国际肿瘤学杂志

国际肿瘤学杂志 ›› 2011, Vol. 38 ›› Issue (10): 794-797.

• 论著 • 上一篇    下一篇

HIV-1 Vpr体外诱导肿瘤细胞凋亡与细胞周期阻滞的研究

佟加贝, 韩金祥, 鲁艳芹   

  1. 山东省医药生物技术研究中心
  • 出版日期:2011-10-08 发布日期:2011-09-27
  • 通讯作者: 鲁艳芹，E-mail:yanqinlu@163.com E-mail:yanqinlu@163.com
  • 基金资助:

    国家高技术研究发展计划项目（2007AA021004）

Cell cycle arrest and cell apoptosis induced by Vpr in cancer cells in vitro

TONG  Jia-Bei, HAN  Jin-Xiang, LU  Yan-Qin   

  1. Laboratory of Shandong Medicinal Biotechnology Center, Jinan 250062, China
  • Online:2011-10-08 Published:2011-09-27
  • Contact: Corresponding author: LU Yan-qin, E-mail: yanqinlu@163.com E-mail:yanqinlu@163.com

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摘要： 目的研究HIV-1 Vpr诱导Hela、Lovo、HepG2细胞凋亡和G2期阻滞效果。方法构建含有HIV-1 Vpr基因的pcDNA4-Vpr重组载体，体外转染Hela、Lovo、HepG2细胞，同时设pcDNA4-CMV-EGFP质粒转染对照组、FUGENE组和空白对照组。转染后24、48、72 h，通过甲臜化合物法检测细胞增殖、流式细胞仪检测细胞周期分布，并使用ANNEXIN V/PI双染法检测细胞凋亡。结果与对照组相比，转染后24、48、72 h，Hela、Lovo、HepG2三种细胞的增殖抑制率、G2期阻滞率及细胞凋亡率均呈上升趋势(P<0.05)。Hela、Lovo、HepG2在转染后72 h的增殖抑制率分别为29.67％、27.35％、31.67％；G2期细胞周期阻滞率分别为24.9％、18.8％、32.1％；凋亡率分别为15.46％、7.7％、41.5％。结论 Vpr体外能诱导Hela、Lovo、HepG2肿瘤细胞的G2期阻滞和细胞凋亡。

Abstract: Objective To investigate the G2 cell cycle arrest and apoptosis induced by HIV-1 Vpr gene on Hela、Lovo and HepG2 cancer cells. Methods  Recombinant vector pcDNA4 –vpr was constructed by DNA recombination technology and was then transfected into Hela, Lovo and HepG2 cells. Meanwhile, pcDNA4 –EGFP plasmid group, FUGENE group and blank group was also set up as control. Ratio of cytostasis was evaluated by MTS assay 24h, 48h and 72h later, cell cycle arrest examined by flow cytometry and apoptosis detected by staining with ANNEXIN V and PI double dyes. Results  Compared to the control group, the value of inhibition ratio, G2 arrest and apoptosis of Hela, Lovo and HepG2 cells increased obviously 24h, 48h and 72h after the transfection (P<0.05). 72h after the transfection, the inhibition ratio of Hela, Lovo and HepG2 was 29.67%, 27.35% and 31.67% respectively. Percentage of G2 phase cells was 24.9%, 18.8% and 32.1% respectively. Apoptosis percentage of Hela, Lovo and HepG2 cells was 15.46%, 7.7% and 41.5% correspondingly. Conclusion  HIV-1 vpr gene can induce cell cycle G2 arrest and apoptosis of Hela, Lovo and HepG2 cell line in vitro.