1表达与淋巴结转移（χ2=7.176，P=0.007）、分化程度（χ2=13.787，P=0.001）、临床分期（χ2=7.176，P=0.007）密切相关。另外，通过免疫组织化学及实时定量PCR检测发现PADI4和BMI1在食管鳞状细胞癌中的表达呈正相关（r=0.214，P=0.047；r=0.534，P=0.005）。结论PADI4和BMI1在食管鳞状细胞癌中表达较癌旁组织均明显升高且呈正相关，其有望成为食管鳞状细胞癌诊断及判断预后的新指标。

关键词: 食管肿瘤, 肽酰基精氨酸脱亚胺酶4, B细胞特异莫洛尼鼠白血病病毒整合位点1

Abstract: 【Abstract】ObjectiveTo evaluate the expression levels of peptidylarginine deiminase 4 (PADI4) and Bcells pecific Moloney leukemia virus insert site1 (BMI1) in esophageal squamous cell carcinoma (ESCC) tissues and pericarcinous tissues. To explore the function and clinical significance in the development of ESCC and their association. MethodsThe expression levels of PADI4 and BMI1 were measured by immunohistochemistry, Western blotting and quantitative real time PCR in ESCC tissues and pericarcinous tissues from 86 patients. The relationships between the expressions of PADI4 and BMI1 and the clinicopathologic characteristics were analyzed. ResultsThe immunohistochemistry showed that the expressions of PADI4 and BMI1 in ESCC tissues (68.6% and 73.3%) were significantly higher than those in pericarcinous tissues (37.2% and 30.2%, χ2=17.011, P=0.000; χ2=31.876, P=0.000). Western blotting indicated that the levels of PADI4 and BMI1 were higher than those in pericarcinous tissues (0.919±0.098 vs. 0.718±0.103, t=2.462, P=0.021; 0.975±0.074 vs. 0.717±0.071, t=2.640, P=0.014). The expressions of BMI1 and PADI4 mRNA in ESCC tissues were higher than those in pericarcinous tissues, but the differences were not statistically significant (0.091±0.005 vs. 0.038±0.002, t=1.701, P=0.101; 0.114±0.075 vs. 0.048±0.003, t=1.499, P=0.146) by the quantitative real time PCR. The expression of PADI4 was correlated with lymph node metastasis (χ2=5.771, P=0.016), depth of invasion (χ2=6.672, P=0.010) and clinical stage (χ2=5.771, P=0.016). The BMI1 gene expression had a correlation with lymph node metastasis (χ2=7.176, P=0.007), the differentiation degree (χ2=13.787, P=0.001) and clinical stage (χ2=7.176, P=0.007). In addition, there was a positive correlation between PADI4 and BMI1 expression in ESCC by immunohistochemistry and quantitative real time PCR (r=0.214, P=0.047; r=0.534, P=0.005). ConclusionThe expression levels of PADI4 and BMI1 are significantly higher in ESCC compared to pericarcinous tissues. PADI4 and BMI1 are positively correlated and may contribute to the diagnosis and prognosis of the ESCC.

Key words: Esophageal neoplasms, Peptidylarginine deiminase 4, Bcells pecific Moloney leukemia virus insert site1