低温等离子体在肿瘤治疗中的应用

doi:10.3760/cma.j.issn.1673-422X.2014.01.008

摘要/Abstract

摘要： 研究表明，低温等离子体对肝癌、黑色素瘤、脑肿瘤、大肠癌、肺癌等具有一定的治疗作用。主要机制为：等离子体作用后使癌细胞内一氧化氮和活性氧水平增高，导致DNA损伤和线粒体功能障碍，细胞内线粒体凋亡通路被激活，改变了Bax/Bcl-2比例，活化凋亡蛋白caspase3、9，伴有细胞周期阻滞（G2-M期或S期），最终导致细胞生长阻滞和细胞凋亡；增加βcatenin磷酸化，导致βcatenin降解，抑制整合蛋白 2、 4以及细胞表面局部黏着斑激酶的表达，破坏细细胞黏附，降低细胞的迁移和侵袭活性。

关键词: 细胞凋亡, 肿瘤治疗方案, 等离子体

Abstract: Research shows that cold plasma has therapeutic effect on hepatocellular carcinoma, melanoma, brain tumors, colorectal cancer, lung cancer and so on. The main mechanisms are as follows: the plasma can increase the levels of nitric oxide and reactive oxygen species in tumor cells, lead to DNA damage and mitochondrial dysfunction, activate mitochondrial apoptosis pathway, change the ratio Bax/Bcl-2, activate apoptosis proteins caspase3, 9, accompanied by cell cycle arrest (G2-M phase or S phase), eventually induce cell growth arrest and apoptosis; increase βcatenin phosphorylation, lead to βcatenin degradation, inhibit the expressions of integrin α2, integrin α4 and focal adhesion kinase on the cell surface, destroy cancer cells adhesion and reduce cell migration and invasion activities.

Key words: Apoptosis, Antineoplastic protocols, Plasma

胡超, 周菊英. 低温等离子体在肿瘤治疗中的应用[J]. 国际肿瘤学杂志, 2014, 41(1): 24-27.

Hu Chao, Zhou Juying. Application of cold plasma in cancer therapy[J]. Journal of International Oncology, 2014, 41(1): 24-27.

参考文献

［1］ Morfill GE, Kong MG, Zimmermann JL. Focus on plasma medicine［J］. New J Phys, 2009, 11:115011. ［2］ Stoffels E, Sakiyama Y, Graves DB. Cold atmospheric plasma: charged species and their interactions with cells and tissues［J］. IEEE Trans Plasma Sci, 2008, 36(4):14411457. ［3］ Georgescu N, Lupu AR. Tumoral and normal cells treatment with highvoltage pulsed cold atmospheric plasma jets［J］. IEEE Trans Plasma Sci, 2010, 38(8):19491955. ［4］ Zirnheld JL, Zucker SN, DiSanto TM, et al. Nonthermal plasma needle: development and targeting of melanomacells［J］. IEEE Trans Plasma Sci, 2010, 38(4):948952. ［5］ Fridman G, Shereshevsky A. Floating electrode dielectric barrier discharge plasma in air promoting apoptotic behavior in melanoma skincancer cell lines［J］. Plasma Chem Plasma Process, 2007, 27(2):163176. ［6］ Sensenig R, Kalghatgi S, Cerchar E, et al. Nonthermal plasma induces apoptosis in melanoma cells via production of intracellular reactive oxygen species［J］. Ann Biomed Eng, 2011, 39(2): 674687. ［7］ Kim JY, Ballato J, Foy P, et al. Apoptosis of lung carcinoma cells induced by a flexible optical fiberbased cold Microplasma［J］. Biosens Bioelectron, 2011, 28(1):333338. ［8］ Keidar M, Walk R, Shashurin A, et al. Cold plasma selectivity and the possibilityof a paradigm shift in cancer therapy［J］. Br J Cancer, 2011, 105(9):12951301. ［9］ Kaushik NK, Kim YH, Han YG, et al. Effect of jet plasma on T98G human brain cancer cells［J］. Current Applied Physics, 2013, 13(1):176180. ［10］ Zhang XH, Li MJ, Zhou RL. Ablation of liver cancer cells in vitro by a plasma needle［J］. Appl Phys Lett, 2008, 93:2150221503. ［11］ Kim SJ, Chung TH, Bae SH, et al. Induction of apoptosis in human breast cancercells by a pulsed atmospheric pressure plasma jet［J］. Applied Physics Letters, 2010, 97(2):2370223703. ［12］ Walk RM , Snyder JA, Srinivasana P, et al. Cold atmospheric plasma for the ablative treatment of neuroblastoma［J］. J Pediat Surg, 2013, 48(1):6773. ［13］ Vandamme M, Robert E, Pesnel S, et al. Antitumor effect of plasma treatment on U87 glioma xenografts: preliminary results［J］. Plasma Processes Polym, 2010, 7(34):264273. ［14］ Georgescu N, Lupu AR. Tumoral and normal cells treatment with highvoltage pulsed cold atmospheric plasma jets［J］. IEEE Trans Plasma Sci, 2010, 38(8):19491955. ［15］ Kim GC, Kim GJ, Park SR, et al. Air plasma coupled withantibodyconjugated nanoparticles: a new weapon against cancer［J］. J Phys D: Appl Phys, 2009, 42(3):032005 ［16］ Kritzer J, Boxhammer V, Schfer A, et al. Restoration of sensitivity in chemoresistant glioma cells by cold atmospheric plasma［J］. PLoS One, 2013, 8(5):e64498. ［17］ Brullé L, Vandamme M, Riès D, et al. Effects of a non thermal plasma treatment alone or in combination with gemcitabine in a MIA PaCa2luc orthotopic pancreatic carcinoma model［J］. PLoS One, 2012, 7(12):e52653. ［18］ Dobrynin D, Fridman G, Friedman G, et al. Physical and biological mechanisms of direct plasma interaction with living tissue［J］. New J Phys, 2009, 11:115020. ［19］ Xu Y, Zou F, Zhao SS. On the mechanism of plasma inducing cell apoptosis. IEEE Trans Plasma Sci, 2010, 38(9): 24512457. ［20］ Kim GJ, Kim W, Kim KT, et al. DNA damage and mitochondria dysfunction in cell apoptosis induced by nonthermal air plasma［J］. Appl Phys Lett, 2010, 96(2):21502. ［21］ Arndt S, Wacker E, Li YF, et al. Cold atmospheric plasma, a new strategy toinduce senescence in melanoma cells［J］. Exp Dermatol, 2013, 22(4):284289. ［22］ Volotskova O, Hawley TS, Stepp MA, et al. Targeting the cancer cell cycle by cold atmospheric plasma［J］. Sci Rep, 2012, 2:636. ［23］ Vandamme M, Robert E, Lerondel S, et al. ROS implication in a new antitumor strategy based on nonthermal plasma［J］. Int J Cancer, 2012, 130(9): 21852194. ［24］ Kim CH, Bahn JH, Lee SH, et al. Induction of cell growth arrest by Atmospheric nonthermal plasma in colorectal cancer cells［J］. J Biotechnol, 2010, 150(4):530538. ［25］ Lee HJ, Shon CH, Kim YS, et al. Degradation of adhesion molecules of G361 melanoma cells by a nonthermal atmospheric pressure microplasma［J］. New J Phys, 2009, 11:115026.

[1]	王子豪, 王宇, 杨鑫, 何艺, 莫兴奎, 袁涛. 铁死亡在骨肉瘤中的分子机制及相关治疗的研究进展[J]. 国际肿瘤学杂志, 2024, 51(4): 239-244.
[2]	任露, 谢晓丽, 张坤, 王丽娟. 双氢青蒿素联合卡非佐米对多发性骨髓瘤细胞活性、增殖、凋亡的影响及机制研究[J]. 国际肿瘤学杂志, 2024, 51(3): 129-136.
[3]	张科平, 赵永生, 杨娟, 付茂勇. 绿原酸通过抑制PI3K-Akt信号通路诱导肺癌A549细胞线粒体功能障碍[J]. 国际肿瘤学杂志, 2024, 51(1): 21-28.
[4]	向玉玲, 谭佳杰, 熊远果, 赵丽蓉, 黎晨, 张洪. 脱水淫羊藿素对肝癌细胞增殖、迁移和凋亡的影响[J]. 国际肿瘤学杂志, 2023, 50(9): 513-519.
[5]	冯东旭, 吴炜, 高平发, 王军, 施丽娟, 陈大伟, 李文兵, 张美峰. miR-451通过调控Rho/ROCK1信号通路对乳腺癌细胞糖酵解及凋亡的影响[J]. 国际肿瘤学杂志, 2023, 50(8): 449-456.
[6]	吴佳丽, 张佳慧, 张萍, 肖昕悦, 李睿, 张红宇. Bcl-2 BH4选择性抑制剂BDA-366抑制NK/T细胞淋巴瘤细胞的机制研究[J]. 国际肿瘤学杂志, 2023, 50(7): 413-418.
[7]	杨娅, 王慧礼, 刘艳, 郭金凤, 王春霞, 吕敏, 山长平. GCSH基因在胃癌SNU-1细胞增殖和凋亡中的作用研究[J]. 国际肿瘤学杂志, 2023, 50(5): 257-262.
[8]	朱易, 陈健. 硫化氢在肿瘤发生发展中的作用机制及其供体抗肿瘤作用[J]. 国际肿瘤学杂志, 2023, 50(12): 729-733.
[9]	连海伟, 杨烁锐, 刘仁忠. 金松双黄酮联合CX-4945通过Notch1通路调控胶质母细胞瘤细胞增殖与凋亡的机制研究[J]. 国际肿瘤学杂志, 2022, 49(6): 321-326.
[10]	杨娅, 宁晓飞, 李炳亮, 姚慧, 山长平, 吕敏. 原花青素通过诱导活性氧产生介导抗SNU-1胃癌细胞株的作用机制研究[J]. 国际肿瘤学杂志, 2022, 49(5): 257-262.
[11]	蒋梦婷, 王家淳, 宗静. 抑制NFAT5对肺腺癌细胞增殖、侵袭、迁移及凋亡能力的影响[J]. 国际肿瘤学杂志, 2021, 48(6): 321-327.
[12]	王宪伟, 史美燕, 王凤芹, 齐福, 王朝喆, 周飞. TSA上调miR-4298靶向抑制PADI4表达在诱导U251细胞凋亡中的作用[J]. 国际肿瘤学杂志, 2021, 48(4): 193-199.
[13]	马秀珍, 卢艳, 赵冰冰, 邱宏聪, 徐勋, 韦敏. 岗松总黄酮对宫颈癌SiHa细胞迁移、侵袭及凋亡的影响[J]. 国际肿瘤学杂志, 2021, 48(4): 206-211.
[14]	李哲丰, 李洁, 赵晓婷, 岳文涛. GLDC通过PI3K/Akt/mTOR通路调控卵巢癌细胞的增殖与凋亡[J]. 国际肿瘤学杂志, 2021, 48(12): 716-722.
[15]	施玥, 李晟, 冯继锋. 急腹症风险下转移灶不可切除结直肠癌原发灶的处理方式选择[J]. 国际肿瘤学杂志, 2021, 48(11): 693-697.