国际肿瘤学杂志

国际肿瘤学杂志 ›› 2015, Vol. 42 ›› Issue (7): 485-487.doi: 10.3760/cma.j.issn.1673-422X.2015.07.002

• 论著 • 上一篇    下一篇

胃肿瘤抗原致敏的脐血DC联合CIK对胃癌细胞株SGC-7901杀伤活性的研究

薛克伟,李贵新,李新新,郭迎雪,李敖,王文浩   

  1. 261053 潍坊医学院研究生院（薛克伟、李新新、郭迎雪）；潍坊医学院附属医院肿瘤科（李贵新、李敖、王文浩）
  • 出版日期:2015-07-08 发布日期:2015-05-26
  • 通讯作者: 李贵新 E-mail:liguixin@wfmc.edu.com

Effect of umbilical cord blood dendritic cells induced by gastric cancer antigen combined with CIK cells in gastric cancer cell lines SGC-7901

Xue Kewei, Li Guixin, Li Xinxin, Guo Yingxue, Li Ao, Wang Wenhao   

  1. Graduate School， Weifang Medical University, Weifang 261053, China
  • Online:2015-07-08 Published:2015-05-26
  • Contact: Li Guixin E-mail:liguixin@wfmc.edu.com

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摘要： 目的探讨体外胃肿瘤抗原致敏脐血DC联合细胞因子诱导的杀伤细胞（CIK）对胃癌细胞株SGC7901的杀伤作用。方法分离脐血单个核细胞培养DC细胞和CIK细胞。流式细胞仪检测成熟DC细胞表面抗原CD83、CD86、CD11c 及CIK细胞表面抗原CD3、CD56、CD4、CD8、CD16表达。致敏DCCIK、非致敏DCCIK、CIK作为效应细胞，SGC7901作为靶细胞，利用乳酸脱氢酶（LDH）释放法检测致敏DCCIK、脐血DCCIK、CIK分别在效靶比10∶1、20∶1、40∶1时对胃癌细胞的杀伤活性。结果成熟脐血DC表面抗原CD83+CD86+、CD11c+CD83+、CD86+CD11c+表达率分别为（75.4±2.1）%、（79.3±1.4）%、（80.2±2.6）%。致敏脐血DC表面表达率分别为（77.7±1.5)%、(82.6±1.9)%、(76.9±2.6)%，二者差异无统计学意义（t=1.526，P＞0.05；t=0.958，P＞0.05；t=1.049，P＞0.05）。成熟CIK中CD4+细胞占(22.8±1.3)%，CD8+细胞占(77.3±1.8)%，CD3+CD56+CD16+细胞占(24.5±2.1)%。致敏DCCIK、DCCIK、CIK都对胃癌细胞有杀伤作用，致敏DCCIK在效靶比为10∶1、20∶1、40∶1时杀瘤活性分别为（37.68±1.49）%、（41.67±0.90）%、（42.71±0.98）%，在效靶比为40∶1时杀瘤活性最强，DCCIK组分别为（36.77±0.46）%、（38.94±0.95）%、（41.15±0.89）%，CIK组分别为（34.74±1.01）%、（37.76±0.43）%、（39.65±0.79）%，三组间差异有统计学意义（F=5.92，P＜0.05；F=19.13，P＜0.05；F=8.88，P＜0.05）。结论胃肿瘤抗原致敏脐血DC可明显增强DCCIK的杀瘤活性，致敏脐血DCCIK在效靶比为40∶1时杀瘤活性最强。

关键词: 胃肿瘤, 树突细胞, 杀伤细胞, 淋巴因子激活

Abstract: ObjectiveTo investigate the effect of umbilical cord blood dendritic cells (DCs) induced by gastric cancer antigen combined with cytokine induced killer (CIK) cells in gastric cancer cell lines SGC7901 in vitro. MethodsMononuclear cells from umbilical cord blood were used to create DCs and CIKs. The cell surface antigen expression of the mature DCs such as CD83, CD86, CD11c and the cell surface antigen of CIKs such as CD3, CD56, CD4, CD8, CD16 were detected using flow cytometry. Sensitized DCsCIKs, DCsCIKs, CIKs as effective cells, and SGC7901 as target cells, the killing activities of these effective cells were tested with LDH release, which the number ratio of cells between effective cells and SGC7901 cells were 10∶1, 20∶1, 40∶1, respectively. ResultsThe cell surface antigen expressions of the mature DCs, such as CD83+CD86+, CD11c+CD83+, CD86+CD11c+ were （75.4±2.1）%, （79.3±1.4）%, （80.2±2.6）%, respectively. The mature sensitiveDCs surface antigen expressions, such as CD83+CD86+, CD11c+CD83+, CD86+CD11c+, were （77.7±1.5)%, (82.6±1.9)%, (76.9±2.6)%, respectively. There was no statistical significance about the surface antigen expression between DCs and  sensitiveDCs （t=1.526, P＞0.05; t=0.958, P＞0.05; t=1.049, P＞0.05）. The CIKs surface antigen expressions, such as CD4+, CD8+, CD3+CD56+CD16+, were (22.8±1.3)%, (77.3±1.8)%, (24.5±2.1)%, respectively. The results suggested that the killing effect of the three kinds of combination cells on gastric cancer cells was different. The number ratio of cells between sensitiveDCs and SGC7901 cells were 10∶1, 20∶1, 40∶1, which the killing activities of sensitiveDCsCIKs against SGC7901 were (37.68±1.49）%, (41.67±0.90）%, (42.71±0.98）%, respectively. The killing activity of sensitiveDCsCIKs was the highest when the ratio of cells between sensitiveDCs and SGC7901 cells were 40∶1. The killing activities of  DCCIKs were (36.77±0.46）%, (38.94±0.95）%, (41.15±0.89）%, respectively.  The killing activities of  CIKs  were （34.74±1.01）%, （37.76±0.43）%, （39.65±0.79）%, respectively. There were statistically significant differences among the three groups （F=5.92, P＜0.05; F=19.13, P＜0.05; F=8.88, P＜0.05）. ConclusionThe tumor killing activity of CIK is enhanced obviously by umbilical cord blood DCs which is sensitized by gastric cancer tumor antigen. There is the highest killing activity when the number ratio of cells between sensitiveDCCIK and SGC7901 cells is 40∶1.

Key words: Stomach neoplasms, Dendritic cells, Killer cells, lymphokineactivated