国际肿瘤学杂志

国际肿瘤学杂志 ›› 2015, Vol. 42 ›› Issue (7): 504-507.doi: 10.3760/cma.j.issn.1673-422X.2015.07.007

• 论著 • 上一篇    下一篇

SIRT-1、NF-κB在非小细胞肺癌中的表达及意义

蒋中秀,刘洋,李宁,齐晓莹,张晓晔   

  1. 110022 沈阳，中国医科大学附属盛京医院第四肿瘤科
  • 出版日期:2015-07-08 发布日期:2015-05-26
  • 通讯作者: 张晓晔 E-mail:zhangxy1@sj-hospital.org
  • 基金资助:

    辽宁省自然科学基金（2014021032）

Expression and clinical significance of SIRT-1 and NF-κB in non-small cell lung cancer

Jiang Zhongxiu, Liu Yang, Li Ning, Qi Xiaoying, Zhang Xiaoye   

  1. Fourth Department of Oncology, Affiliated Shengjing Hospital of China Medical University, Shenyang 110022, China
  • Online:2015-07-08 Published:2015-05-26
  • Contact: Zhang Xiaoye E-mail:zhangxy1@sj-hospital.org

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摘要： 【摘要】目的探讨沉默交配型信息调节因子2同源蛋白1(SIRT1)、核转录因子κB（NFκB）在非小细胞肺癌(NSCLC)中的表达及其临床意义。方法采用免疫组织化学SP法，检测108例NSCLC和48例癌旁正常组织中SIRT1、NFκB的表达情况，并结合临床资料进行相关性分析。结果在NSCLC组织中，SIRT1、NFκB的阳性表达率分别为90.7%（98/108）、94.4%（102/108），均明显高于癌旁正常组织的4.2%（2/48）、16.7%（8/48），差异有统计学意义（χ2=108.237，P=0.000；χ2=96.683，P=0.000），且两者表达呈正相关（r=0.480，P=0.000）；NSCLC组织中SIRT1的表达与肿瘤大小（r=0.227，P=0.018）、TNM分期（r=0.298，P=0.002）及淋巴结转移（r=0.280，P=0.003）呈正相关，与肿瘤分化程度呈负相关（r=-0.300，P=0.002），与性别、年龄、组织学类型无关。NSCLC组织中NFκB的表达与TNM分期（r=0.256，P=0.009）及淋巴结转移（r=0.261，P=0.006）呈正相关，与肿瘤分化程度呈负相关（r=-0.235，P=0.013），与性别、年龄、组织学类型、肿瘤大小无关。结论SIRT1、NFκB在NSCLC中表达均明显高于癌旁正常组织，且与淋巴结转移、TNM分期及组织分化程度密切相关，SIRT1尚与肿瘤大小密切相关。提示SIRT1、NFκB高表达在NSCLC发生发展中可能具有重要作用。

关键词: 抗衰老酶, NF-&kappa, B, 癌, 非小细胞肺, 免疫组织化学

Abstract: ObjectiveTo investigate the expression and clinical significance of silent mating type information regulation 2 homolog1 (SIRT1) and nuclear factorκB (NFκB) in nonsmall cell lung cancer (NSCLC). MethodsThe expressions of SIRT1 and NFκB were evaluated by immunohistochemistry in 108 selected cases of primary NSCLC and 48 samples of paracarcinoma normal tissue. The relationships of the expressions of SIRT1 and NFκB and clinical pathological features were analyzed, respectively. ResultsImmunohistochemical results showed that the positive expression rates of SIRT1 and NFκB in NSCLC tissue were 90.7% (98/108) and 94.4% (102/108), respectively, significantly higher than those in normal lung tissue 4.2% (2/48), 16.7% (8/48), χ2=108.237, P=0.000; χ2=96.683, P=0.000, and the expression of SIRT1 and NFκB showed a positive correlation (r=0.480, P=0.001). There were significant positive correlations between the expression of SIRT1 and tumor size （r=0.227, P=0.018）, TNM stage （r=0.298, P=0.002） and lymph node metastasis （r=0.280, P=0.003）, and there was negative correlation between the expression of SIRT1 and differentiation （r=-0.300, P=0.002）, and there were no correlation between the expression of SIRT1 and sex, age and histological type in NSCLC tissues. There were significant positive correlation between the expression of NFκB and TNM stage （r=0.256, P=0.009） and lymph node metastasis （r=0.261, P=0.006）, and there was negative correlation between the expression of NFκB and differentiation （r=-0.235, P=0.013）, and there were no correlation between the expression of NFκB and sex, age, histological type and tumor size in NSCLC tissues. ConclusionThe positive expression rates of SIRT1 and NFκB in NSCLC tissue are significantly higher than those in normal lung tissue, and they are related to TNM stage, lymph node metastasis and differentiation, and the former is also related to tumor size. High expression of SIRT1 and NFκB may play important roles in the occurrence and development of NSCLC．

Key words: irtuins, NF-kappa B, Carcinoma, non-small-cell lung, Immunohistochemistry