国际肿瘤学杂志

国际肿瘤学杂志 ›› 2014, Vol. 41 ›› Issue (11): 875-878.doi: 10.3760/cma.j.issn.1673-422X.2014.11.023

• 论著 • 上一篇    下一篇

Sox2在人前列腺癌中的表达及其临床意义

  

  1. 河北省唐山市人民医院普外科（张振宇、张宇峰）；河北省唐山市第二医院重症医学科(张宏伟)
  • 出版日期:2014-12-03 发布日期:2015-01-20
  • 通讯作者: 张宏伟，Email：zhanghongweiicu@sina.com

Expression and clinical significance of transcription factor Sox2 in human prostate cancer

  1. Department of General Surgery,   Tangshan People′s Hospital, Tangshan 063000, China
  • Online:2014-12-03 Published:2015-01-20
  • Contact: Zhang Hongwei, Email: zhanghongweiicu@sina.com

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摘要： 目的检测Sox2在人前列腺癌和癌旁组织中的表达情况，并分析其与前列腺癌病理分级和临床分期的关系。方法分别采用免疫组织化学、Western杂交及反转录聚合酶链反应（RTPCR）检测86例前列腺癌组织标本和40例癌旁组织标本中Sox2的表达情况。分析其与前列腺癌病理分级和临床分期之间的相关性。结果免疫组织化学检测结果显示前列腺癌组织中Sox2表达显著高于癌旁组织，阳性表达率分别为79.1%和7.5%，差异有统计学意义(χ2=53.98，P<0.05)；在前列腺癌组织中Sox2的表达与Gleason评分之间相关性具有统计学意义(χ2=19.49，P<0.05)；在淋巴结转移组和非淋巴结转移组Sox2的阳性表达率分别为92.3%和73.3%，差异有统计学意义(χ2=3.95，P<0.05)；但在不同临床分期的前列腺癌组织中Sox2的表达差异无统计学意义（χ2=0.16，P>0.05）。Western杂交及RTPCR检测结果显示前列腺癌组织中Sox2表达显著高于癌旁组织，在淋巴结转移组和非淋巴结转移组之间Sox2表达差异具有统计学意义(t=-2.93，P<0.05； t=3.29，P<0.05)。结论Sox2的表达差异与前列腺癌的Gleason评分和淋巴结转移密切相关,而与临床分期无关，Sox2可能在前列腺癌的发生、分化和转移过程中起重要作用。

关键词: 前列腺肿瘤； 基因表达； Sox2 

Abstract: ObjectiveTo study the expression of Sox2 in human prostate cancer and pericarcinomatous tissue, and the relation between its expression and the pathological type and clinical stage of prostate cancer. MethodsImmunohistochemisty, Western blot and reverse transcriptionpolymerase chain reaction (RTPCR) were used to detect the expression of Sox2 in 86 cases of prostate cancer tissues and 40 cases of pericarcinomatous tissues. The relation between Sox2 expression and the pathological type and clinical stage of prostate cancer was analyzed. ResultsThe expression of Sox2 in prostate cancer tissue (71.9%) was significantly higher than that in pericarcinomatous tissue (7.5%), and the difference was statistically significant (χ2=53.98, P<0.05).  Sox2 expression in prostate cancer tissues was significantly related to Gleason score (χ2=19.49, P<0.05). The positive rate of Sox2 in lymphatic metastasis group and negative lymphatic metastasis group was 92.3％ and 73.3％ respectively, and the difference was statistically significant (χ2=3.95, P<0.05)．While there was no significant difference among clinical stages (χ2=0.16, P>0.05). Moreover, Western blot and RTPCR revealed that the expression of Sox2 in prostate cancer tissue was significantly higher than that in pericarcinomatous tissue (t=-2.93, P<0.05; t=3.29, P<0.05). ConclusionThe high expression of Sox2 is closely related to the Gleason score and lymphatic metastasis of prostate cancer, but not to the clinical stage. The overexpression of Sox2 may play an important role in formation, differentiation and metastasis of prostate cancer.

Key words: Prostatic neoplasms, Gene expression, Sox2