国际肿瘤学杂志

国际肿瘤学杂志 ›› 2015, Vol. 42 ›› Issue (10): 730-733.doi: 10.3760/cma.j.issn.1673-422X.2015.10.003

• 论著 • 上一篇    下一篇

非小细胞肺癌MMP-7和bFGF的表达与间质微血管密度和肿瘤转移的关系

李道胜, 班媛媛   

  1. 271000  山东省泰安市中心医院病理科（李道胜），老年病一科（班媛媛）
  • 出版日期:2015-10-08 发布日期:2015-09-08
  • 通讯作者: 李道胜 E-mail:ldsh19770116@163.com

Expressions of MMP-7 and bFGF and their correlations with tumor interstitial microvascular density and cancer metastasis in NSCLC

Li Daosheng, Ban Yuanyuan   

  1. Department of Pathology, Central Hospital of Taian, Shandong Province, Taian 271000, China
  • Online:2015-10-08 Published:2015-09-08
  • Contact: Li Daosheng E-mail:ldsh19770116@163.com

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摘要： 目的 检测基质金属蛋白酶-7（MMP-7）和碱性成纤维细胞生长因子(bFGF）在非小细胞肺癌（NSCLC）组织中的表达情况，探讨MMP7、bFGF与肿瘤内间质微血管密度（MVD）的关系及MMP-7、bFGF、MVD与肿瘤转移的关系。方法 采用免疫组织化学SP法检测原发性NSCLC石蜡包埋组织80例、正常肺组织40例中MMP-7和bFGF蛋白的表达水平，比较MMP-7、bFGF在NSCLC、正常肺组织中的表达情况及在NSCLC中的表达与淋巴结转移的关系；在CD34染色切片上检测MVD，检测NSCLC中的MVD与MMP-7、bFGF表达的关系及与组织学类型、分化程度、淋巴结转移、TNM分期的关系。。结果 MMP-7和bFGF在NSCLC组的阳性率分别为70.0%、65.0%，均高于正常肺组织组的12.5%和10.0%，差异有统计学意义（χ2=35.276，P＝0.000；χ2=32.411，P＝0.000)；有淋巴结转移组MMP-7和bFGF的阳性表达率分别为83.0%、80.9%，无淋巴结转移组的表达率分别为51.5%和42.4%，差异有统计学意义（χ2=9.139，P＝0.003；χ2=12.584，P＝0.000）；NSCLC中MMP7和bFGF阳性病例组的MVD分别为46.78±11.11、45.05±10.97，高于阴性病例组的31.12±7.62、34.77±8.56，差异有统计学意义（t＝5.831，P＝0.000；t＝5.364，P＝0.000）；有淋巴结转移组的MVD为61.32±21.61，高于无淋巴结转移组的40.52±18.31，差异有统计学意义（t＝3.865,P＝0.008）；TNM分期中Ⅰ～Ⅱ期MVD为41.73±16.48，Ⅲ～Ⅳ期MVD为55.28±15.64，差异有统计学意义(t＝2.531,P＝0.013)。结论 MMP-7与bFGF在NSCLC组织中高表达，其表达的阳性率与淋巴结转移有相关性，且阳性病例组的MVD均显著高于阴性病例组，MVD与淋巴结转移及TNM分期有相关性，提示三者在NSCLC侵袭转移过程中可能起到了重要的作用。

关键词: 癌, 非小细胞肺, 免疫组织化学, 基质金属蛋白酶-7, 碱性成纤维细胞生长因子, 微血管密度

Abstract: Objective To investigate the expressions of matrix metalloproteinase-7 （MMP-7）, basic fibroblast growth factor （bFGF） in NSCLC, and to elucidate the correlations among MMP7, bFGF and MVD, as well as their relationships with cancer metastasis in NSCLC. Methods The expressions of MMP-7, bFGF and MVD were detected by immunohistochemical SP method in 80 cases of NSCLC and 40 cases of normal lung tissue, compare the expression of MMP-7 and bFGF in NSCLC and normal lung tissue, and then invest the relationship between their expression and lymph node metastasis in NSCLC. The MVD was investigated within the CD34 positive slides,  check the relationship between MVD and the expression of MMP-7 , bFGF in NSCLC, and also its relationship with histology type, differentiation degree, lymph node metastasis and TNM stage. Results The positive rates of MMP-7 and bFGF were significantly higher in NSCLC than those in normal lung tissue (70.0% vs 12.5%, χ2＝35.276, P＝0.000; 65.0% vs 10.0%, χ2＝32.411, P＝0.000). The positive rates of MMP-7 and bFGF were also significantly higher in group with lymph node metastasis compared to those group without lymph node metastasis (83.0% vs 51.5%, χ2＝9.139, P＝0.003; 80.9% vs 42.4%, χ2=12.584, P＝0.000). The numbers of MVD were significantly higher in cases with MMP-7 and bFGF positive expressions than those in cases with MMP-7 and bFGF negative expressions (46.78±11.11 vs 31.12±7.62, t＝5.831, P＝0.000; 45.05±10.97 vs 34.77±8.56, t＝5.364, P＝0.000). The number of MVD in group with lymph node metastasis was also significantly higher than that in group without lymph node metastasis (61.32±21.61 vs 40.52±18.31, t＝3.865, P＝0.008). The number of MVD in staging Ⅲ-Ⅳ group was significantly higher than that in stagingⅠ-Ⅱgroup ( 55.28±15.64 vs 41.73±16.48, t＝2.531, P＝0.013). Conclusion The expression levels of MMP-7 and bFGF in NSCLC are high, and the positive rates of MMP-7 and bFGF are correlated with the lymphnode metastasis. The numbers of MVD are significantly higher in cases with MMP7 and bFGF positive expressions than those in cases with MMP7 and bFGF negative expressions. MVD is correlated with lymph node metastasis and the TNM stage. It suggests that the expressions of MMP7, bFGF and MVD may involve in the invasion and metastasis of NSCLC.

Key words: Carcinoma, non-small-cell lung, Immunohistochemistry, Matrix metalloproteinase-7, Basic fibroblast growth factor, Microvascular density